PMID- 38254449 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240128 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 14 IP - 2 DP - 2024 Jan 16 TI - Evaluation of Truck Cab Decontamination Procedures following Inoculation with Porcine Epidemic Diarrhea Virus and Porcine Reproductive and Respiratory Syndrome Virus. LID - 10.3390/ani14020280 [doi] LID - 280 AB - This experiment aimed to evaluate commercially available disinfectants and their application methods against porcine epidemic diarrhea virus (PEDV) and porcine reproductive and respiratory syndrome virus (PRRSV) on truck cab surfaces. Plastic, fabric, and rubber surfaces inoculated with PEDV or PRRSV were placed in a full-scale truck cab and then treated with one of eight randomly assigned disinfectant treatments. After application, surfaces were environmentally sampled with cotton gauze and tested for PEDV and PRRSV using qPCR duplex analysis. There was a disinfectant x surface interaction (p < 0.0001), indicating a detectable amount of PEDV or PRRSV RNA was impacted by disinfectant treatment and surface material. For rubber surfaces, 10% bleach application had lower detectable amounts of RNA compared to all other treatments (p < 0.05) except Intervention via misting fumigation, which was intermediate. In both fabric and plastic surfaces, there was no evidence (p > 0.05) of a difference in detectable RNA between disinfectant treatments. For disinfectant treatments, fabric surfaces with no chemical treatment had less detectable viral RNA compared to the corresponding plastic and rubber (p < 0.05). Intervention applied via pump sprayer to fabric surfaces had less detectable viral RNA than plastic (p < 0.05). Furthermore, 10% bleach applied via pump sprayer to fabric and rubber surfaces had less detectable viral RNA than plastic (p < 0.05). Also, a 10 h downtime, with no chemical application or gaseous fumigation for 10 h, applied to fabric surfaces had less detectable viral RNA than other surfaces (p < 0.05). Sixteen treatments were evaluated via swine bioassay, but all samples failed to produce infectivity. In summary, commercially available disinfectants successfully reduced detectable viral RNA on surfaces but did not eliminate viral genetic material, highlighting the importance of bioexclusion of pathogens of interest. FAU - Houston, Grace E AU - Houston GE AUID- ORCID: 0000-0001-8113-1616 AD - Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-0201, USA. FAU - Jones, Cassandra K AU - Jones CK AD - Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA. FAU - Evans, Caitlin AU - Evans C AD - Department of Grain Science and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA. FAU - Otott, Haley K AU - Otott HK AD - Department of Grain Science and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA. FAU - Stark, Charles R AU - Stark CR AD - Department of Grain Science and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA. FAU - Bai, Jianfa AU - Bai J AD - Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-0201, USA. FAU - Poulsen Porter, Elizabeth G AU - Poulsen Porter EG AD - Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-0201, USA. FAU - de Almeida, Marcelo N AU - de Almeida MN AUID- ORCID: 0000-0003-3638-7721 AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1134, USA. FAU - Zhang, Jianqiang AU - Zhang J AUID- ORCID: 0000-0001-7228-6202 AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1134, USA. FAU - Gauger, Phillip C AU - Gauger PC AUID- ORCID: 0000-0003-2540-8769 AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011-1134, USA. FAU - Blomme, Allison K AU - Blomme AK AD - Department of Grain Science and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA. FAU - Woodworth, Jason C AU - Woodworth JC AUID- ORCID: 0000-0001-7268-4278 AD - Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA. FAU - Paulk, Chad B AU - Paulk CB AD - Department of Grain Science and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506-0201, USA. FAU - Gebhardt, Jordan T AU - Gebhardt JT AD - Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-0201, USA. LA - eng GR - 20-084; 22-060/National Pork Board/ PT - Journal Article DEP - 20240116 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC10812598 OTO - NOTNLM OT - disinfectants OT - porcine epidemic diarrhea virus OT - porcine reproductive and respiratory syndrome virus OT - swine OT - truck cabs COIS- The authors declare no conflict of interest. EDAT- 2024/01/23 06:43 MHDA- 2024/01/23 06:44 PMCR- 2024/01/16 CRDT- 2024/01/23 01:02 PHST- 2023/12/03 00:00 [received] PHST- 2024/01/07 00:00 [revised] PHST- 2024/01/09 00:00 [accepted] PHST- 2024/01/23 06:44 [medline] PHST- 2024/01/23 06:43 [pubmed] PHST- 2024/01/23 01:02 [entrez] PHST- 2024/01/16 00:00 [pmc-release] AID - ani14020280 [pii] AID - animals-14-00280 [pii] AID - 10.3390/ani14020280 [doi] PST - epublish SO - Animals (Basel). 2024 Jan 16;14(2):280. doi: 10.3390/ani14020280.