PMID- 38222561
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240116
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 9
IP  - 1
DP  - 2024 Jan 9
TI  - Engineering Temperature-Responsive Polymer Nanoparticles that Load and Release 
      Paclitaxel, a Low-Molecular-Weight Anticancer Drug.
PG  - 1011-1019
LID - 10.1021/acsomega.3c07226 [doi]
AB  - Poly(N-isopropylacrylamide) (pNIPAm) undergoes a hydrophilicity/hydrophobicity 
      change around its lower critical solution temperature (LCST). Therefore, 
      pNIPAm-based polymer nanoparticles (NPs) shrink above their LCST and swell below 
      their LCST. Although temperature responsiveness is an important characteristic of 
      synthetic polymers in drug and gene delivery, few studies have investigated the 
      temperature-responsive catch and release of low-molecular-weight drugs (LMWDs) as 
      their affinity to the target changes. Since LMWDs have only a few functional 
      groups, preparation of NPs with high affinity for LMWDs is hard compared with 
      that for peptides and proteins. However, LMWDs such as anticancer drugs often 
      have a stronger effect than peptides and proteins. Therefore, the development of 
      NPs that can load and release LMWDs is needed for drug delivery. Here, we 
      engineered pNIPAm-based NPs that capture paclitaxel (PTX), an anticancer LMWD 
      that inhibits microtubules, above their LCST and release it below their LCST. The 
      swelling transition of the NPs depended on their hydrophobic monomer structure. 
      NPs with swelling ratios (=NP size at 25  degrees C/NP size at 37  degrees C) exceeding 1.90 
      released captured PTX when cooled to below their LCST by changing the affinity 
      for PTX. On the other hand, NPs with a swelling ratio of only 1.14 released 
      melittin. Therefore, optimizing the functional monomers of temperature-responsive 
      NPs is essential for the catch and release of the target in a 
      temperature-dependent manner. These results can guide the design of 
      stimuli-responsive polymers that catch and release their target molecules.
CI  - (c) 2023 The Authors. Published by American Chemical Society.
FAU - Koide, Hiroyuki
AU  - Koide H
AUID- ORCID: 0000-0003-1763-6593
AD  - Department of Medical Biochemistry, University of Shizuoka School of 
      Pharmaceutical Sciences, 52-1 Yada, Suruga-ku, Shizuoka, Shizuoka 422-8526, 
      Japan.
FAU - Yamaguchi, Kazuma
AU  - Yamaguchi K
AD  - Department of Medical Biochemistry, University of Shizuoka School of 
      Pharmaceutical Sciences, 52-1 Yada, Suruga-ku, Shizuoka, Shizuoka 422-8526, 
      Japan.
FAU - Sato, Keijiro
AU  - Sato K
AD  - Department of Medical Biochemistry, University of Shizuoka School of 
      Pharmaceutical Sciences, 52-1 Yada, Suruga-ku, Shizuoka, Shizuoka 422-8526, 
      Japan.
FAU - Aoshima, Maki
AU  - Aoshima M
AD  - Department of Medical Biochemistry, University of Shizuoka School of 
      Pharmaceutical Sciences, 52-1 Yada, Suruga-ku, Shizuoka, Shizuoka 422-8526, 
      Japan.
FAU - Kanata, Shoko
AU  - Kanata S
AD  - Department of Medical Biochemistry, University of Shizuoka School of 
      Pharmaceutical Sciences, 52-1 Yada, Suruga-ku, Shizuoka, Shizuoka 422-8526, 
      Japan.
FAU - Yonezawa, Sei
AU  - Yonezawa S
AD  - Department of Medical Biochemistry, University of Shizuoka School of 
      Pharmaceutical Sciences, 52-1 Yada, Suruga-ku, Shizuoka, Shizuoka 422-8526, 
      Japan.
FAU - Asai, Tomohiro
AU  - Asai T
AD  - Department of Medical Biochemistry, University of Shizuoka School of 
      Pharmaceutical Sciences, 52-1 Yada, Suruga-ku, Shizuoka, Shizuoka 422-8526, 
      Japan.
LA  - eng
PT  - Journal Article
DEP - 20231227
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC10785788
COIS- The authors declare no competing financial interest.
EDAT- 2024/01/15 06:42
MHDA- 2024/01/15 06:43
PMCR- 2023/12/27
CRDT- 2024/01/15 04:34
PHST- 2023/09/20 00:00 [received]
PHST- 2023/12/11 00:00 [revised]
PHST- 2023/12/13 00:00 [accepted]
PHST- 2024/01/15 06:43 [medline]
PHST- 2024/01/15 06:42 [pubmed]
PHST- 2024/01/15 04:34 [entrez]
PHST- 2023/12/27 00:00 [pmc-release]
AID - 10.1021/acsomega.3c07226 [doi]
PST - epublish
SO  - ACS Omega. 2023 Dec 27;9(1):1011-1019. doi: 10.1021/acsomega.3c07226. eCollection 
      2024 Jan 9.