PMID- 38198011 OWN - NLM STAT- MEDLINE DCOM- 20240111 LR - 20240213 IS - 1432-2242 (Electronic) IS - 0040-5752 (Linking) VI - 137 IP - 1 DP - 2024 Jan 10 TI - Development, identification, and utilization of wheat-tetraploid Thinopyrum elongatum 4EL translocation lines resistant to stripe rust. PG - 17 LID - 10.1007/s00122-023-04525-5 [doi] AB - The new stripe rust resistance gene Yr4EL in tetraploid Th. elongatum was identified and transferred into common wheat via 4EL translocation lines. Tetraploid Thinopyrum elongatum is a valuable genetic resource for improving the resistance of wheat to diseases such as stripe rust, powdery mildew, and Fusarium head blight. We previously reported that chromosome 4E of the 4E (4D) substitution line carries all-stage stripe rust resistance genes. To optimize the utility of these genes in wheat breeding programs, we developed translocation lines by inducing chromosomal structural changes through (60)Co-gamma irradiation and developing monosomic substitution lines. In total, 53 plants with different 4E chromosomal structural changes were identified. Three homozygous translocation lines (T4DS.4EL, T5AL.4EL, and T3BL.4EL) and an addition translocation line (T5DS.4EL) were confirmed by the genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), FISH-painting, and wheat 55 K SNP array analyses. These four translocation lines, which contained chromosome arm 4EL, exhibited high stripe rust resistance. Thus, a resistance gene (tentatively named Yr4EL) was localized to the chromosome arm 4EL of tetraploid Th. elongatum. For the application of marker-assisted selection (MAS), 32 simple sequence repeat (SSR) markers were developed, showing specific amplification on the chromosome arm 4EL and co-segregation with Yr4EL. Furthermore, the 4DS.4EL line could be selected as a good pre-breeding line that better agronomic traits than other translocation lines. We transferred Yr4EL into three wheat cultivars SM482, CM42, and SM51, and their progenies were all resistant to stripe rust, which can be used in future wheat resistance breeding programs. CI - (c) 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Gong, Biran AU - Gong B AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Chen, Linfeng AU - Chen L AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Zhang, Hao AU - Zhang H AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Zhu, Wei AU - Zhu W AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Xu, Lili AU - Xu L AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Cheng, Yiran AU - Cheng Y AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Wang, Yi AU - Wang Y AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Zeng, Jian AU - Zeng J AD - College of Resources, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Fan, Xing AU - Fan X AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Sha, Lina AU - Sha L AD - College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Zhang, Haiqin AU - Zhang H AD - College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Chen, Guoyue AU - Chen G AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Zhou, Yonghong AU - Zhou Y AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. FAU - Kang, Houyang AU - Kang H AUID- ORCID: 0000-0002-0561-5413 AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. houyang.kang@sicau.edu.cn. AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. houyang.kang@sicau.edu.cn. FAU - Wu, Dandan AU - Wu D AUID- ORCID: 0000-0003-2622-4742 AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. wudandan@sicau.edu.cn. AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. wudandan@sicau.edu.cn. LA - eng GR - NK20220607/Major Program of National Agricultural Science and Technology of China/ GR - No. 31971883/National Natural Science Foundation of China/ GR - No. 32200180/National Natural Science Foundation of China/ GR - 2023NSFSC1995/Sichuan Province Science and Technology Support Program/ GR - 2022YFH0069/Science and Technology Bureau of Sichuan Province/ GR - 2022NSFSC1671/Science and Technology Department of Sichuan Province/ GR - 2022-YF05-00449-SN/Science and Technology Bureau of Chengdu City/ PT - Journal Article DEP - 20240110 PL - Germany TA - Theor Appl Genet JT - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JID - 0145600 SB - IM MH - *Triticum/genetics MH - In Situ Hybridization, Fluorescence MH - Plant Breeding MH - Tetraploidy MH - Poaceae/genetics MH - *Basidiomycota EDAT- 2024/01/10 12:43 MHDA- 2024/01/11 07:41 CRDT- 2024/01/10 11:21 PHST- 2023/10/16 00:00 [received] PHST- 2023/12/11 00:00 [accepted] PHST- 2024/01/11 07:41 [medline] PHST- 2024/01/10 12:43 [pubmed] PHST- 2024/01/10 11:21 [entrez] AID - 10.1007/s00122-023-04525-5 [pii] AID - 10.1007/s00122-023-04525-5 [doi] PST - epublish SO - Theor Appl Genet. 2024 Jan 10;137(1):17. doi: 10.1007/s00122-023-04525-5.