PMID- 38199544
OWN - NLM
STAT- MEDLINE
DCOM- 20240222
LR  - 20240222
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 259
IP  - Pt 2
DP  - 2024 Feb
TI  - A ternary composite hydrogel based on sodium alginate, carboxymethyl cellulose 
      and copper-doped 58S bioactive glass promotes cutaneous wound healing in vitro 
      and in vivo.
PG  - 129260
LID - S0141-8130(24)00063-1 [pii]
LID - 10.1016/j.ijbiomac.2024.129260 [doi]
AB  - Hydrogels offer a novel approach to wound repair. In this study, we synthesized a 
      ternary composite using sodium alginate (SA), carboxymethyl cellulose (CMC) and 
      copper-doped 58S bioactive glass (BG). According to our mechanical testing 
      results, the composite made of 7 wt% CMC and 7 wt% BG (SA-7CMC-7BG) showed 
      optimal properties. In addition, our in vitro studies revealed the 
      biocompatibility and bioactivity of SA-7CMC-7BG, with a negative zeta potential 
      of -31.7 mV. Scanning electron microscope (SEM) images showed 273-mum-diameter 
      pores, cell adhesion, and anchoring. The SA-7CMC-7BG closed 90.4 % of the 
      mechanical scratch after 2 days. An in vivo wound model using Wistar rats showed 
      that SA-7CMC-7BG promoted wound healing, with 85.57 % of the wounds healed after 
      14 days. Treatment with the SA-7CMC-7BG hydrogel caused a 1.6-, 65-, and 
      1.87-fold increase in transforming growth factor beta (TGF-beta), Col I, and 
      vascular endothelial growth factor (VEGF) expression, respectively that prevents 
      fibrosis and promotes angiogenesis. Furthermore, interleukin 1beta (IL-1beta) 
      expression was downregulated by 1.61-fold, indicating an anti-inflammatory effect 
      of SA-7CMC-7BG. We also observed an increase in epidermal thickness, the number 
      of fibroblast cells, and collagen deposition, which represent complementary 
      pathology results confirming the effectiveness of the SA-7CMC-7BG hydrogel in 
      cutaneous wound healing.
CI  - Copyright (c) 2024 Elsevier B.V. All rights reserved.
FAU - Kohoolat, Ghazaleh
AU  - Kohoolat G
AD  - Department of Materials Science & Engineering, Faculty of Engineering & 
      Technology, Tarbiat Modares University, P. O. Box: 14115-143, Tehran, Iran.
FAU - Alizadeh, Parvin
AU  - Alizadeh P
AD  - Department of Materials Science & Engineering, Faculty of Engineering & 
      Technology, Tarbiat Modares University, P. O. Box: 14115-143, Tehran, Iran. 
      Electronic address: p-alizadeh@modares.ac.ir.
FAU - Motesadi Zarandi, Fatemeh
AU  - Motesadi Zarandi F
AD  - Department of Materials Science & Engineering, Faculty of Engineering & 
      Technology, Tarbiat Modares University, P. O. Box: 14115-143, Tehran, Iran.
FAU - Rezaeipour, Yashar
AU  - Rezaeipour Y
AD  - Department of Materials Science & Engineering, Faculty of Engineering & 
      Technology, Tarbiat Modares University, P. O. Box: 14115-143, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20240108
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (bioactive glass 58S)
RN  - K679OBS311 (Carboxymethylcellulose Sodium)
RN  - 789U1901C5 (Copper)
RN  - 0 (Hydrogels)
RN  - 0 (Alginates)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Wound Healing
MH  - *Carboxymethylcellulose Sodium/pharmacology
MH  - Copper/pharmacology
MH  - Hydrogels/pharmacology
MH  - Alginates/pharmacology
MH  - Vascular Endothelial Growth Factor A/pharmacology
MH  - Rats, Wistar
MH  - *Glass
OTO - NOTNLM
OT  - Bioactive glass
OT  - Hydrogels
OT  - Sodium alginate
OT  - Wound healing
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/01/11 00:42
MHDA- 2024/02/22 06:43
CRDT- 2024/01/10 20:19
PHST- 2023/08/14 00:00 [received]
PHST- 2023/12/26 00:00 [revised]
PHST- 2024/01/03 00:00 [accepted]
PHST- 2024/02/22 06:43 [medline]
PHST- 2024/01/11 00:42 [pubmed]
PHST- 2024/01/10 20:19 [entrez]
AID - S0141-8130(24)00063-1 [pii]
AID - 10.1016/j.ijbiomac.2024.129260 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2024 Feb;259(Pt 2):129260. doi: 
      10.1016/j.ijbiomac.2024.129260. Epub 2024 Jan 8.