PMID- 38214142
OWN - NLM
STAT- MEDLINE
DCOM- 20240412
LR  - 20240412
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 239
IP  - 4
DP  - 2024 Apr
TI  - Single-cell transcriptome analysis reveals tumoral microenvironment 
      heterogenicity and hypervascularization in human carotid body tumor.
PG  - e31175
LID - 10.1002/jcp.31175 [doi]
AB  - Carotid body tumor (CBT) is a rare neck tumor located at the adventitia of the 
      common carotid artery bifurcation. The prominent pathological features of CBT are 
      high vascularization and abnormal proliferation. However, single-cell 
      transcriptome analysis of the microenvironment composition and molecular 
      complexity in CBT has yet to be performed. In this study, we performed 
      single-cell RNA sequencing (scRNA-seq) analysis on human CBT to define the cells 
      that contribute to hypervascularization and chronic hyperplasia. Unbiased 
      clustering analysis of transcriptional profiles identified 16 distinct cell 
      populations including endothelial cells (ECs), smooth muscle cells (SMCs), neuron 
      cells, macrophage cells, neutrophil cells, and T cells. Within the ECs 
      population, we defined subsets with angiogenic capacity plus clear signs of later 
      endothelial progenitor cells (EPCs) to normal ECs. Two populations of macrophages 
      were detectable in CBT, macrophage1 showed enrichment in hypoxia-inducible 
      factor-1 (HIF-1) and as well as an early EPCs cell-like population expressing 
      CD14 and vascular endothelial growth factor. In addition to HIF-1-related 
      transcriptional protein expression, macrophages1 also display a 
      neovasculogenesis-promoting phenotype. SMCs included three populations showing 
      platelet-derived growth factor receptor beta and vimentin expression, indicative 
      of a cancer-associated fibroblast phenotype. Finally, we identified three types 
      of neuronal cells, including chief cells and sustentacular cells, and elucidated 
      their distinct roles in the pathogenesis of CBT and abnormal proliferation of 
      tumors. Overall, our study provided the first comprehensive characterization of 
      the transcriptional landscape of CBT at scRNA-seq profiles, providing novel 
      insights into the mechanisms underlying its formation.
CI  - (c) 2024 Wiley Periodicals LLC.
FAU - Cai, Gaopo
AU  - Cai G
AUID- ORCID: 0009-0009-2787-4131
AD  - Department of Endovascular Surgery, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Hua, Zhaohui
AU  - Hua Z
AD  - Department of Endovascular Surgery, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Zhang, Linfeng
AU  - Zhang L
AD  - Department of Endovascular Surgery, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Chen, Yutian
AU  - Chen Y
AD  - Department of Endovascular Surgery, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Li, Xu
AU  - Li X
AD  - Department of Endovascular Surgery, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Ma, Ke
AU  - Ma K
AD  - Department of Endovascular Surgery, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Xia, Zongping
AU  - Xia Z
AD  - Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Li, Zhen
AU  - Li Z
AD  - Department of Endovascular Surgery, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, China.
LA  - eng
GR  - 81873527/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20240112
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Humans
MH  - Single-Cell Gene Expression Analysis
MH  - *Carotid Body Tumor/pathology
MH  - Vascular Endothelial Growth Factor A
MH  - Carotid Arteries/pathology
MH  - *Endothelial Progenitor Cells
MH  - Transcriptome/genetics
MH  - Tumor Microenvironment/genetics
MH  - Single-Cell Analysis
OTO - NOTNLM
OT  - carotid body tumor
OT  - hypervascularization
OT  - microenvironment
OT  - single cell sequencing
EDAT- 2024/01/12 06:42
MHDA- 2024/04/12 06:44
CRDT- 2024/01/12 04:44
PHST- 2023/11/07 00:00 [revised]
PHST- 2023/07/18 00:00 [received]
PHST- 2023/12/07 00:00 [accepted]
PHST- 2024/04/12 06:44 [medline]
PHST- 2024/01/12 06:42 [pubmed]
PHST- 2024/01/12 04:44 [entrez]
AID - 10.1002/jcp.31175 [doi]
PST - ppublish
SO  - J Cell Physiol. 2024 Apr;239(4):e31175. doi: 10.1002/jcp.31175. Epub 2024 Jan 12.