PMID- 38238469
OWN - NLM
STAT- MEDLINE
DCOM- 20240208
LR  - 20240329
IS  - 2058-5276 (Electronic)
IS  - 2058-5276 (Linking)
VI  - 9
IP  - 2
DP  - 2024 Feb
TI  - Yersinia entomophaga Tc toxin is released by T10SS-dependent lysis of specialized 
      cell subpopulations.
PG  - 390-404
LID - 10.1038/s41564-023-01571-z [doi]
AB  - Disease-causing bacteria secrete numerous toxins to invade and subjugate their 
      hosts. Unlike many smaller toxins, the secretion machinery of most large toxins 
      remains enigmatic. By combining genomic editing, proteomic profiling and 
      cryo-electron tomography of the insect pathogen Yersinia entomophaga, we 
      demonstrate that a specialized subset of these cells produces a complex toxin 
      cocktail, including the nearly ribosome-sized Tc toxin YenTc, which is 
      subsequently exported by controlled cell lysis using a transcriptionally coupled, 
      pH-dependent type 10 secretion system (T10SS). Our results dissect the Tc toxin 
      export process by a T10SS, identifying that T10SSs operate via a previously 
      unknown lytic mode of action and establishing them as crucial players in the 
      size-insensitive release of cytoplasmically folded toxins. With T10SSs directly 
      embedded in Tc toxin operons of major pathogens, we anticipate that our findings 
      may model an important aspect of pathogenesis in bacteria with substantial impact 
      on agriculture and healthcare.
CI  - (c) 2024. The Author(s).
FAU - Sitsel, Oleg
AU  - Sitsel O
AUID- ORCID: 0000-0002-4496-7489
AD  - Department of Structural Biochemistry, Max Planck Institute of Molecular 
      Physiology, Dortmund, Germany.
FAU - Wang, Zhexin
AU  - Wang Z
AUID- ORCID: 0000-0002-4256-1143
AD  - Department of Structural Biochemistry, Max Planck Institute of Molecular 
      Physiology, Dortmund, Germany.
FAU - Janning, Petra
AU  - Janning P
AD  - Department of Chemical Biology, Max Planck Institute of Molecular Physiology, 
      Dortmund, Germany.
FAU - Kroczek, Lara
AU  - Kroczek L
AD  - Department of Structural Biochemistry, Max Planck Institute of Molecular 
      Physiology, Dortmund, Germany.
FAU - Wagner, Thorsten
AU  - Wagner T
AD  - Department of Structural Biochemistry, Max Planck Institute of Molecular 
      Physiology, Dortmund, Germany.
FAU - Raunser, Stefan
AU  - Raunser S
AUID- ORCID: 0000-0001-9373-3016
AD  - Department of Structural Biochemistry, Max Planck Institute of Molecular 
      Physiology, Dortmund, Germany. stefan.raunser@mpi-dortmund.mpg.de.
LA  - eng
PT  - Journal Article
DEP - 20240118
PL  - England
TA  - Nat Microbiol
JT  - Nature microbiology
JID - 101674869
RN  - Yersinia entomophaga
SB  - IM
MH  - *Proteomics
MH  - *Yersinia/genetics/metabolism
PMC - PMC10847048
COIS- O.S. and S.R. are inventors on a filed patent application (PCT/EP2023/074766) for 
      producing insecticidal toxins using the described bacterial strains. The 
      remaining authors declare no competing interests.
EDAT- 2024/01/19 00:42
MHDA- 2024/02/08 06:42
PMCR- 2024/01/18
CRDT- 2024/01/18 23:23
PHST- 2023/03/28 00:00 [received]
PHST- 2023/11/29 00:00 [accepted]
PHST- 2024/02/08 06:42 [medline]
PHST- 2024/01/19 00:42 [pubmed]
PHST- 2024/01/18 23:23 [entrez]
PHST- 2024/01/18 00:00 [pmc-release]
AID - 10.1038/s41564-023-01571-z [pii]
AID - 1571 [pii]
AID - 10.1038/s41564-023-01571-z [doi]
PST - ppublish
SO  - Nat Microbiol. 2024 Feb;9(2):390-404. doi: 10.1038/s41564-023-01571-z. Epub 2024 
      Jan 18.