PMID- 38240346
OWN - NLM
STAT- MEDLINE
DCOM- 20240315
LR  - 20240316
IS  - 1939-1676 (Electronic)
IS  - 0891-6640 (Print)
IS  - 0891-6640 (Linking)
VI  - 38
IP  - 2
DP  - 2024 Mar-Apr
TI  - Serum symmetric dimethylarginine in older dogs: Reference interval and comparison 
      of a gold standard method with the ELISA.
PG  - 960-970
LID - 10.1111/jvim.16981 [doi]
AB  - BACKGROUND: Serum symmetric dimethylarginine (SDMA) is used to screen for renal 
      dysfunction in dogs. The gold standard technique for measuring SDMA, liquid 
      chromatography-tandem mass spectrometry (LC-MS/MS) is not widely available. 
      Age-specific reference intervals for SDMA in older dogs are lacking. OBJECTIVES: 
      Prospective study in older dogs to validate a commercially available LC-MS/MS 
      method for SDMA, compare SDMA concentrations with concentrations measured using 
      ELISA and obtain a reference interval (RI) for older dogs using both methods. 
      ANIMALS: Client-owned older dogs undergoing health screening. METHODS: The 
      LC-MS/MS method was analytically validated (limit of detection, precision, and 
      linearity). Serum was sent cooled overnight for ELISA or was frozen at -80 degrees C 
      until batch analysis using LC-MS/MS. Results of LC-MS/MS and ELISA were compared 
      and RIs for older dogs were calculated according to international guidelines. 
      RESULTS: The LC-MS/MS method showed good linearity (r(2)  = .99) and precision 
      (coefficient of variation <10%), with a laboratory RI between 8.0 and 14.0 mug/dL. 
      Paired measurements were available from 118 different dogs. Median SDMA 
      concentration were 9.4 (range, 5.0-21.2) using LC-MS/MS and 12.0 (range, 
      5.0-22.0) mug/dL using ELISA. Both methods significantly differed with a mean 
      difference of 2.2 mug/dL. The RI for older dogs for LC-MS/MS was 4.4-15.0 mug/dL, 
      and for ELISA was 6.4-17.4 mug/dL. CONCLUSIONS AND CLINICAL IMPORTANCE: The ELISA 
      provided significantly higher SDMA concentrations compared to the validated 
      LC-MS/MS method, indicating the need for device- or assay-specific RI. The 
      obtained age-specific RI for SDMA is considerably higher in older dogs compared 
      to the general laboratory RI.
CI  - (c) 2024 The Authors. Journal of Veterinary Internal Medicine published by Wiley 
      Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
FAU - Marynissen, Sofie
AU  - Marynissen S
AUID- ORCID: 0000-0002-1884-9366
AD  - Small Animal Department, Faculty of Veterinary Medicine, Ghent University, 
      Merelbeke, Belgium.
FAU - Junius, Greet
AU  - Junius G
AD  - Sonic Healthcare Benelux, Antwerpen, Belgium.
FAU - Van den Steen, Evi
AU  - Van den Steen E
AD  - Sonic Healthcare Benelux, Antwerpen, Belgium.
FAU - Patteet, Lisbeth
AU  - Patteet L
AD  - Sonic Healthcare Benelux, Antwerpen, Belgium.
FAU - Duchateau, Luc
AU  - Duchateau L
AD  - Biometrics Research Group, Department of Veterinary and Biosciences, Faculty of 
      Veterinary Medicine, Ghent University, Merelbeke, Belgium.
FAU - Croubels, Siska
AU  - Croubels S
AD  - Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of 
      Veterinary Medicine, Ghent University, Merelbeke, Belgium.
FAU - Daminet, Sylvie
AU  - Daminet S
AUID- ORCID: 0000-0001-6733-7925
AD  - Small Animal Department, Faculty of Veterinary Medicine, Ghent University, 
      Merelbeke, Belgium.
FAU - Paepe, Dominique
AU  - Paepe D
AUID- ORCID: 0000-0003-2919-1712
AD  - Small Animal Department, Faculty of Veterinary Medicine, Ghent University, 
      Merelbeke, Belgium.
LA  - eng
PT  - Journal Article
DEP - 20240119
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 49787G1ULV (symmetric dimethylarginine)
RN  - 94ZLA3W45F (Arginine)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Humans
MH  - Dogs
MH  - Animals
MH  - Chromatography, Liquid/veterinary
MH  - Prospective Studies
MH  - Tandem Mass Spectrometry/veterinary
MH  - *Renal Insufficiency, Chronic/veterinary
MH  - Arginine/*analogs & derivatives
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Biomarkers
MH  - *Dog Diseases/diagnosis
PMC - PMC10937498
OTO - NOTNLM
OT  - ELISA
OT  - SDMA
OT  - asymmetric dimethylarginine
OT  - canine
COIS- Greet Junius, Evi Van den Steen and Lisbeth Patteet are professionally affiliated 
      with the commercial laboratory (SONIC Healthcare Benelux) that validated and 
      performed the LC-MS/MS analysis. They were not involved in study design, results 
      processing, and statistical analyses of the acquired data. No other authors 
      declare a conflict of interest.
EDAT- 2024/01/19 12:44
MHDA- 2024/03/15 06:44
PMCR- 2024/01/19
CRDT- 2024/01/19 08:19
PHST- 2023/04/27 00:00 [received]
PHST- 2023/12/15 00:00 [accepted]
PHST- 2024/03/15 06:44 [medline]
PHST- 2024/01/19 12:44 [pubmed]
PHST- 2024/01/19 08:19 [entrez]
PHST- 2024/01/19 00:00 [pmc-release]
AID - JVIM16981 [pii]
AID - 10.1111/jvim.16981 [doi]
PST - ppublish
SO  - J Vet Intern Med. 2024 Mar-Apr;38(2):960-970. doi: 10.1111/jvim.16981. Epub 2024 
      Jan 19.