PMID- 38233907
OWN - NLM
STAT- MEDLINE
DCOM- 20240119
LR  - 20240201
IS  - 1741-7007 (Electronic)
IS  - 1741-7007 (Linking)
VI  - 22
IP  - 1
DP  - 2024 Jan 17
TI  - The accumulation of modular serine protease mediated by a novel circRNA sponging 
      miRNA increases Aedes aegypti immunity to fungus.
PG  - 7
LID - 10.1186/s12915-024-01811-6 [doi]
LID - 7
AB  - BACKGROUND: Mosquitoes transmit many infectious diseases that affect human 
      health. The fungus Beauveria bassiana is a biological pesticide that is 
      pathogenic to mosquitoes but harmless to the environment. RESULTS: We found a 
      microRNA (miRNA) that can modulate the antifungal immunity of Aedes aegypti by 
      inhibiting its cognate serine protease. Fungal infection can induce the 
      expression of modular serine protease (ModSP), and ModSP knockdown mosquitoes 
      were more sensitive to B. bassiana infection. The novel miRNA-novel-53 is linked 
      to antifungal immune response and was greatly diminished in infected mosquitoes. 
      The miRNA-novel-53 could bind to the coding sequences of ModSP and impede its 
      expression. Double fluorescence in situ hybridization (FISH) showed that this 
      inhibition occurred in the cytoplasm. The amount of miRNA-novel-53 increased 
      after miRNA agomir injection. This resulted in a significant decrease in ModSP 
      transcript and a significant increase in mortality after fungal infection. An 
      opposite effect was produced after antagomir injection. The miRNA-novel-53 was 
      also knocked out using CRISPR-Cas9, which increased mosquito resistance to the 
      fungus B. bassiana. Moreover, mosquito novel-circ-930 can affect ModSP mRNA by 
      interacting with miRNA-novel-53 during transfection with siRNA or overexpression 
      plasmid. CONCLUSIONS: Novel-circ-930 affects the expression level of ModSP by a 
      novel-circ-930/miRNA-novel-53/ModSP mechanism to modulate antifungal immunity, 
      revealing new information on innate immunity in insects.
CI  - (c) 2024. The Author(s).
FAU - Lu, Tengfei
AU  - Lu T
AD  - State Key Laboratory of Integrated Management of Pest Insects and Rodents, 
      Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
AD  - CAS Center for Excellence in Biotic Interactions, University of Chinese Academy 
      of Sciences, Beijing, 100049, China.
FAU - Ji, Yannan
AU  - Ji Y
AD  - State Key Laboratory of Integrated Management of Pest Insects and Rodents, 
      Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
AD  - CAS Center for Excellence in Biotic Interactions, University of Chinese Academy 
      of Sciences, Beijing, 100049, China.
FAU - Chang, Mengmeng
AU  - Chang M
AD  - State Key Laboratory of Integrated Management of Pest Insects and Rodents, 
      Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
AD  - CAS Center for Excellence in Biotic Interactions, University of Chinese Academy 
      of Sciences, Beijing, 100049, China.
FAU - Zhang, Xiaoming
AU  - Zhang X
AD  - State Key Laboratory of Integrated Management of Pest Insects and Rodents, 
      Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
AD  - CAS Center for Excellence in Biotic Interactions, University of Chinese Academy 
      of Sciences, Beijing, 100049, China.
FAU - Wang, Yanhong
AU  - Wang Y
AD  - State Key Laboratory of Integrated Management of Pest Insects and Rodents, 
      Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. 
      wangyanhong@ioz.ac.cn.
AD  - CAS Center for Excellence in Biotic Interactions, University of Chinese Academy 
      of Sciences, Beijing, 100049, China. wangyanhong@ioz.ac.cn.
FAU - Zou, Zhen
AU  - Zou Z
AUID- ORCID: 0000-0003-3550-7656
AD  - State Key Laboratory of Integrated Management of Pest Insects and Rodents, 
      Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. 
      zouzhen@ioz.ac.cn.
AD  - CAS Center for Excellence in Biotic Interactions, University of Chinese Academy 
      of Sciences, Beijing, 100049, China. zouzhen@ioz.ac.cn.
LA  - eng
GR  - ZDBS-LY-SM027/Innovative program of the CAS/
GR  - 82372288/National Natural Science Foundation of China/
GR  - 32370522/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20240117
PL  - England
TA  - BMC Biol
JT  - BMC biology
JID - 101190720
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - EC 3.4.- (Serine Proteases)
RN  - 0 (Antifungal Agents)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Animals
MH  - Humans
MH  - *Aedes/genetics/microbiology
MH  - *MicroRNAs/genetics
MH  - RNA, Circular
MH  - Serine Proteases/genetics
MH  - Antifungal Agents
MH  - In Situ Hybridization, Fluorescence
MH  - Fungi/genetics
MH  - *Mycoses
MH  - Serine Endopeptidases
PMC - PMC10795361
OTO - NOTNLM
OT  - Aedes aegypti
OT  - Antifungal immunity
OT  - Beauveria bassiana
OT  - ModSP
OT  - miRNA
COIS- The authors declare that they have no competing interests.
EDAT- 2024/01/18 00:42
MHDA- 2024/01/19 06:42
PMCR- 2024/01/17
CRDT- 2024/01/17 23:41
PHST- 2023/02/07 00:00 [received]
PHST- 2024/01/02 00:00 [accepted]
PHST- 2024/01/19 06:42 [medline]
PHST- 2024/01/18 00:42 [pubmed]
PHST- 2024/01/17 23:41 [entrez]
PHST- 2024/01/17 00:00 [pmc-release]
AID - 10.1186/s12915-024-01811-6 [pii]
AID - 1811 [pii]
AID - 10.1186/s12915-024-01811-6 [doi]
PST - epublish
SO  - BMC Biol. 2024 Jan 17;22(1):7. doi: 10.1186/s12915-024-01811-6.