PMID- 38202671
OWN - NLM
STAT- MEDLINE
DCOM- 20240112
LR  - 20240116
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 29
IP  - 1
DP  - 2023 Dec 22
TI  - Main Colonic Metabolites from Coffee Chlorogenic Acid May Counteract Tumor 
      Necrosis Factor-alpha-Induced Inflammation and Oxidative Stress in 3T3-L1 Cells.
LID - 10.3390/molecules29010088 [doi]
LID - 88
AB  - Obesity is coupled with an altered redox state and low-level inflammation. 
      Oxidative stress may increase pre-adipocyte proliferation, adipocyte 
      differentiation and mature adipocyte size. Regarding inflammation, the 
      dysregulation of cytokine production by adipose tissue takes place in obesity, 
      which is promoted by oxidative stress. Polyphenols may exert a positive effect on 
      obesity, not only by modulating the redox state, but also due to their 
      anti-inflammatory activity. Coffee, which is one of the most consumed beverages, 
      is very rich in phenolic compounds. Bioavailability studies on coffee phenols 
      have shown that the most abundant group of metabolites in plasma and urine are 
      dihydrocaffeic (DHCA), dihydroferulic (DHFA), and hydroxyhippuric (HHA) acids, 
      the three acids of colonic origin. To better understand the antioxidant and 
      anti-inflammatory properties of DHCA, DHFA, and HHA, an inflammation/oxidation 
      model was set up in the pre-adipocyte 3T3-L1 cell line using tumor necrosis 
      factor-alpha (TNF-alpha). After the exposure of 3T3-L1 cells to 0.5, 1, 5, and 10 microM of 
      TNF-alpha at different times, the cell viability, interleukin (IL)-6 secretion, and 
      the production of reactive oxygen species (ROS) and glutathione (GSH) were 
      determined. Using the TNF-alpha prooxidant and proinflammatory conditions established 
      (10 microM, 24 h), it was observed that the physiological concentrations (0.5, 1, 5, 
      and 10 microM) of DHCA, DHFA, and HHA induced dose-dependent antioxidant effects 
      according to the ROS, GSH, and antioxidant enzyme (glutathione peroxidase) 
      results. In addition, reductions in the IL-1beta, IL-6, and monocyte chemoattractant 
      protein-1 (MCP-1) concentrations were observed to different extents depending on 
      the metabolite (DHFA, HHA, or DHCA) and the concentration used. In conclusion, 
      the main colonic metabolites from coffee chlorogenic acids may counteract 
      TNF-alpha-induced inflammation and oxidative stress in the 3T3-L1 cell line, and 
      thus, they present antiobesity potential.
FAU - Goya, Luis
AU  - Goya L
AUID- ORCID: 0000-0001-9449-6200
AD  - Department of Metabolism and Nutrition, Institute of Food Science, Technology and 
      Nutrition (ICTAN-CSIC), Spanish National Research Council (CSIC), Jose Antonio 
      Novais 6, 28040 Madrid, Spain.
FAU - Sanchez-Medina, Andrea
AU  - Sanchez-Medina A
AUID- ORCID: 0000-0002-6284-1716
AD  - Department of Metabolism and Nutrition, Institute of Food Science, Technology and 
      Nutrition (ICTAN-CSIC), Spanish National Research Council (CSIC), Jose Antonio 
      Novais 6, 28040 Madrid, Spain.
AD  - Department of Nutrition and Food Science, Faculty of Pharmacy, Universidad 
      Complutense de Madrid (UCM), Plaza de Ramon y Cajal, s/n, 28040 Madrid, Spain.
FAU - Redondo-Puente, Monica
AU  - Redondo-Puente M
AUID- ORCID: 0000-0002-3222-9489
AD  - Department of Metabolism and Nutrition, Institute of Food Science, Technology and 
      Nutrition (ICTAN-CSIC), Spanish National Research Council (CSIC), Jose Antonio 
      Novais 6, 28040 Madrid, Spain.
FAU - Dupak, Rudolf
AU  - Dupak R
AUID- ORCID: 0000-0002-9741-7771
AD  - Institute of Applied Biology, Faculty of Biotechnology and Food Sciences, Slovak 
      University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, 
      Slovakia.
FAU - Bravo, Laura
AU  - Bravo L
AUID- ORCID: 0000-0002-7312-8641
AD  - Department of Metabolism and Nutrition, Institute of Food Science, Technology and 
      Nutrition (ICTAN-CSIC), Spanish National Research Council (CSIC), Jose Antonio 
      Novais 6, 28040 Madrid, Spain.
FAU - Sarria, Beatriz
AU  - Sarria B
AUID- ORCID: 0000-0003-0614-4606
AD  - Department of Metabolism and Nutrition, Institute of Food Science, Technology and 
      Nutrition (ICTAN-CSIC), Spanish National Research Council (CSIC), Jose Antonio 
      Novais 6, 28040 Madrid, Spain.
AD  - Department of Nutrition and Food Science, Faculty of Pharmacy, Universidad 
      Complutense de Madrid (UCM), Plaza de Ramon y Cajal, s/n, 28040 Madrid, Spain.
LA  - eng
GR  - CSIC, Ref. 202170I026/Spanish National Research Council/
PT  - Journal Article
DEP - 20231222
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Coffee)
RN  - 318ADP12RI (Chlorogenic Acid)
RN  - 0 (Antioxidants)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Anti-Inflammatory Agents)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Coffee
MH  - *Chlorogenic Acid/pharmacology
MH  - Antioxidants/pharmacology
MH  - Tumor Necrosis Factor-alpha
MH  - Reactive Oxygen Species
MH  - 3T3-L1 Cells
MH  - Oxidative Stress
MH  - Inflammation/chemically induced/drug therapy
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Obesity
PMC - PMC10779949
OTO - NOTNLM
OT  - 3T3-L1
OT  - IL-1beta
OT  - IL-6
OT  - MCP-1
OT  - adipocytes
OT  - coffee metabolites
OT  - dihydrocaffeic acid
OT  - dihydroferulic acid
OT  - hydroxyhippuric acid
OT  - inflammation
OT  - obesity
OT  - oxidative stress
COIS- The authors declare no conflict of interest.
EDAT- 2024/01/11 07:42
MHDA- 2024/01/12 06:43
PMCR- 2023/12/22
CRDT- 2024/01/11 01:13
PHST- 2023/10/30 00:00 [received]
PHST- 2023/12/17 00:00 [revised]
PHST- 2023/12/19 00:00 [accepted]
PHST- 2024/01/12 06:43 [medline]
PHST- 2024/01/11 07:42 [pubmed]
PHST- 2024/01/11 01:13 [entrez]
PHST- 2023/12/22 00:00 [pmc-release]
AID - molecules29010088 [pii]
AID - molecules-29-00088 [pii]
AID - 10.3390/molecules29010088 [doi]
PST - epublish
SO  - Molecules. 2023 Dec 22;29(1):88. doi: 10.3390/molecules29010088.