PMID- 38229323
OWN - NLM
STAT- MEDLINE
DCOM- 20240118
LR  - 20240118
IS  - 1099-0461 (Electronic)
IS  - 1095-6670 (Linking)
VI  - 38
IP  - 1
DP  - 2024 Jan
TI  - miRNA-211-5p inhibition enhances the protective effect of hucMSC-derived exosome 
      in Abeta(1-40) -induced SH-SY5Y cells by increasing NEP expression.
PG  - e23624
LID - 10.1002/jbt.23624 [doi]
AB  - Exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs) could 
      alleviate Alzheimer's disease (AD) defects. Additionally, engineered exosomes are 
      more effective in treating diseases. In this study, we established an in vitro 
      model of AD by treating SH-SY5Y cells with Abeta(1-40) . We observed that incubation 
      with hucMSC-derived exosomes effectively protected SH-S5Y5 cells from Abeta(1-40) 
      -induced damage. Since NEP plays a central role in suppressing AD development, we 
      screened NEP-targeting miRNAs that are differentially expressed in control and AD 
      patients. We identified miR-211-5p as a potent repressor of NEP expression. 
      Exosomes purified from hucMSCs overexpressing miR-211-5p inhibitor exhibited 
      significantly greater efficiency than control exosomes in mitigating the injury 
      caused by Abeta(1-40) treatment. However, this enhanced protective effect was 
      nullified by the knockdown of NEP. These observations demonstrate that inhibition 
      of miR-211-5p has the potential to improve the efficacy of hucMSC-derived 
      exosomes in AD treatment by increasing NEP expression.
CI  - (c) 2023 The Authors. Journal of Biochemical and Molecular Toxicology published by 
      Wiley Periodicals LLC.
FAU - Chen, Huijing
AU  - Chen H
AD  - Central Laboratory, The Fifth Hospital of Xiamen, Xiamen, Fujian, China.
FAU - Huang, Zhongqin
AU  - Huang Z
AD  - Department of Nursing, The Fifth Hospital of Xiamen, Xiamen, Fujian, China.
FAU - Lei, Aidi
AU  - Lei A
AD  - Department of Neurology, The Fifth Hospital of Xiamen, Xiamen, Fujian, China.
FAU - Yu, Xiaowen
AU  - Yu X
AD  - Department of Laboratory Medicine, Xiamen Medical College, Xiamen, Fujian, China.
FAU - Shen, MeiLing
AU  - Shen M
AD  - Department of Laboratory Medicine, Xiamen Medical College, Xiamen, Fujian, China.
FAU - Wu, Dan
AU  - Wu D
AUID- ORCID: 0009-0007-9693-1588
AD  - Central Laboratory, The Fifth Hospital of Xiamen, Xiamen, Fujian, China.
AD  - Xiamen Key Laboratory of Precision Diagnosis and Treatment of Chronic Kidney 
      Disease, Xiamen, Fujian, China.
LA  - eng
GR  - 2021D013/Science and Technology Plan Project of Fujian Province/
GR  - 3502Z20214ZD1266/Xiamen Medical and Health Guidance Project/
PT  - Journal Article
PL  - United States
TA  - J Biochem Mol Toxicol
JT  - Journal of biochemical and molecular toxicology
JID - 9717231
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN211 microRNA, human)
SB  - IM
MH  - Humans
MH  - *Exosomes/metabolism
MH  - *Neuroblastoma/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *Alzheimer Disease/therapy/metabolism
MH  - Umbilical Cord/metabolism
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - human umbilical cord mesenchymal stem cell-exosomes
OT  - microRNA-211-5p inhibitor
OT  - neprilysin
EDAT- 2024/01/17 06:42
MHDA- 2024/01/18 06:42
CRDT- 2024/01/17 01:03
PHST- 2023/10/24 00:00 [revised]
PHST- 2023/04/17 00:00 [received]
PHST- 2023/12/14 00:00 [accepted]
PHST- 2024/01/18 06:42 [medline]
PHST- 2024/01/17 06:42 [pubmed]
PHST- 2024/01/17 01:03 [entrez]
AID - 10.1002/jbt.23624 [doi]
PST - ppublish
SO  - J Biochem Mol Toxicol. 2024 Jan;38(1):e23624. doi: 10.1002/jbt.23624.