PMID- 38216808
OWN - NLM
STAT- Publisher
LR  - 20240112
IS  - 1559-0283 (Electronic)
IS  - 1085-9195 (Linking)
DP  - 2024 Jan 13
TI  - Exosomes Derived from hucMSCs Primed with IFN-gamma Suppress the NF-kappaB Signal Pathway 
      in LPS-Induced ALI by Modulating the miR-199b-5p/AFTPH Axis.
LID - 10.1007/s12013-023-01208-2 [doi]
AB  - Exosomes (exos) are primarily responsible for the process of mesenchymal stem 
      cells (MSCs) treatment for acute lung injury (ALI), but the mechanism remains 
      unclear, particularly in altered microenvironment. Therefore, this study aimed to 
      investigate the potential mechanism of exos derived from human umbilical cord 
      mesenchymal stem cells (hucMSCs) primed with interferon-gamma (IFN-gamma) on ALI and 
      to propose a promising and cell-free strategy. This study extracted exos from 
      hucMSCs supernatant primed and unprimed with IFN-gamma marked with IFN-gamma-exos and 
      CON-exos, which were identified and traced. IFN-gamma-exos administration to ALI 
      models suppressed the NF-kappaB signaling pathway compared to CON-exos, which were 
      quantified through western blot and immunohistochemical staining. Reverse 
      transcription-quantitative polymerase chain reaction validated miR-199b-5p 
      expression in the IFN-gamma-exos and CON-exos treatment groups. Data analysis, a 
      dual-luciferase reporter assay, and cell transfection were conducted to 
      investigate the target binding between miR-199b-5p and Aftiphilin (AFTPH), with 
      AFTPH expression analyzed via cell immunofluorescence and western blot. 
      Co-immunoprecipitation was conducted for the interaction between AFTPH and NF-kappaB 
      p65. The result revealed that miR-199b-5p was down-regulated in the IFN-gamma-exos 
      treatment group, which had a target binding site with AFTPH, and an interaction 
      with NF-kappaB p65. Consequently, IFN-gamma-exos inhibited the NF-kappaB signaling pathway in 
      ALI in vitro and in vivo through the miR-199b-5p/AFTPH axis. Our results 
      demonstrated new directions of novel and targeted treatment for ALI.
CI  - (c) 2024. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Wang, Chun
AU  - Wang C
AD  - Kunming Medical University, Kunming, Yunnan, China.
AD  - Department of Emergency Intensive Care Unit, Second Affiliated Hospital of 
      Kunming Medical University, Kunming, Yunnan, China.
FAU - Yang, Yiran
AU  - Yang Y
AD  - Kunming Medical University, Kunming, Yunnan, China.
FAU - Jiang, Chen
AU  - Jiang C
AD  - Kunming Medical University, Kunming, Yunnan, China.
FAU - Xi, Cheng
AU  - Xi C
AD  - Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming 
      Medical University, Kunming, Yunnan, China.
FAU - Yin, Yunxiang
AU  - Yin Y
AD  - Department of Emergency Intensive Care Unit, Second Affiliated Hospital of 
      Kunming Medical University, Kunming, Yunnan, China.
FAU - Wu, Haiying
AU  - Wu H
AD  - Department of Emergency, First Affiliated Hospital of Kunming Medical University, 
      Kunming, Yunnan, China. wuhaiying@kmmu.edu.cn.
FAU - Qian, Chuanyun
AU  - Qian C
AD  - Department of Emergency, First Affiliated Hospital of Kunming Medical University, 
      Kunming, Yunnan, China. qianchuanyun@kmmu.edu.cn.
LA  - eng
GR  - 81960817/the National Natural Science Foundation of China/
GR  - 82260384/the National Natural Science Foundation of China/
GR  - 2020-1 - H-011/the Kunming Science and Technology Bureau/
PT  - Journal Article
DEP - 20240113
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
SB  - IM
OTO - NOTNLM
OT  - AFTPH
OT  - Acute lung injury
OT  - Exosomes
OT  - Human umbilical cord mesenchymal stem cells
OT  - Interferon-gamma
OT  - miR-199b-5p
EDAT- 2024/01/13 00:42
MHDA- 2024/01/13 00:42
CRDT- 2024/01/12 23:29
PHST- 2023/08/03 00:00 [received]
PHST- 2023/11/30 00:00 [accepted]
PHST- 2024/01/13 00:42 [medline]
PHST- 2024/01/13 00:42 [pubmed]
PHST- 2024/01/12 23:29 [entrez]
AID - 10.1007/s12013-023-01208-2 [pii]
AID - 10.1007/s12013-023-01208-2 [doi]
PST - aheadofprint
SO  - Cell Biochem Biophys. 2024 Jan 13. doi: 10.1007/s12013-023-01208-2.