PMID- 38201587
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240114
IS  - 2072-6694 (Print)
IS  - 2072-6694 (Electronic)
IS  - 2072-6694 (Linking)
VI  - 16
IP  - 1
DP  - 2023 Dec 28
TI  - Phospholipase A2 Group IIA Is Associated with Inflammatory Hepatocellular 
      Adenoma.
LID - 10.3390/cancers16010159 [doi]
LID - 159
AB  - Although benign hepatocellular adenomas (HCA) are very rare, recent observations 
      have shown their occurrence in patients with diabetes mellitus. Consequently, 
      most of these cases are treated by resection due to concerns regarding their 
      potential progression to hepatocarcinoma (HCC). This decision is largely driven 
      by the limited number of studies on HCC subtyping and the lack of molecular and 
      biological insights into the carcinogenic potential of benign tumors. This study 
      aimed to comprehensively investigate the subtype classification of HCA and to 
      compare and analyze gene expression profiling between HCA and HCC tissues. One 
      fresh inflammatory HCA (I-HCA), three non-B non-C HCCs, two hepatitis B 
      virus-HCCs, and one normal liver tissue sample were subjected to single-cell RNA 
      sequencing (scRNA-seq). Comparative analysis of scRNA-seq among different tissues 
      showed that phospholipase A2 group IIA (PLA2G2A) mRNA was specifically expressed 
      in I-HCA, following RNA-seq analysis in formalin-fixed paraffin-embedded tissues 
      from other HCAs. Immunohistochemistry using the PLA2G2A antibody in these tissues 
      indicated that the positive reaction was mainly observed in hepatocytes of I-HCAs 
      and stromal cells surrounding the tumor tissue in HCC were also stained. 
      According to a clinical database, PLA2G2A expression in HCC does not correlate 
      with poor prognosis. This finding may potentially help develop a new definition 
      for I-HCA, resulting in a significant clinical contribution, but it requires 
      validation with other fresh HCA samples.
FAU - Iwabuchi, Sadahiro
AU  - Iwabuchi S
AUID- ORCID: 0000-0003-0434-3054
AD  - Department of Molecular Pathophysiology, Institute of Advanced Medicine, Wakayama 
      Medical University, Wakayama, Wakayama 641-0011, Japan.
FAU - Takahashi, Kenta
AU  - Takahashi K
AUID- ORCID: 0000-0002-0910-4514
AD  - Department of Human Pathology, Graduate School of Medicine, Kanazawa University, 
      Ishikawa, Kanazawa 920-0934, Japan.
FAU - Kawaguchi, Kazunori
AU  - Kawaguchi K
AUID- ORCID: 0000-0002-4927-1704
AD  - Department of Gastroenterology, Kanazawa University Hospital, Ishikawa, Kanazawa 
      920-0934, Japan.
FAU - Nagatsu, Akihisa
AU  - Nagatsu A
AUID- ORCID: 0000-0002-4414-5530
AD  - Department of Gastroenterological Surgery I, Hokkaido University Graduate School 
      of Medicine, Hokkaido, Sapporo 060-8648, Japan.
FAU - Imafuku, Tadashi
AU  - Imafuku T
AD  - Department of Molecular Pathophysiology, Institute of Advanced Medicine, Wakayama 
      Medical University, Wakayama, Wakayama 641-0011, Japan.
FAU - Shichino, Shigeyuki
AU  - Shichino S
AD  - Division of Molecular Regulation of Inflammatory and Immune Disease, Research 
      Institute for Biomedical Sciences, Tokyo University of Science, Chiba, Noda 
      278-8510, Japan.
FAU - Matsushima, Kouji
AU  - Matsushima K
AD  - Division of Molecular Regulation of Inflammatory and Immune Disease, Research 
      Institute for Biomedical Sciences, Tokyo University of Science, Chiba, Noda 
      278-8510, Japan.
FAU - Taketomi, Akinobu
AU  - Taketomi A
AUID- ORCID: 0000-0002-5238-3678
AD  - Department of Gastroenterological Surgery I, Hokkaido University Graduate School 
      of Medicine, Hokkaido, Sapporo 060-8648, Japan.
FAU - Honda, Masao
AU  - Honda M
AUID- ORCID: 0000-0003-3050-5854
AD  - Department of Gastroenterology, Kanazawa University Hospital, Ishikawa, Kanazawa 
      920-0934, Japan.
FAU - Hashimoto, Shinichi
AU  - Hashimoto S
AD  - Department of Molecular Pathophysiology, Institute of Advanced Medicine, Wakayama 
      Medical University, Wakayama, Wakayama 641-0011, Japan.
LA  - eng
GR  - JP20K07653/Japan Society for the Promotion of Science/
GR  - JP21gm6210025/Japan Agency for Medical Research and Development/
PT  - Journal Article
DEP - 20231228
PL  - Switzerland
TA  - Cancers (Basel)
JT  - Cancers
JID - 101526829
PMC - PMC10778238
OTO - NOTNLM
OT  - Pla2g2a
OT  - hepatocellular adenoma
OT  - hepatocellular carcinoma
OT  - single-cell RNA sequencing
COIS- The authors declare no conflicts of interest.
EDAT- 2024/01/11 07:42
MHDA- 2024/01/11 07:43
PMCR- 2023/12/28
CRDT- 2024/01/11 01:06
PHST- 2023/11/30 00:00 [received]
PHST- 2023/12/26 00:00 [revised]
PHST- 2023/12/27 00:00 [accepted]
PHST- 2024/01/11 07:43 [medline]
PHST- 2024/01/11 07:42 [pubmed]
PHST- 2024/01/11 01:06 [entrez]
PHST- 2023/12/28 00:00 [pmc-release]
AID - cancers16010159 [pii]
AID - cancers-16-00159 [pii]
AID - 10.3390/cancers16010159 [doi]
PST - epublish
SO  - Cancers (Basel). 2023 Dec 28;16(1):159. doi: 10.3390/cancers16010159.