PMID- 38198228
OWN - NLM
STAT- MEDLINE
DCOM- 20240418
LR  - 20240418
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 118
IP  - 2
DP  - 2024 Apr
TI  - The maize PLASTID TERMINAL OXIDASE (PTOX) locus controls the carotenoid content 
      of kernels.
PG  - 457-468
LID - 10.1111/tpj.16618 [doi]
AB  - Carotenoids perform a broad range of important functions in humans; therefore, 
      carotenoid biofortification of maize (Zea mays L.), one of the most highly 
      produced cereal crops worldwide, would have a global impact on human health. 
      PLASTID TERMINAL OXIDASE (PTOX) genes play an important role in carotenoid 
      metabolism; however, the possible function of PTOX in carotenoid biosynthesis in 
      maize has not yet been explored. In this study, we characterized the maize PTOX 
      locus by forward- and reverse-genetic analyses. While most higher plant species 
      possess a single copy of the PTOX gene, maize carries two tandemly duplicated 
      copies. Characterization of mutants revealed that disruption of either copy 
      resulted in a carotenoid-deficient phenotype. We identified mutations in the PTOX 
      genes as being causal of the classic maize mutant, albescent1. Remarkably, 
      overexpression of ZmPTOX1 significantly improved the content of carotenoids, 
      especially beta-carotene (provitamin A), which was increased by ~threefold, in maize 
      kernels. Overall, our study shows that maize PTOX locus plays an important role 
      in carotenoid biosynthesis in maize kernels and suggests that fine-tuning the 
      expression of this gene could improve the nutritional value of cereal grains.
CI  - (c) 2024 Society for Experimental Biology and John Wiley & Sons Ltd.
FAU - Nie, Yongxin
AU  - Nie Y
AD  - State Key Laboratory of Crop Biology, College of Life Sciences, Shandong 
      Agricultural University, Taian, 271018, China.
FAU - Wang, Hui
AU  - Wang H
AD  - Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, 
      China.
FAU - Zhang, Guan
AU  - Zhang G
AD  - State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land 
      in Northern China, the Institute of Agricultural Resources and Regional Planning, 
      Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
FAU - Ding, Haiping
AU  - Ding H
AD  - State Key Laboratory of Crop Biology, College of Life Sciences, Shandong 
      Agricultural University, Taian, 271018, China.
FAU - Han, Beibei
AU  - Han B
AD  - State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land 
      in Northern China, the Institute of Agricultural Resources and Regional Planning, 
      Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
FAU - Liu, Lei
AU  - Liu L
AUID- ORCID: 0000-0002-5095-7244
AD  - National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, 
      Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Shi, Jian
AU  - Shi J
AD  - Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, 
      China.
FAU - Du, Jiyuan
AU  - Du J
AD  - State Key Laboratory of Crop Biology, College of Life Sciences, Shandong 
      Agricultural University, Taian, 271018, China.
FAU - Li, Xiaohu
AU  - Li X
AUID- ORCID: 0000-0001-8439-9159
AD  - State Key Laboratory of Crop Biology, College of Life Sciences, Shandong 
      Agricultural University, Taian, 271018, China.
FAU - Li, Xinzheng
AU  - Li X
AD  - State Key Laboratory of Crop Biology, College of Life Sciences, Shandong 
      Agricultural University, Taian, 271018, China.
FAU - Zhao, Yajie
AU  - Zhao Y
AD  - State Key Laboratory of Crop Biology, College of Life Sciences, Shandong 
      Agricultural University, Taian, 271018, China.
FAU - Zhang, Xiaocong
AU  - Zhang X
AD  - Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 
      100081, China.
FAU - Liu, Changlin
AU  - Liu C
AD  - Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 
      100081, China.
FAU - Weng, Jianfeng
AU  - Weng J
AD  - Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 
      100081, China.
FAU - Li, Xinhai
AU  - Li X
AD  - Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 
      100081, China.
FAU - Zhang, Xiansheng
AU  - Zhang X
AD  - State Key Laboratory of Crop Biology, College of Life Sciences, Shandong 
      Agricultural University, Taian, 271018, China.
FAU - Zhao, Xiangyu
AU  - Zhao X
AD  - State Key Laboratory of Crop Biology, College of Life Sciences, Shandong 
      Agricultural University, Taian, 271018, China.
FAU - Pan, Guangtang
AU  - Pan G
AD  - Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, 
      China.
FAU - Jackson, David
AU  - Jackson D
AD  - Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 11724, USA.
FAU - Li, Qin-Bao
AU  - Li QB
AD  - USDA-ARS, Chemistry Research Unit, Gainesville, Florida, 32608, USA.
FAU - Stinard, Philip S
AU  - Stinard PS
AD  - USDA-ARS, Maize Genetics Cooperation Stock Center, Urbana, Illinois, 61801, USA.
FAU - Arp, Jennifer
AU  - Arp J
AD  - University of Illinois at Urbana-Champaign, Department of Crop Sciences, Urbana, 
      Illinois, 61801, USA.
AD  - Bayer Crop Science 700 Chesterfield Parkway West, Chesterfield, Missouri, 63017, 
      USA.
FAU - Sachs, Martin M
AU  - Sachs MM
AD  - USDA-ARS, Maize Genetics Cooperation Stock Center, Urbana, Illinois, 61801, USA.
AD  - University of Illinois at Urbana-Champaign, Department of Crop Sciences, Urbana, 
      Illinois, 61801, USA.
FAU - Moose, Steven
AU  - Moose S
AD  - University of Illinois at Urbana-Champaign, Department of Crop Sciences, Urbana, 
      Illinois, 61801, USA.
FAU - Hunter, Charles T
AU  - Hunter CT
AD  - USDA-ARS, Chemistry Research Unit, Gainesville, Florida, 32608, USA.
FAU - Wu, Qingyu
AU  - Wu Q
AUID- ORCID: 0000-0003-3064-2445
AD  - State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land 
      in Northern China, the Institute of Agricultural Resources and Regional Planning, 
      Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
FAU - Zhang, Zhiming
AU  - Zhang Z
AUID- ORCID: 0000-0001-5112-8387
AD  - State Key Laboratory of Crop Biology, College of Life Sciences, Shandong 
      Agricultural University, Taian, 271018, China.
LA  - eng
GR  - 32171925/National Natural Science Foundation of China/
GR  - U21A20210/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20240110
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - EC 1.- (Oxidoreductases)
RN  - 36-88-4 (Carotenoids)
RN  - 01YAE03M7J (beta Carotene)
SB  - IM
MH  - Humans
MH  - *Oxidoreductases/genetics/metabolism
MH  - *Zea mays/genetics/metabolism
MH  - Carotenoids/metabolism
MH  - beta Carotene/metabolism
MH  - Edible Grain/genetics/metabolism
MH  - Plastids/genetics/metabolism
OTO - NOTNLM
OT  - albescent1
OT  - biofortification
OT  - carotene
OT  - provitamin A
EDAT- 2024/01/10 18:42
MHDA- 2024/04/18 06:44
CRDT- 2024/01/10 12:23
PHST- 2023/12/16 00:00 [revised]
PHST- 2023/03/20 00:00 [received]
PHST- 2023/12/20 00:00 [accepted]
PHST- 2024/04/18 06:44 [medline]
PHST- 2024/01/10 18:42 [pubmed]
PHST- 2024/01/10 12:23 [entrez]
AID - 10.1111/tpj.16618 [doi]
PST - ppublish
SO  - Plant J. 2024 Apr;118(2):457-468. doi: 10.1111/tpj.16618. Epub 2024 Jan 10.