PMID- 38224664
OWN - NLM
STAT- MEDLINE
DCOM- 20240202
LR  - 20240206
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 696
DP  - 2024 Feb 12
TI  - Epcam regulates intrahepatic bile duct reconstruction in zebrafish, providing a 
      potential model for primary cholangitis model.
PG  - 149512
LID - S0006-291X(24)00047-0 [pii]
LID - 10.1016/j.bbrc.2024.149512 [doi]
AB  - Epithelial cell adhesion molecules (EpCAMs) have been identified as surface 
      markers of proliferating ductal cells, which are referred to as liver progenitor 
      cells (LPCs), during liver regeneration and correspond to malignancies. These 
      cells can differentiate into hepatocytes and biliary epithelial cells (BECs) in 
      vitro. EpCAM-positive LPCs are involved in liver regeneration following severe 
      liver injury; however, the in vivo function of EpCAMs in the regenerating liver 
      remains unclear. In the present study, we used a zebrafish model of LPC-driven 
      liver regeneration to elucidate the function of EpCAMs in the regenerating liver 
      in vivo. Proliferating ductal cells were observed after severe hepatocyte loss in 
      the zebrafish model. Analyses of the liver size as well as hepatocyte and BEC 
      markers revealed successful conversion of LPCs to hepatocytes and BECs in epcam 
      mutants. Notably, epcam mutants exhibited severe defects in intrahepatic duct 
      maturation and bile acid secretion in regenerating hepatocytes, suggesting that 
      epcam plays a critical role in intrahepatic duct reconstruction during LPC-driven 
      liver regeneration. Our findings provide insights into human diseases involving 
      non-parenchymal cells, such as primary biliary cholangitis, by highlighting the 
      regulatory effect of epcam on intrahepatic duct reconstruction.
CI  - Copyright (c) 2024 Elsevier Inc. All rights reserved.
FAU - Lee, Siyeo
AU  - Lee S
AD  - Department of Pathology, Digestive Disease Research Institute, Wonkwang 
      University School of Medicine, Iksan, 54538, Republic of Korea; Department of 
      Biomedical Science, Graduate School Wonkwang University, Iksan, Jeonbuk, 54538, 
      Republic of Korea.
FAU - Memon, Azra
AU  - Memon A
AD  - Department of Pathology, Digestive Disease Research Institute, Wonkwang 
      University School of Medicine, Iksan, 54538, Republic of Korea.
FAU - Chae, Soo-Cheon
AU  - Chae SC
AD  - Department of Pathology, Digestive Disease Research Institute, Wonkwang 
      University School of Medicine, Iksan, 54538, Republic of Korea.
FAU - Shin, Donghun
AU  - Shin D
AD  - Department of Developmental Biology, McGowan Institute for Regenerative Medicine, 
      University of Pittsburgh, Pittsburgh, PA, 15260, USA.
FAU - Choi, Tae-Young
AU  - Choi TY
AD  - Department of Pathology, Digestive Disease Research Institute, Wonkwang 
      University School of Medicine, Iksan, 54538, Republic of Korea; Department of 
      Biomedical Science, Graduate School Wonkwang University, Iksan, Jeonbuk, 54538, 
      Republic of Korea. Electronic address: choity76@wku.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20240110
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Epithelial Cell Adhesion Molecule)
SB  - IM
MH  - Animals
MH  - Humans
MH  - *Zebrafish
MH  - Epithelial Cell Adhesion Molecule/genetics/metabolism
MH  - Liver/metabolism
MH  - Bile Ducts, Intrahepatic/metabolism
MH  - Hepatocytes/metabolism
MH  - Epithelial Cells/metabolism
MH  - *Cholangitis/pathology
MH  - Liver Regeneration
OTO - NOTNLM
OT  - Epcam
OT  - Intrahepatic duct
OT  - Liver progenitor cells (LPCs)
OT  - Liver regeneration
OT  - Primary biliary cholangitis (PBC)
OT  - Zebrafish
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/01/16 00:42
MHDA- 2024/02/02 06:43
CRDT- 2024/01/15 18:02
PHST- 2023/11/13 00:00 [received]
PHST- 2023/12/29 00:00 [revised]
PHST- 2024/01/09 00:00 [accepted]
PHST- 2024/02/02 06:43 [medline]
PHST- 2024/01/16 00:42 [pubmed]
PHST- 2024/01/15 18:02 [entrez]
AID - S0006-291X(24)00047-0 [pii]
AID - 10.1016/j.bbrc.2024.149512 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2024 Feb 12;696:149512. doi: 
      10.1016/j.bbrc.2024.149512. Epub 2024 Jan 10.