PMID- 38234967
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240119
IS  - 1611-2156 (Print)
IS  - 1611-2156 (Electronic)
IS  - 1611-2156 (Linking)
VI  - 22
DP  - 2023
TI  - Alpha-ketoglutarate partially alleviates effects of high-fat high-fructose diet 
      in mouse muscle.
PG  - 1264-1277
LID - 10.17179/excli2023-6608 [doi]
AB  - Consumption of high-calorie diets leads to excessive accumulation of storage 
      lipids in adipose tissue. Metabolic changes occur not only in adipose tissue but 
      in other tissues, too, such as liver, heart, muscle, and brain. This study aimed 
      to explore the effects of high-fat high-fructose diet (HFFD) alone and in the 
      combination with alpha-ketoglutarate (AKG), a well-known cellular metabolite, on 
      energy metabolism in the skeletal muscle of C57BL/6J mice. Five-month-old male 
      mice were divided into four groups - the control one fed a standard diet (10 % 
      kcal fat), HFFD group fed a high-fat high-fructose diet (45 % kcal fat, 15 % kcal 
      fructose), AKG group fed a standard diet with 1 % sodium AKG in drinking water, 
      and HFFD + AKG group fed HFFD and water with 1 % sodium AKG. The dietary regimens 
      lasted 8 weeks. Mice fed HFFD had higher levels of storage triacylglycerides, 
      lower levels of glycogen, and total water-soluble protein, and higher activities 
      of key glycolytic enzymes, namely hexokinase, phosphofructokinase, and pyruvate 
      kinase, as compared with the control group. The results suggest that muscles of 
      HFFD mice may suffer from lipotoxicity. In HFFD + AKG mice, levels of the 
      metabolites and activities of glycolytic enzymes did not differ from the 
      respective values in the control group, except for the activity of pyruvate 
      kinase, which was significantly lower in HFFD + AKG group compared with the 
      control. Thus, metabolic changes in mouse skeletal muscles, caused by HFFD, were 
      alleviated by AKG, indicating a protective role of AKG regarding lipotoxicity.
CI  - Copyright (c) 2023 Vatashchuk et al.
FAU - Vatashchuk, Myroslava V
AU  - Vatashchuk MV
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
FAU - Bayliak, Maria M
AU  - Bayliak MM
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
FAU - Hurza, Viktoriia V
AU  - Hurza VV
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
FAU - Demianchuk, Oleh I
AU  - Demianchuk OI
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
FAU - Gospodaryov, Dmytro V
AU  - Gospodaryov DV
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
FAU - Lushchak, Volodymyr I
AU  - Lushchak VI
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
AD  - Research and Development University, 13a Shota Rustaveli Str., Ivano-Frankivsk, 
      76018, Ukraine.
LA  - eng
PT  - Journal Article
DEP - 20231205
PL  - Germany
TA  - EXCLI J
JT  - EXCLI journal
JID - 101299402
PMC - PMC10792174
OTO - NOTNLM
OT  - energy metabolism
OT  - glycolysis
OT  - high-fat high-fructose diet
OT  - lipotoxicity
OT  - mouse
COIS- The authors declare that they have no conflict of interest.
EDAT- 2024/01/18 06:42
MHDA- 2024/01/18 06:43
PMCR- 2023/12/05
CRDT- 2024/01/18 04:16
PHST- 2023/09/16 00:00 [received]
PHST- 2023/11/14 00:00 [accepted]
PHST- 2024/01/18 06:43 [medline]
PHST- 2024/01/18 06:42 [pubmed]
PHST- 2024/01/18 04:16 [entrez]
PHST- 2023/12/05 00:00 [pmc-release]
AID - 2023-6608 [pii]
AID - Doc1264 [pii]
AID - 10.17179/excli2023-6608 [doi]
PST - epublish
SO  - EXCLI J. 2023 Dec 5;22:1264-1277. doi: 10.17179/excli2023-6608. eCollection 2023.