PMID- 38219725 OWN - NLM STAT- MEDLINE DCOM- 20240408 LR - 20240408 IS - 1423-0135 (Electronic) IS - 1018-1172 (Linking) VI - 61 IP - 2 DP - 2024 TI - Preserving Endothelial Integrity in Human Saphenous Veins during Preparation for Coronary Bypass Surgery. PG - 68-76 LID - 10.1159/000535843 [doi] AB - INTRODUCTION: While multiple factors influence coronary artery bypass graft (CABG) success rates, preserving saphenous vein endothelium during surgery may improve patency. Standard preparations include saphenous vein preparation in heparinized saline (saline) which can result in endothelial loss and damage. Here, we investigated the impact of preparing saphenous graft vessels in heparinized patient blood (blood) versus saline. METHODS: Saphenous vein tissues from a total of 23 patients undergoing CABG were split into 2 groups (1) saline and (2) heparinized patient blood. Excess tissue was fixed for analysis immediately following surgery. Level of endothelial coverage, oxidative stress marker 4-hydroxynonenal (4HNE), and oxidative stress protective marker nuclear factor erythroid 2-related factor 2 (NRF2) were evaluated. RESULTS: In saline patient veins, histological analysis revealed a limited luminal layer, suggesting a loss of endothelial cells (ECs). Immunofluorescent staining of EC markers vascular endothelial cadherin (VE-cadherin) and endothelial nitric oxide identified a significant improvement in EC coverage in the blood versus saline groups. Although both treatment groups expressed 4HNE to similar levels, EC blood samples expressed higher levels of NRF2. CONCLUSION: Our data indicate that use of heparinized patient blood helps preserve the endothelium and promotes vein graft health. This has the potential to improve long-term outcomes in patients. CI - (c) 2024 S. Karger AG, Basel. FAU - Sedovy, Meghan W AU - Sedovy MW AD - The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA. AD - Translational Biology, Medicine, and Health Graduate Program, Virginia Tech, Blacksburg, Virginia, USA. FAU - Leng, Xinyan AU - Leng X AD - The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA. FAU - Iqbal, Farwah AU - Iqbal F AD - The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA. AD - Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA. FAU - Renton, Mark C AU - Renton MC AD - The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA. FAU - Leaf, Melissa AU - Leaf M AD - The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA. AD - Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA. FAU - Roberts, Kailynn AU - Roberts K AD - The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA. FAU - Malek, Arya AU - Malek A AD - The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA. FAU - Arnold, W Scott AU - Arnold WS AD - Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA. AD - Carilion Cardiothoracic Surgery, Cardiovascular Institute, Carilion Clinic, Roanoke, Virginia, USA. FAU - Wyatt, David A AU - Wyatt DA AD - Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA. AD - Carilion Cardiothoracic Surgery, Cardiovascular Institute, Carilion Clinic, Roanoke, Virginia, USA. FAU - Choate, Cynthia W AU - Choate CW AD - Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA. AD - Carilion Cardiothoracic Surgery, Cardiovascular Institute, Carilion Clinic, Roanoke, Virginia, USA. FAU - Rowe, Joseph F AU - Rowe JF AD - Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA. AD - Carilion Cardiothoracic Surgery, Cardiovascular Institute, Carilion Clinic, Roanoke, Virginia, USA. FAU - Baker, Joseph W AU - Baker JW AD - Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA. AD - Carilion Cardiothoracic Surgery, Cardiovascular Institute, Carilion Clinic, Roanoke, Virginia, USA. FAU - Johnstone, Scott R AU - Johnstone SR AD - The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA. AD - Department of Biological Sciences, Virginia Tech, Blacksburg, Virginia, USA. FAU - Joseph, Mark AU - Joseph M AD - The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA. AD - Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA. AD - Carilion Cardiothoracic Surgery, Cardiovascular Institute, Carilion Clinic, Roanoke, Virginia, USA. LA - eng PT - Journal Article DEP - 20240112 PL - Switzerland TA - J Vasc Res JT - Journal of vascular research JID - 9206092 RN - 0 (NF-E2-Related Factor 2) SB - IM MH - Humans MH - *Saphenous Vein/pathology MH - *Endothelial Cells MH - NF-E2-Related Factor 2 MH - Endothelium, Vascular/pathology MH - Coronary Artery Bypass/adverse effects OTO - NOTNLM OT - Coronary artery bypass graft OT - Endothelial cells OT - Saphenous vein OT - Smooth muscle cell EDAT- 2024/01/15 00:42 MHDA- 2024/04/08 06:42 CRDT- 2024/01/14 18:28 PHST- 2023/11/01 00:00 [received] PHST- 2023/12/12 00:00 [accepted] PHST- 2024/04/08 06:42 [medline] PHST- 2024/01/15 00:42 [pubmed] PHST- 2024/01/14 18:28 [entrez] AID - 000535843 [pii] AID - 10.1159/000535843 [doi] PST - ppublish SO - J Vasc Res. 2024;61(2):68-76. doi: 10.1159/000535843. Epub 2024 Jan 12.