PMID- 38247922
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240128
IS  - 2306-5354 (Print)
IS  - 2306-5354 (Electronic)
IS  - 2306-5354 (Linking)
VI  - 11
IP  - 1
DP  - 2024 Jan 1
TI  - Nuclear Factor-kappaB Decoy Oligodeoxynucleotide Attenuates Cartilage Resorption In 
      Vitro.
LID - 10.3390/bioengineering11010046 [doi]
LID - 46
AB  - BACKGROUND: Cartilage harvest and transplantation is a common surgery using 
      costal, auricular, and septal cartilage for craniofacial reconstruction. However, 
      absorption and warping of the cartilage grafts can occur due to inflammatory 
      factors associated with wound healing. Transcription factor nuclear factor-kappaB 
      (NF-kappaB) is activated by the various stimulation such as interleukin-1 (IL-1), and 
      plays a central role in the transactivation of this inflammatory cytokine gene. 
      Inhibition of NF-kappaB may have anti-inflammatory effects. The aim of this study was 
      to explore the potential of an NF-kappaB decoy oligodeoxynucleotide (Decoy) as a 
      chondroprotective agent. MATERIALS AND METHODS: Safe and efficacious 
      concentrations of Decoy were assessed using rabbit nasal septal chondrocytes 
      (rNSChs) and assays for cytotoxicity, proteoglycan (PG) synthesis, and PG 
      turnover were carried out. The efficacious concentration of Decoy determined from 
      the rNSChs was then applied to human nasal septal cartilage (hNSC) in vitro and 
      analyzed for PG turnover, the levels of inflammatory markers, and catabolic 
      enzymes in explant-conditioned culture medium. RESULTS: Over the range of Decoy 
      conditions and concentrations, no inhibition of PG synthesis or cytotoxicity was 
      observed. Decoy at 10 muM effectively inhibited PG degradation in the hNSC 
      explant, prolonging PG half-life by 63% and decreasing matrix metalloprotease 3 
      (MMP-3) by 70.7% (p = 0.027). CONCLUSIONS: Decoy may be considered a novel 
      chondroprotective therapeutic agent in cartilage transplantation due to its 
      ability to inhibit cartilage degradation due to inflammation cytokines.
FAU - Nemoto, Hitoshi
AU  - Nemoto H
AD  - Department of Otolaryngology-Head and Neck Surgery, School of Medicine, 
      University of California, La Jolla, San Diego, CA 92093, USA.
AD  - Department of Plastic Surgery, School of Medicine, Tokai University, Isehara 
      259-1193, Kanagawa, Japan.
FAU - Sakai, Daisuke
AU  - Sakai D
AUID- ORCID: 0000-0003-4189-9270
AD  - Department of Orthopaedic Surgery, School of Medicine, University of California, 
      La Jolla, San Diego, CA 92093, USA.
AD  - Department of Orthopaedic Surgery, School of Medicine, Tokai University, Isehara 
      259-1193, Kanagawa, Japan.
FAU - Watson, Deborah
AU  - Watson D
AD  - Department of Otolaryngology-Head and Neck Surgery, School of Medicine, 
      University of California, La Jolla, San Diego, CA 92093, USA.
FAU - Masuda, Koichi
AU  - Masuda K
AUID- ORCID: 0000-0002-5361-4415
AD  - Department of Orthopaedic Surgery, School of Medicine, University of California, 
      La Jolla, San Diego, CA 92093, USA.
LA  - eng
PT  - Journal Article
DEP - 20240101
PL  - Switzerland
TA  - Bioengineering (Basel)
JT  - Bioengineering (Basel, Switzerland)
JID - 101676056
PMC - PMC10813736
OTO - NOTNLM
OT  - cartilage
OT  - decoy oligodeoxynucleotide
OT  - nuclear factor-kappab
OT  - resorption
COIS- K.M. has received research non-related grants from AnGes inc. K.M. and D.S. have 
      been involved as consultants in AnGes Inc. for different applications. H.N. and 
      D.W. have no conflicts of interest.
EDAT- 2024/01/22 06:43
MHDA- 2024/01/22 06:44
PMCR- 2024/01/01
CRDT- 2024/01/22 04:34
PHST- 2023/10/25 00:00 [received]
PHST- 2023/12/20 00:00 [revised]
PHST- 2023/12/22 00:00 [accepted]
PHST- 2024/01/22 06:44 [medline]
PHST- 2024/01/22 06:43 [pubmed]
PHST- 2024/01/22 04:34 [entrez]
PHST- 2024/01/01 00:00 [pmc-release]
AID - bioengineering11010046 [pii]
AID - bioengineering-11-00046 [pii]
AID - 10.3390/bioengineering11010046 [doi]
PST - epublish
SO  - Bioengineering (Basel). 2024 Jan 1;11(1):46. doi: 10.3390/bioengineering11010046.