PMID- 38239224
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240121
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 14
DP  - 2023
TI  - Phaseolus vulgaris mutants reveal variation in the nuclear genome.
PG  - 1308830
LID - 10.3389/fpls.2023.1308830 [doi]
LID - 1308830
AB  - Phaseolus vulgaris L. (common bean) is an essential source of proteins in the 
      human diet worldwide. Bean breeding programs to increase genetic diversity based 
      on induced mutagenesis have a long tradition in Bulgaria. Common bean varieties 
      with high productivity, wide environmental adaptability, good nutritional 
      properties, and improved disease resistance have been successfully developed. In 
      this study, we aimed to investigate selected nuclear genome features, such as the 
      genome size, the number and chromosomal distribution of 5S and 35S rDNA loci by 
      using the fluorescence in situ hybridization (FISH), as well as the level of DNA 
      damage in some local Bulgarian accessions and mutants of P. vulgaris. Flow 
      cytometry analyses revealed no significant differences in genome size between 
      analyzed lines except for one of the analyzed mutants, M19. The value of genome 
      size 2C DNA is about 1.37 pg2C (-1) for all lines, whereas it is 1.42 pg2C(-1) 
      for M19. The chromosome number remains the same (2n=22) for all analyzed lines. 
      Results of FISH analyses showed that the number of 5S rDNA was stable among 
      accessions and mutant lines (four loci), while the number of 35S rDNA loci was 
      shown as highly polymorphic, varying between ten and sixteen, and displaying 
      differences in the size and location of 35S rDNA loci between analyzed genotypes. 
      The cell cycle profile was different for the analyzed genotypes. The results 
      revealed that wide variation in genome organization and size as well as DNA 
      damage characterizes the analyzed genetic resources of the common bean.
CI  - Copyright (c) 2024 Tomlekova, Idziak-Helmcke, Franke, Rojek-Jelonek and 
      Kwasniewska.
FAU - Tomlekova, Nasya
AU  - Tomlekova N
AD  - Laboratory of Molecular Biology, Department of Breeding, Marisa Vegetable Crops 
      Research Institute, Plovdiv, Agricultural Academy, Sofia, Bulgaria.
FAU - Idziak-Helmcke, Dominika
AU  - Idziak-Helmcke D
AD  - Plant Cytogenetics and Molecular Biology Group, Faculty of Natural Sciences, 
      University of Silesia in Katowice, Katowice, Poland.
FAU - Franke, Paula
AU  - Franke P
AD  - Plant Cytogenetics and Molecular Biology Group, Faculty of Natural Sciences, 
      University of Silesia in Katowice, Katowice, Poland.
FAU - Rojek-Jelonek, Magdalena
AU  - Rojek-Jelonek M
AD  - Plant Cytogenetics and Molecular Biology Group, Faculty of Natural Sciences, 
      University of Silesia in Katowice, Katowice, Poland.
FAU - Kwasniewska, Jolanta
AU  - Kwasniewska J
AD  - Plant Cytogenetics and Molecular Biology Group, Faculty of Natural Sciences, 
      University of Silesia in Katowice, Katowice, Poland.
LA  - eng
PT  - Journal Article
DEP - 20240104
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC10794375
OTO - NOTNLM
OT  - chemical mutagenesis
OT  - DNA damage
OT  - FISH
OT  - cell cycle
OT  - common bean
OT  - genome size
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2024/01/19 06:42
MHDA- 2024/01/19 06:43
PMCR- 2023/01/01
CRDT- 2024/01/19 03:36
PHST- 2023/10/07 00:00 [received]
PHST- 2023/12/08 00:00 [accepted]
PHST- 2024/01/19 06:43 [medline]
PHST- 2024/01/19 06:42 [pubmed]
PHST- 2024/01/19 03:36 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2023.1308830 [doi]
PST - epublish
SO  - Front Plant Sci. 2024 Jan 4;14:1308830. doi: 10.3389/fpls.2023.1308830. 
      eCollection 2023.