PMID- 3819582
OWN - NLM
STAT- MEDLINE
DCOM- 19870420
LR  - 20180206
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 109
IP  - 4
DP  - 1987 Apr
TI  - Assessment of the hydration state of sickle cells by phthalate ester density 
      distribution.
PG  - 486-94
AB  - Intracellular hemoglobin S (Hb SS) concentration, a function of cell hydration, 
      has a major influence on the rate of Hb SS polymerization and, therefore, 
      cellular sickling. To determine the density distribution of homozygous sickle 
      hemoglobin cells as a function of cell hydration, cells were incubated in 
      autologous plasma buffer mixtures with final osmolalities ranging from 195 to 490 
      mosm/kg at ambient Po2. The density distribution of the cells was determined by 
      differential flotation on 20 mixtures of di-n-butyl and dimethyl phthalates with 
      specific gravities of 1.062 to 1.142. Mean cell hemoglobin concentration (MCHC) 
      and mean cell volume (MCV) were determined by standard manual procedures. Cell 
      shape was assessed by scanning electron microscopy (SEM), and the axial ratio 
      (L/W) of the elliptical dense cell fraction measured by an image analyzer 
      interfaced with a computer. The density distribution of normal red blood cells 
      lies within a narrow 1.090 to 1.118 gm/ml density band with the middle or 
      transitional 60% (T60) of the cells occupying a density range of 0.0067 +/- 
      0.0007 gm/ml (+/- SD). The density distribution of sickle cells shows a broader 
      density band of 1.064 to 1.134 gm/ml, and the T60 was 0.0139 +/- 0.0022 gm/ml. 
      The mean T60 did not change with osmotic variation but the mean T60 of Hb SS 
      cells was significantly greater (P less than 0.005). MCHC and 1/MCV varied 
      directly with the median density of the density distribution. By linear 
      regression analysis and Ponder's osmotic equation, it is evident that sickle 
      cells exhibit restricted volume increases in hypotonic media.(ABSTRACT TRUNCATED 
      AT 250 WORDS)
FAU - Kurantsin-Mills, J
AU  - Kurantsin-Mills J
FAU - Jacobs, H M
AU  - Jacobs HM
FAU - Lessin, L S
AU  - Lessin LS
LA  - eng
GR  - 15160/PHS HHS/United States
GR  - N01-HB-7-2994/HB/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Phthalic Acids)
SB  - IM
MH  - Anemia, Sickle Cell/*blood
MH  - Body Water/*analysis
MH  - Centrifugation, Density Gradient/methods
MH  - Erythrocyte Indices
MH  - Erythrocytes, Abnormal/analysis/*cytology
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - Osmolar Concentration
MH  - Phthalic Acids
EDAT- 1987/04/01 00:00
MHDA- 1987/04/01 00:01
CRDT- 1987/04/01 00:00
PHST- 1987/04/01 00:00 [pubmed]
PHST- 1987/04/01 00:01 [medline]
PHST- 1987/04/01 00:00 [entrez]
AID - 0022-2143(87)90122-3 [pii]
PST - ppublish
SO  - J Lab Clin Med. 1987 Apr;109(4):486-94.