PMID- 38204243
OWN - NLM
STAT- Publisher
LR  - 20240111
IS  - 2212-3946 (Electronic)
IS  - 1574-888X (Linking)
DP  - 2024 Jan 9
TI  - Bioinformatics-based Study on the Effects of Umbilical Cord Mesenchymal Stem 
      Cells on the Aging Retina.
LID - 10.2174/011574888X277276231215110316 [doi]
AB  - BACKGROUND: Retinal aging is one of the common public health problems caused by 
      population aging and has become an important cause of acquired vision loss in 
      adults. The aim of this study was to determine the role of human umbilical cord 
      mesenchymal stem cells (hUCMSCs) in delaying retinal ganglion cell (RGC) aging 
      and part of the network of molecular mechanisms involved. METHODS: A retinal 
      ganglion cell senescence model was established in vitro and treated with UCMSC. 
      Successful establishment of the senescence system was demonstrated using beta- 
      galactosidase staining. The ameliorative effect of MSC on senescence was 
      demonstrated using CCK8 cell viability and Annexin V-PI apoptosis staining. The 
      relevant targets of RGC, MSC, and senescence were mainly obtained by searching 
      the GeneCards database. The protein interaction network among the relevant 
      targets was constructed using the String database and Cytoscape, and 10 key 
      target genes were calculated based on the MCC algorithm, based on which Gene 
      ontologies (GO) enrichment and the Kyoto Encyclopedia of Genes and Genomes (KEGG) 
      enrichment were performed. Changes in relevant target genes were detected using 
      real-time fluorescence quantitative PCR and the mechanism of action of UCMSC was 
      determined by RNA interference. RESULTS: beta-galactosidase staining showed that 
      UCMSC significantly reduced the positive results of RGC. The retinal aging 
      process was alleviated. The bioinformatics screen yielded 201 shared genes. 10 
      key genes were selected by the MCC algorithm, including vascular endothelial 
      growth factor A (VEGFA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 
      albumin (ALB), interleukin- 6 (IL6), tumor necrosis factor (TNF), tumor protein 
      P53 (TP53), insulin (INS), matrix metalloproteinase 9 (MMP9), epidermal growth 
      factor (EGF), interleukin-1beta (IL1B), and enrichment to related transferase 
      activity and kinase activity regulated biological processes involved in oxidative 
      stress and inflammation related pathways. In addition, PCR results showed that 
      all the above molecules were altered in expression after UCMSC involvement. 
      CONCLUSION: This experiment demonstrated the role of UCMSC in delaying retinal 
      ganglion cell senescence and further elucidated that UCMSC may be associated with 
      the activation of VEGFA, TP53, ALB, GAPDH, IL6, IL1B, MMP9 genes and the 
      inhibition of INS, EGF, and TNF in delaying retinal senescence.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Shi, Ya-Hui
AU  - Shi YH
AD  - Liaoning Key Laboratory of Diabetic Cognitive and Perceptive Dysfunction, Jinzhou 
      Medical University, Jinzhou, 121000, China.
FAU - Li, Jun-Qi
AU  - Li JQ
AD  - Liaoning Key Laboratory of Diabetic Cognitive and Perceptive Dysfunction, Jinzhou 
      Medical University, Jinzhou, 121000, China.
FAU - Xu, Min
AU  - Xu M
AD  - Liaoning Key Laboratory of Diabetic Cognitive and Perceptive Dysfunction, Jinzhou 
      Medical University, Jinzhou, 121000, China.
FAU - Wang, Yu-Ying
AU  - Wang YY
AD  - Liaoning Key Laboratory of Diabetic Cognitive and Perceptive Dysfunction, Jinzhou 
      Medical University, Jinzhou, 121000, China.
FAU - Wang, Ting-Hua
AU  - Wang TH
AD  - Liaoning Key Laboratory of Diabetic Cognitive and Perceptive Dysfunction, Jinzhou 
      Medical University, Jinzhou, 121000, China.
AD  - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, China.
FAU - Zuo, Zhong-Fu
AU  - Zuo ZF
AD  - Liaoning Key Laboratory of Diabetic Cognitive and Perceptive Dysfunction, Jinzhou 
      Medical University, Jinzhou, 121000, China.
AD  - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, China.
FAU - Liu, Xue-Zheng
AU  - Liu XZ
AD  - Liaoning Key Laboratory of Diabetic Cognitive and Perceptive Dysfunction, Jinzhou 
      Medical University, Jinzhou, 121000, China.
AD  - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, China.
LA  - eng
PT  - Journal Article
DEP - 20240109
PL  - United Arab Emirates
TA  - Curr Stem Cell Res Ther
JT  - Current stem cell research & therapy
JID - 101272517
SB  - IM
OTO - NOTNLM
OT  - Retinal ganglion cells
OT  - aging
OT  - apoptosis
OT  - bioinformatics analysis
OT  - oxidative stress.
OT  - umbilical cord mesenchymal stem cells
EDAT- 2024/01/11 07:43
MHDA- 2024/01/11 07:43
CRDT- 2024/01/11 02:26
PHST- 2023/09/02 00:00 [received]
PHST- 2023/11/09 00:00 [revised]
PHST- 2023/11/16 00:00 [accepted]
PHST- 2024/01/11 07:43 [medline]
PHST- 2024/01/11 07:43 [pubmed]
PHST- 2024/01/11 02:26 [entrez]
AID - CSCR-EPUB-137163 [pii]
AID - 10.2174/011574888X277276231215110316 [doi]
PST - aheadofprint
SO  - Curr Stem Cell Res Ther. 2024 Jan 9. doi: 10.2174/011574888X277276231215110316.