PMID- 38225955
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240117
IS  - 2056-3418 (Print)
IS  - 2056-3426 (Electronic)
IS  - 2056-3426 (Linking)
VI  - 11
DP  - 2024
TI  - Immediate implantation of ultrafine fiber slow-release system based on cell 
      electrospinning to induce osteogenesis of mesenchymal stem cells.
PG  - rbad113
LID - 10.1093/rb/rbad113 [doi]
LID - rbad113
AB  - This study presents the development and evaluation of a 
      poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB) ultrafine fiber slow-release 
      system for in vivo osteogenic induction of human umbilical cord mesenchymal stem 
      cells (HUCMSCs). Utilizing dual-nozzle and cell electrospinning techniques, the 
      system encapsulates L-ascorbic acid-2-phosphate magnesium (ASP), 
      beta-glycerophosphate sodium and dexamethasone (DEX) within the fibers, ensuring 
      sustained osteogenic differentiation. The scaffold's morphology, 
      characterization, hydrophilicity, mechanical properties and cellular behavior 
      were examined. Immediate subcutaneous implantation in rabbits was conducted to 
      observe its ectopic osteogenic induction effect. Successfully fabricated P34HB 
      ultrafine fiber slow-release system. Characterization confirmed the uniform 
      distribution of HUCMSCs and inducing components within the scaffold, with no 
      chemical reactions affecting the active components. In vitro tests showcased a 
      prolonged release of DEX and ASP, while biocompatibility assays highlighted the 
      scaffold's suitability for cellular growth. Alizarin Red, type I collagen, and 
      osteopontin (OPN) staining verified the scaffold's potent osteogenic induction 
      effect on HUCMSCs. Notably, immediate implantation into New Zealand White rabbits 
      led to significant new bone formation within 8 weeks. These findings underscore 
      the system's potential for immediate in vivo implantation without prior in vitro 
      induction, marking a promising advancement in bone tissue engineering.
CI  - (c) The Author(s) 2023. Published by Oxford University Press.
FAU - Lu, Tao
AU  - Lu T
AD  - Department of Orthopaedics, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang 550004, China.
AD  - Department of Orthopaedics, The First People's Hospital of Guiyang, Guiyang 
      550004, China.
FAU - Yang, Long
AU  - Yang L
AD  - Department of Orthopaedics, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang 550004, China.
AD  - Center for Tissue Engineering and Stem Cell Research, Guizhou Medical University, 
      Guiyang 550004, China.
FAU - Li, Zhuoyang
AU  - Li Z
AD  - Department of Otolaryngology Head and Neck Surgery, Xinqiao Hospital, Army 
      Medical University (Third Military Medical University), Chongqing 400037, China.
FAU - Liu, Yin
AU  - Liu Y
AD  - Center for Tissue Engineering and Stem Cell Research, Guizhou Medical University, 
      Guiyang 550004, China.
AD  - Department of Dental implant, Stomatological Hospital of Guiyang, Guiyang 550000, 
      China.
FAU - Xu, Shun'en
AU  - Xu S
AD  - Department of Orthopaedics, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang 550004, China.
AD  - Center for Tissue Engineering and Stem Cell Research, Guizhou Medical University, 
      Guiyang 550004, China.
FAU - Ye, Chuan
AU  - Ye C
AUID- ORCID: 0000-0001-5670-6289
AD  - Department of Orthopaedics, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang 550004, China.
AD  - Center for Tissue Engineering and Stem Cell Research, Guizhou Medical University, 
      Guiyang 550004, China.
LA  - eng
PT  - Journal Article
DEP - 20231225
PL  - England
TA  - Regen Biomater
JT  - Regenerative biomaterials
JID - 101652150
PMC - PMC10789307
OTO - NOTNLM
OT  - biomaterials
OT  - bone tissue engineering
OT  - cell electrospinning
OT  - immediate implantation
OT  - in vivo induction
EDAT- 2024/01/16 06:42
MHDA- 2024/01/16 06:43
PMCR- 2023/12/25
CRDT- 2024/01/16 03:37
PHST- 2023/08/23 00:00 [received]
PHST- 2023/11/21 00:00 [revised]
PHST- 2023/12/08 00:00 [accepted]
PHST- 2024/01/16 06:43 [medline]
PHST- 2024/01/16 06:42 [pubmed]
PHST- 2024/01/16 03:37 [entrez]
PHST- 2023/12/25 00:00 [pmc-release]
AID - rbad113 [pii]
AID - 10.1093/rb/rbad113 [doi]
PST - epublish
SO  - Regen Biomater. 2023 Dec 25;11:rbad113. doi: 10.1093/rb/rbad113. eCollection 
      2024.