PMID- 38253544
OWN - NLM
STAT- MEDLINE
DCOM- 20240220
LR  - 20240220
IS  - 2576-6422 (Electronic)
IS  - 2576-6422 (Linking)
VI  - 7
IP  - 2
DP  - 2024 Feb 19
TI  - Host-Guest Mechanism via Induced Fit Fullerene Complexation in Porphin Receptor 
      to Probe Salivary Alpha-Amylase in Dental Caries for Clinical Applications.
PG  - 1250-1259
LID - 10.1021/acsabm.3c01189 [doi]
AB  - Salivary alpha-amylase is the most abundant protein of human saliva that potentially 
      binds to streptococcus and other bacteria via specific surface-exposed 
      alpha-amylase-binding proteins and plays a significant role in caries development. 
      The detection of alpha-amylase in saliva can be used as a bioindicator of caries 
      development. Herein, a facile strategy has been applied, tailoring the 
      photochemical properties of 5,10,15,20-tetrakis(4-hydroxyphenyl)-21H,23H-porphine 
      (TPPOH) and the fullerene C60 complex. The fluorescence emission of TPPOH is 
      quenched by starch-coated fullerene C60 via charge-transfer effects, as 
      determined by UV absorption and fluorescence spectroscopic studies. The 
      starch-coated C60 has been thoroughly characterized via Fourier transform 
      infrared (FTIR) spectroscopy, X-ray diffraction (XRD), field emission scanning 
      electron microscopy (FE-SEM), optical microscopy, thermal gravimetric analysis 
      (TGA), static water contact angle measurements, and zeta potential measurements. 
      The analytical response of the assay showed a linear fluorescent response in 
      alpha-amylase concentrations ranging from 0.001-0.1 Units/mL, with an LOD of 0.001 
      Units/mL. The applicability of the method was tested using artificial saliva with 
      quantitative recoveries in the range 95-100%. The practicability of the procedure 
      was verified by inspecting saliva samples of real clinical samples covering all 
      age groups. We believe that the proposed method can serve as an alternative 
      analytical method for caries detection and risk assessment that would also 
      minimize the cost of professional preventive measures and treatments.
FAU - Asghar, Hira
AU  - Asghar H
AD  - Institute of Molecular Biology and Biotechnology, The University of Lahore, 1-Km 
      Defence Road, Near Bhuptian Chowk, Lahore 54000, Pakistan.
AD  - Interdisciplinary Research Center in Biomedical Materials (IRCBM), COMSATS 
      University, Islamabad, Lahore Campus, Lahore 54000, Pakistan.
AD  - Azra Naheed Dental College, Superior University, Raiwind Road, Lahore 54000, 
      Pakistan.
FAU - Bilal, Sehrish
AU  - Bilal S
AD  - Department of Biochemistry, Gulab Devi Educational Complex, Ferozepur Road, 
      Lahore 54600, Pakistan.
FAU - Nawaz, Mian Hasnain
AU  - Nawaz MH
AUID- ORCID: 0000-0002-6648-0778
AD  - Interdisciplinary Research Center in Biomedical Materials (IRCBM), COMSATS 
      University, Islamabad, Lahore Campus, Lahore 54000, Pakistan.
FAU - Rasool, Ghulam
AU  - Rasool G
AD  - Institute of Molecular Biology and Biotechnology, The University of Lahore, 1-Km 
      Defence Road, Near Bhuptian Chowk, Lahore 54000, Pakistan.
FAU - Hayat, Akhtar
AU  - Hayat A
AUID- ORCID: 0000-0002-3123-581X
AD  - Interdisciplinary Research Center in Biomedical Materials (IRCBM), COMSATS 
      University, Islamabad, Lahore Campus, Lahore 54000, Pakistan.
LA  - eng
PT  - Journal Article
DEP - 20240122
PL  - United States
TA  - ACS Appl Bio Mater
JT  - ACS applied bio materials
JID - 101729147
RN  - EC 3.2.1.1 (Salivary alpha-Amylases)
RN  - 604BGD9J5B (porphine)
RN  - NP9U26B839 (fullerene C60)
RN  - 0 (Fullerenes)
RN  - 9005-25-8 (Starch)
RN  - 0 (Porphyrins)
SB  - IM
MH  - Humans
MH  - *Salivary alpha-Amylases
MH  - *Fullerenes/chemistry
MH  - *Dental Caries
MH  - Starch/metabolism
MH  - Microscopy, Electron, Scanning
MH  - *Porphyrins
OTO - NOTNLM
OT  - alpha-amylase
OT  - clinical patients
OT  - dental caries
OT  - fluorescence quenching
OT  - fullerene-porphyrin complex
OT  - human saliva
OT  - starch-coated C60
EDAT- 2024/01/23 00:42
MHDA- 2024/02/20 11:50
CRDT- 2024/01/22 23:33
PHST- 2024/02/20 11:50 [medline]
PHST- 2024/01/23 00:42 [pubmed]
PHST- 2024/01/22 23:33 [entrez]
AID - 10.1021/acsabm.3c01189 [doi]
PST - ppublish
SO  - ACS Appl Bio Mater. 2024 Feb 19;7(2):1250-1259. doi: 10.1021/acsabm.3c01189. Epub 
      2024 Jan 22.