PMID- 38256761
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240129
IS  - 2223-7747 (Print)
IS  - 2223-7747 (Electronic)
IS  - 2223-7747 (Linking)
VI  - 13
IP  - 2
DP  - 2024 Jan 11
TI  - Karyotype Variability in Wild Narcissus poeticus L. Populations from Different 
      Environmental Conditions in the Dinaric Alps.
LID - 10.3390/plants13020208 [doi]
LID - 208
AB  - Narcissus poeticus L. (Amaryllidaceae), a facultative serpentinophyte, is a 
      highly variable species and particularly important ancestor of cultivated 
      daffodils, but is rarely studied in field populations. This study, based on 
      natural populations in the Balkans, focused on karyotype variability, genome 
      size, ploidy and the presence of B chromosomes. Thirteen native populations from 
      different environmental and soil conditions were collected and analyzed using 
      flow cytometry to estimate nuclear genome size, fluorescence in situ 
      hybridization (FISH) for physical mapping of rDNA, fluorochrome labeling 
      (chromomycin and Hoechst) for heterochromatin organization and silver nitrate 
      staining of nucleoli for determining rRNA gene activity. The organization of rDNA 
      and natural triploids is reported here for the first time. The presence of 
      individuals with B chromosomes (in 9/13 populations) and chromosomal 
      rearrangements was also detected. The observed B chromosome showed three 
      different morphotypes. The most frequent submetacentric type showed four 
      different patterns, mainly with active ribosomal genes. The results obtained show 
      that N. poeticus has a dynamic genome with variable genome size due to the 
      presence of polyploidy, B chromosomes and chromosomal rearrangements. It is 
      hypothesized that the observed changes reflect the response of the genome to 
      different environmental conditions, where individuals with B chromosomes appear 
      to have certain adaptive advantages.
FAU - Pustahija, Fatima
AU  - Pustahija F
AD  - Faculty of Forestry, University of Sarajevo, Zagrebacka 20, 71000 Sarajevo, 
      Bosnia and Herzegovina.
AD  - Ecologie, Systematique et Evolution, Universite Paris-Sud, CNRS, AgroParisTech, 
      Universite Paris-Saclay, batiment 680-12, route 128, 91190 Gif-sur-Yvette, 
      France.
FAU - Basic, Nedad
AU  - Basic N
AD  - Faculty of Forestry, University of Sarajevo, Zagrebacka 20, 71000 Sarajevo, 
      Bosnia and Herzegovina.
FAU - Siljak-Yakovlev, Sonja
AU  - Siljak-Yakovlev S
AD  - Ecologie, Systematique et Evolution, Universite Paris-Sud, CNRS, AgroParisTech, 
      Universite Paris-Saclay, batiment 680-12, route 128, 91190 Gif-sur-Yvette, 
      France.
LA  - eng
GR  - 983838/NATO - Science For Peace and Security Programme/
GR  - 16214QE/EcoNet project/
PT  - Journal Article
DEP - 20240111
PL  - Switzerland
TA  - Plants (Basel)
JT  - Plants (Basel, Switzerland)
JID - 101596181
PMC - PMC10818684
OTO - NOTNLM
OT  - B chromosomes polymorphism
OT  - chromosome rearrangement
OT  - genome size
OT  - karyotype
OT  - rDNA and heterochromatin organization
COIS- The authors declare no conflicts of interest.
EDAT- 2024/01/23 06:42
MHDA- 2024/01/23 06:43
PMCR- 2024/01/11
CRDT- 2024/01/23 01:16
PHST- 2023/12/01 00:00 [received]
PHST- 2024/01/03 00:00 [revised]
PHST- 2024/01/09 00:00 [accepted]
PHST- 2024/01/23 06:43 [medline]
PHST- 2024/01/23 06:42 [pubmed]
PHST- 2024/01/23 01:16 [entrez]
PHST- 2024/01/11 00:00 [pmc-release]
AID - plants13020208 [pii]
AID - plants-13-00208 [pii]
AID - 10.3390/plants13020208 [doi]
PST - epublish
SO  - Plants (Basel). 2024 Jan 11;13(2):208. doi: 10.3390/plants13020208.