PMID- 38256245
OWN - NLM
STAT- MEDLINE
DCOM- 20240124
LR  - 20240202
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 25
IP  - 2
DP  - 2024 Jan 18
TI  - Programmed Death-Ligand (PD-L1), Epidermal Growth Factor (EGF), Relaxin, and 
      Matrix Metalloproteinase-3 (MMP3): Potential Biomarkers of Malignancy in Canine 
      Mammary Neoplasia.
LID - 10.3390/ijms25021170 [doi]
LID - 1170
AB  - Gene expression has been suggested as a putative tool for prognosis and diagnosis 
      in canine mammary neoplasia (CMNs). In the present study, 58 formalin-fixed 
      paraffin-embedded (FFPE) paraffined canine mammary neoplasias from 27 different 
      bitches were included. Thirty-seven tumours were classified as benign, whereas 
      thirty-one were classified as different types of canine carcinoma. In addition, 
      mammary samples from three healthy bitches were also included. The gene 
      expression for vascular endothelial growth factor-alpha (VEGFalpha), CD20, progesterone 
      receptor (PGR), hyaluronidase-1 (HYAL-1), programmed death-ligand 1 (PD-L1), 
      epidermal growth factor (EGF), relaxin (RLN2), and matrix metalloproteinase-3 
      (MMP3) was assessed through RT-qPCR. All the assessed genes yielded a higher 
      expression in neoplastic mammary tissue than in healthy tissue. All the evaluated 
      genes were overexpressed in neoplastic mammary tissue, suggesting a role in the 
      process of tumorigenesis. Moreover, PD-L1, EGF, relaxin, and MMP3 were 
      significantly overexpressed in malignant CMNs compared to benign CMNs, suggesting 
      they may be useful as malignancy biomarkers.
FAU - Galadima, Makchit
AU  - Galadima M
AD  - Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, 
      Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain.
FAU - Teles, Mariana
AU  - Teles M
AD  - Department of Cell Biology, Physiology and Immunology, Universitat Autonoma de 
      Barcelona, 08193 Barcelona, Spain.
FAU - Pastor, Josep
AU  - Pastor J
AD  - Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, 
      Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain.
FAU - Hernandez-Losa, Javier
AU  - Hernandez-Losa J
AUID- ORCID: 0000-0003-1526-3201
AD  - Department of Pathology, Hospital Universitari Vall d'Hebron, VHIR, 08035 
      Barcelona, Spain.
FAU - Rodriguez-Gil, Joan Enric
AU  - Rodriguez-Gil JE
AUID- ORCID: 0000-0002-1112-9884
AD  - Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, 
      Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain.
FAU - Rivera Del Alamo, Maria Montserrat
AU  - Rivera Del Alamo MM
AUID- ORCID: 0000-0002-9557-4888
AD  - Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, 
      Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain.
LA  - eng
PT  - Journal Article
DEP - 20240118
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9002-69-1 (Relaxin)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - 0 (B7-H1 Antigen)
RN  - 0 (Ligands)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Dogs
MH  - Epidermal Growth Factor/genetics
MH  - *Relaxin/genetics
MH  - Matrix Metalloproteinase 3/genetics
MH  - B7-H1 Antigen
MH  - Ligands
MH  - Vascular Endothelial Growth Factor A
MH  - *Mammary Neoplasms, Animal/genetics
MH  - Biomarkers
PMC - PMC10816983
OTO - NOTNLM
OT  - canine mammary neoplasia
OT  - epidermal growth factor
OT  - gene expression
OT  - malignancy biomarkers
OT  - matrix metalloprotease-3
OT  - programmed death-ligand 1
OT  - relaxin
COIS- The authors declare no conflicts of interest.
EDAT- 2024/01/23 06:43
MHDA- 2024/01/24 06:42
PMCR- 2024/01/18
CRDT- 2024/01/23 01:13
PHST- 2023/12/11 00:00 [received]
PHST- 2024/01/08 00:00 [revised]
PHST- 2024/01/16 00:00 [accepted]
PHST- 2024/01/24 06:42 [medline]
PHST- 2024/01/23 06:43 [pubmed]
PHST- 2024/01/23 01:13 [entrez]
PHST- 2024/01/18 00:00 [pmc-release]
AID - ijms25021170 [pii]
AID - ijms-25-01170 [pii]
AID - 10.3390/ijms25021170 [doi]
PST - epublish
SO  - Int J Mol Sci. 2024 Jan 18;25(2):1170. doi: 10.3390/ijms25021170.