PMID- 38201888 OWN - NLM STAT- MEDLINE DCOM- 20240112 LR - 20240113 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 16 IP - 1 DP - 2023 Dec 24 TI - Oleuropein-Rich Jasminum Grandiflorum Flower Extract Regulates the LKB1-PGC-1alpha Axis Related to the Attenuation of Hepatocellular Lipid Dysmetabolism. LID - 10.3390/nu16010058 [doi] LID - 58 AB - Diets() rich in fat are a major() cause() of metabolic disease(), and nutritional() food has been widely() used() to counteract the metabolic disorders such() as obesity() and fatty() liver(). The present study investigated the effects of oleuropein-enriched extract() from Jasminum grandiflorum L. flowers (OLE-JGF) in high-fat diet() (HFD)-fed mice and oleic acid() (OA)-treated AML-12 cells. Treatment() of HFD-fed mice with 0.6% OLE-JGF for 8 weeks significantly reduced body and liver() weights, as well as attenuating lipid dysmetabolism and hepatic steatosis. OLE-JGF administration() prominently suppressed the mRNA expressions() of monocyte chemoattractant protein()-1 (MCP-1) and cluster of differentiation 68 (CD68), and it also downregulated acetyl-CoA carboxylase (ACC) and fatty() acid() synthase (FAS) as well as sterol-regulatory-element()-binding protein() (SREBP-1c) in the liver(). Meanwhile, mitochondrial DNA and uncoupling protein() 2 (UCP2) were upregulated along with the increased expression() of mitochondrial biogenic promoters including liver() kinase B1 (LKB1), peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha), nuclear() factor()-erythroid-derived 2-like 2 (Nrf2), and mitochondrial transcription factor() A (Tfam), but did not change AMP-activated protein() kinase (AMPK) in liver(). The lipid droplets were decreased significantly after treatment() with 80 muM oleuropein for 24 h in OA-induced AML-12 cells. Furthermore, oleuropein significantly inhibited ACC mRNA expression() and upregulated LKB1, PGC-1alpha, and Tfam mRNA levels, as well as increasing the binding level of LKB1 to PGC-1alpha promoter in OA-induced cells. These findings indicate() that OLE-JGF reduces hepatic lipid deposition in HFD-fed mice, as well as the fact that OA-induced liver() cells may be partly() attributed to upregulation of the LKB1-PGC-1alpha axis, which mediates hepatic lipogenesis and mitochondrial biogenesis. Our study provides a scientific() basis() for the benefits and potential() use() of the J. grandiflorum flower as a food supplement() for the prevention() and treatment() of metabolic disease(). FAU - Hou, Yajun AU - Hou Y AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China. AD - International Healthcare Innovation Institute (Jiangmen), Jiangmen 529040, China. FAU - Zhao, Xuan AU - Zhao X AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China. FAU - Wang, Yalin AU - Wang Y AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China. FAU - Li, Yapeng AU - Li Y AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China. FAU - Chen, Caihong AU - Chen C AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China. FAU - Zhou, Xiu AU - Zhou X AUID- ORCID: 0000-0002-5495-2803 AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China. FAU - Jin, Jingwei AU - Jin J AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China. AD - International Healthcare Innovation Institute (Jiangmen), Jiangmen 529040, China. FAU - Ye, Jiming AU - Ye J AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China. FAU - Li, Dongli AU - Li D AUID- ORCID: 0000-0001-9955-2304 AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China. AD - International Healthcare Innovation Institute (Jiangmen), Jiangmen 529040, China. FAU - Gan, Lishe AU - Gan L AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China. AD - International Healthcare Innovation Institute (Jiangmen), Jiangmen 529040, China. FAU - Wu, Rihui AU - Wu R AUID- ORCID: 0000-0001-5395-1166 AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China. AD - International Healthcare Innovation Institute (Jiangmen), Jiangmen 529040, China. LA - eng GR - 22077111/National Natural Science Foundation of China/ GR - 82204035/National Natural Science Foundation of China/ GR - 81901678/National Natural Science Foundation of China/ GR - 2020KZDZX1203/Department of Education of Guangdong Province/ GR - 2021ZDZX4041/Department of Education of Guangdong Province/ GR - 2022KQNCX094/Department of Education of Guangdong Province/ GR - 2022A0505020026/Guangdong and Macao cooperation project from Department of Science and Technology of Guangdong Province and Jiangmen Science and Technology Bureau/ GR - 2020XTTGZYC02/Fund of Zhejiang Provincial Department of Agriculture and Rural Affairs/ PT - Journal Article DEP - 20231224 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 2O4553545L (oleuropein) RN - EC 2.7.11.1 (Protein Serine-Threonine Kinases) RN - EC 2.7.11.31 (AMP-Activated Protein Kinases) RN - EC 6.4.1.2 (Acetyl-CoA Carboxylase) RN - 0 (RNA, Messenger) RN - 0 (Plant Extracts) RN - 0 (Lipids) RN - 0 (Iridoid Glucosides) SB - IM MH - Animals MH - Mice MH - *Jasminum MH - *Carcinoma, Hepatocellular MH - *Liver Neoplasms MH - Protein Serine-Threonine Kinases MH - *Fatty Liver/drug therapy MH - AMP-Activated Protein Kinases/genetics MH - Acetyl-CoA Carboxylase MH - *Metabolic Diseases MH - RNA, Messenger MH - Plant Extracts/pharmacology MH - Lipids MH - *Leukemia, Myeloid, Acute MH - *Iridoid Glucosides PMC - PMC10780778 OTO - NOTNLM OT - Jasminum grandiflorum OT - LKB1-PGC-1alpha axis OT - hepatic steatosis OT - oleuropein COIS- The authors declare no conflict of interest. EDAT- 2024/01/11 07:42 MHDA- 2024/01/12 06:42 PMCR- 2023/12/24 CRDT- 2024/01/11 01:08 PHST- 2023/11/01 00:00 [received] PHST- 2023/12/12 00:00 [revised] PHST- 2023/12/21 00:00 [accepted] PHST- 2024/01/12 06:42 [medline] PHST- 2024/01/11 07:42 [pubmed] PHST- 2024/01/11 01:08 [entrez] PHST- 2023/12/24 00:00 [pmc-release] AID - nu16010058 [pii] AID - nutrients-16-00058 [pii] AID - 10.3390/nu16010058 [doi] PST - epublish SO - Nutrients. 2023 Dec 24;16(1):58. doi: 10.3390/nu16010058.