PMID- 38217740
OWN - NLM
STAT- Publisher
LR  - 20240113
IS  - 1615-6102 (Electronic)
IS  - 0033-183X (Linking)
DP  - 2024 Jan 13
TI  - Structure, ultrastructure and cation accumulation in quinoa epidermal bladder 
      cell complex under high saline stress.
LID - 10.1007/s00709-023-01922-x [doi]
AB  - Quinoa is a facultative halophyte with excellent tolerance to salinity. In this 
      study, the epidermal bladder cell complex (EBCc) of quinoa leaves was studied to 
      determine their cellular characteristics and involvement in salt tolerance. We 
      used light microscopy, confocal RAMAN microscopy, confocal fluorescence 
      microscopy, transmission electron microscopy, and environmental scanning electron 
      microscopy complemented by energy dispersive X-ray analysis. Ionic content was 
      quantified with flame atomic absorption spectroscopy and with flame emission 
      photometry. Results show that: (i) the number of EBCcs remains constant but their 
      density and area vary with leaf age; (ii) stalk cells store lipids and exhibit 
      thick walls, bladder cells present carotenes in small vesicles, oxalate crystals 
      in vacuoles and lignin in their walls and both stalk and bladder cells have 
      cuticles that differ in wax and cutin content; (iii) chloroplasts containing 
      starch can be found on both stalk and bladder cells, and the latter also presents 
      grana; (iv) plasmodesmata are observed between the stalk cell and the bladder 
      cell, and between the epidermal cell and the stalk cell, and ectodesmata-like 
      structures are observed on the bladder cell. Under high salinity conditions, (v) 
      there is a clear tendency to accumulate greater amounts of K(+) with respect to 
      Na(+) in the bladder cell; (vi) stalk cells accumulate similar amounts of K(+) 
      and Na(+); (vii) Na(+) accumulates mainly in the medullary parenchyma of the 
      stem. These results add knowledge about the structure, content, and role of EBCc 
      under salt stress, and surprisingly present the parenchyma of the stem as the 
      main area of Na(+) accumulation.
CI  - (c) 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, 
      part of Springer Nature.
FAU - Palacios, Maria Belen
AU  - Palacios MB
AD  - Departamento de Biodiversidad y Biologia Experimental, Facultad de Ciencias 
      Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
AD  - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina.
FAU - Rizzo, Axel Joel
AU  - Rizzo AJ
AD  - Departamento de Biodiversidad y Biologia Experimental, Facultad de Ciencias 
      Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
AD  - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina.
FAU - Heredia, Tatiana Belen
AU  - Heredia TB
AD  - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina.
AD  - Estacion Experimental Agropecuaria San Juan, Instituto Nacional de Tecnologia 
      Agropecuaria (INTA), San Juan, Argentina.
FAU - Roqueiro, Gonzalo
AU  - Roqueiro G
AD  - Estacion Experimental Agropecuaria San Juan, Instituto Nacional de Tecnologia 
      Agropecuaria (INTA), San Juan, Argentina.
FAU - Maldonado, Sara
AU  - Maldonado S
AD  - Departamento de Biodiversidad y Biologia Experimental, Facultad de Ciencias 
      Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
AD  - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina.
FAU - Murgida, Daniel Horacio
AU  - Murgida DH
AD  - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina.
AD  - Departamento de Quimica Inorganica, Analitica y Quimica Fisica and INQUIMAE, 
      Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos 
      Aires, Argentina.
FAU - Burrieza, Hernan Pablo
AU  - Burrieza HP
AUID- ORCID: 0000-0001-7795-613X
AD  - Departamento de Biodiversidad y Biologia Experimental, Facultad de Ciencias 
      Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina. 
      hernan@bg.fcen.uba.ar.
AD  - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos 
      Aires, Argentina. hernan@bg.fcen.uba.ar.
LA  - eng
GR  - 20020170200265BA/Secretaria de Ciencia y Tecnica, Universidad de Buenos Aires/
PT  - Journal Article
DEP - 20240113
PL  - Austria
TA  - Protoplasma
JT  - Protoplasma
JID - 9806853
SB  - IM
OTO - NOTNLM
OT  - Chenopodium quinoa
OT  - Epidermal bladder cell complex
OT  - Potassium
OT  - Salt stress
OT  - Sodium
OT  - Structural biology
EDAT- 2024/01/13 17:44
MHDA- 2024/01/13 17:44
CRDT- 2024/01/13 11:15
PHST- 2023/10/05 00:00 [received]
PHST- 2023/12/21 00:00 [accepted]
PHST- 2024/01/13 17:44 [medline]
PHST- 2024/01/13 17:44 [pubmed]
PHST- 2024/01/13 11:15 [entrez]
AID - 10.1007/s00709-023-01922-x [pii]
AID - 10.1007/s00709-023-01922-x [doi]
PST - aheadofprint
SO  - Protoplasma. 2024 Jan 13. doi: 10.1007/s00709-023-01922-x.