PMID- 38222631
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240116
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 9
IP  - 1
DP  - 2024 Jan 9
TI  - Itaconic Acid-Based Organic-Polymer Monolithic Column for Hydrophilic Capillary 
      Electrochromatography and Its Application in Pharmaceutical Analysis.
PG  - 1554-1561
LID - 10.1021/acsomega.3c08031 [doi]
AB  - Itaconic acid is an excellent hydrophilic monomer owing to the dicarboxylic group 
      possessing strong polarity. This study reports on the preparation of a new 
      organic-polymer monolithic column poly(itaconic 
      acid-co-3-(acryloyloxy)-2-hydroxypropyl methacrylate) (poly(IA-co-AHM)) featuring 
      excellent hydrophilic chromatography ability and its application in 
      pharmaceutical analysis. The monolithic column was successfully synthesized by 
      using the monomer itaconic acid and the cross-linker AHM through an in situ 
      copolymerization method. Optical microscopy, scanning electron microscopy (SEM), 
      and Fourier transform infrared spectroscopy (FTIR) were employed for the 
      characterization of the poly(IA-co-AHM) monolithic column, and all of these 
      demonstrated that the prepared itaconic acid-based monolithic column exhibited 
      satisfactory permeability and a homogeneous porous structure. Owing to the 
      carboxylic groups of itaconic acid, a cathodic electroosmotic flow (EOF) was 
      generated on the itaconic acid-based monolithic column among the pH ranges of the 
      mobile phase from 4.0 to 9.0. Depending on the powerful hydrophilic interactions, 
      different kinds of polar substances, including thioureas, nucleoside drugs, 
      sulfonamides, and polypeptides, were separated efficiently by the itaconic 
      acid-based monoliths poly(IA-co-AHM). The separations of polar compounds were 
      successfully realized, even at a lower level of 50% acetonitrile content on this 
      monolithic column. The highest column efficiencies corresponding to 
      N,N'-dimethylthiourea and idoxuridine were 102 720 and 124 267 N/m, respectively. 
      The poly(IA-co-AHM) monolithic column displayed excellent repeatability, whose 
      relative standard deviations (RSDs) of the retention time and peak area were both 
      lower than 5.0%. All experimental results demonstrated that the new itaconic 
      acid-functionalized monolithic column was greatly appropriate to separate the 
      polar compounds under the HILIC mode.
CI  - (c) 2023 The Authors. Published by American Chemical Society.
FAU - Mao, Zhenkun
AU  - Mao Z
AUID- ORCID: 0000-0002-6314-4090
AD  - Department of Pharmacy, Henan Provincial People's Hospital, Zhengzhou 450003, 
      Henan, China.
AD  - Department of Pharmacy, People's Hospital of Zhengzhou University, Zhengzhou 
      University, Zhengzhou 450003, Henan, China.
FAU - Chen, Jinxiu
AU  - Chen J
AD  - Department of Pharmacy, Henan Provincial People's Hospital, Zhengzhou 450003, 
      Henan, China.
AD  - Department of Pharmacy, People's Hospital of Zhengzhou University, Zhengzhou 
      University, Zhengzhou 450003, Henan, China.
FAU - Jiang, Dandan
AU  - Jiang D
AD  - Department of Pharmacy, Henan Provincial People's Hospital, Zhengzhou 450003, 
      Henan, China.
AD  - Department of Pharmacy, People's Hospital of Zhengzhou University, Zhengzhou 
      University, Zhengzhou 450003, Henan, China.
FAU - Zhao, Ningmin
AU  - Zhao N
AD  - Department of Pharmacy, Henan Provincial People's Hospital, Zhengzhou 450003, 
      Henan, China.
AD  - Department of Pharmacy, People's Hospital of Zhengzhou University, Zhengzhou 
      University, Zhengzhou 450003, Henan, China.
FAU - Qin, Yinhui
AU  - Qin Y
AD  - Department of Pharmacy, Henan Provincial People's Hospital, Zhengzhou 450003, 
      Henan, China.
AD  - Department of Pharmacy, People's Hospital of Zhengzhou University, Zhengzhou 
      University, Zhengzhou 450003, Henan, China.
FAU - Mao, Xiangju
AU  - Mao X
AD  - Zhengzhou Institute of Multipurpose Utilization of Mineral Resources, CAGS, 
      Zhengzhou 450006, China.
FAU - Fang, Fengqin
AU  - Fang F
AD  - Department of Pharmacy, Henan Provincial People's Hospital, Zhengzhou 450003, 
      Henan, China.
AD  - Department of Pharmacy, People's Hospital of Zhengzhou University, Zhengzhou 
      University, Zhengzhou 450003, Henan, China.
FAU - Ma, Peizhi
AU  - Ma P
AD  - Department of Pharmacy, Henan Provincial People's Hospital, Zhengzhou 450003, 
      Henan, China.
AD  - Department of Pharmacy, People's Hospital of Zhengzhou University, Zhengzhou 
      University, Zhengzhou 450003, Henan, China.
LA  - eng
PT  - Journal Article
DEP - 20231227
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC10785275
COIS- The authors declare no competing financial interest.
EDAT- 2024/01/15 06:42
MHDA- 2024/01/15 06:43
PMCR- 2023/12/27
CRDT- 2024/01/15 04:35
PHST- 2023/10/13 00:00 [received]
PHST- 2023/11/07 00:00 [revised]
PHST- 2023/12/11 00:00 [accepted]
PHST- 2024/01/15 06:43 [medline]
PHST- 2024/01/15 06:42 [pubmed]
PHST- 2024/01/15 04:35 [entrez]
PHST- 2023/12/27 00:00 [pmc-release]
AID - 10.1021/acsomega.3c08031 [doi]
PST - epublish
SO  - ACS Omega. 2023 Dec 27;9(1):1554-1561. doi: 10.1021/acsomega.3c08031. eCollection 
      2024 Jan 9.