PMID- 38197208
OWN - NLM
STAT- MEDLINE
DCOM- 20240111
LR  - 20240113
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 42
IP  - 1
DP  - 2024 Jan 8
TI  - [Determination of 15 preservative allergens in cosmetics by gas 
      chromatography-tandem mass spectrometry].
PG  - 75-83
LID - 10.3724/SP.J.1123.2023.04010 [doi]
AB  - Most preservatives are irritating and can easily induce skin sensitivities. 
      Therefore, both domestic and international regulations impose clear restrictions 
      on the use of preservatives in cosmetics. Herein, gas chromatography-tandem mass 
      spectrometry (GC-MS/MS) was employed to simultaneously analyze the levels of 15 
      preservative allergens in cosmetics. Further, a precise identification approach 
      based on a two-column retention index and mass spectrometry matching degree was 
      developed. Cosmetic samples were extracted via acetonitrile vortex ultrasound 
      extraction and then dehydrated with anhydrous MgSO(4). The preservative allergens 
      were separated on two columns, namely, DB-5MS and DB-WAX. Targets were identified 
      using electron impact ionization (EI) source and the multiple reaction monitoring 
      (MRM) mode and characterized using a retention index calibrated by a series of 
      n-alkane standards. Following two tests, the LODs for the 15 preservative 
      allergens on the DB-5MS column were in the range of 0.02-0.2 mg/kg, while those 
      for 12 preservative allergens on the DB-WAX column were in the range of 0.01-20 
      mg/kg. The preservative allergens on the DB-5MS and DB-WAX columns demonstrated 
      strong correlations, with all correlation coefficients exceeding 0.99. The 
      recoveries for the 15 preservative allergens were in the range of 70.1%-129.8% at 
      low, medium, and high levels, and the relative standard deviations (RSDs) were 
      all below 15% (n=6) when using water, lotion, facial mask, and cream as the 
      representative matrix. Next, 80 batches of genuine samples were tested using the 
      established method. Isopropyl 4-hydroxybenzoate, a prohibited preservative, was 
      detected in two sample batches using the DB-5MS and DB-WAX columns. Additionally, 
      11 and 10 restricted preservative allergens were identified on the DB-5MS and 
      DB-WAX columns, respectively. The test results indicate that the double-column 
      system approach offers excellent accuracy, effectively preventing false-positive 
      and false-negative results, and can detect the 15 preservative allergens in 
      cosmetics. The use of the retention index for the qualitative detection of these 
      preservative allergens offers valuable options for non-targeted screening and 
      meeting regulatory criteria.
FAU - Han, Weina
AU  - Han W
AD  - Key Laboratory of Food Quality and Safety, State Administration for Market 
      Regulation, National Institutes for Food and Drug Control, Beijing 100050, China.
AD  - China Pharmaceutical University, Nanjing 210009, China.
FAU - Liu, Tongtong
AU  - Liu T
AD  - Key Laboratory of Food Quality and Safety, State Administration for Market 
      Regulation, National Institutes for Food and Drug Control, Beijing 100050, China.
FAU - Wang, Lulu
AU  - Wang L
AD  - Key Laboratory of Food Quality and Safety, State Administration for Market 
      Regulation, National Institutes for Food and Drug Control, Beijing 100050, China.
FAU - Hou, Xiaoyu
AU  - Hou X
AD  - China Pharmaceutical University, Nanjing 210009, China.
FAU - Cao, Jin
AU  - Cao J
AD  - Key Laboratory of Food Quality and Safety, State Administration for Market 
      Regulation, National Institutes for Food and Drug Control, Beijing 100050, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Allergens)
RN  - 0 (Alkanes)
RN  - 0 (Cosmetics)
RN  - 0 (Preservatives, Pharmaceutical)
SB  - IM
MH  - *Allergens
MH  - Tandem Mass Spectrometry
MH  - Gas Chromatography-Mass Spectrometry
MH  - Alkanes
MH  - *Cosmetics
MH  - Preservatives, Pharmaceutical
PMC - PMC10782272
OTO - NOTNLM
OT  - cosmetics
OT  - gas chromatography-tandem mass spectrometry (GC-MS/MS)
OT  - multiple reaction monitoring (MRM)
OT  - preservative allergens
OT  - retention index (RI)
EDAT- 2024/01/10 06:41
MHDA- 2024/01/11 07:43
PMCR- 2024/01/08
CRDT- 2024/01/10 04:33
PHST- 2024/01/11 07:43 [medline]
PHST- 2024/01/10 06:41 [pubmed]
PHST- 2024/01/10 04:33 [entrez]
PHST- 2024/01/08 00:00 [pmc-release]
AID - 10.3724/SP.J.1123.2023.04010 [doi]
PST - ppublish
SO  - Se Pu. 2024 Jan 8;42(1):75-83. doi: 10.3724/SP.J.1123.2023.04010.