PMID- 38257829
OWN - NLM
STAT- MEDLINE
DCOM- 20240220
LR  - 20240220
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 16
IP  - 1
DP  - 2024 Jan 16
TI  - Chloride Intracellular Channel Protein 1 (CLIC1) Is a Critical Host Cellular 
      Factor for Influenza A Virus Replication.
LID - 10.3390/v16010129 [doi]
LID - 129
AB  - (1) Background: Influenza A Virus (IAV) uses host cellular proteins during 
      replication in host cells. IAV infection causes elevated expression of chloride 
      intracellular channel protein 1 (CLIC1) in lung epithelial cells, but the 
      importance of this protein in IAV replication is unknown. (2) In this study, we 
      determined the role of CLIC1 in IAV replication by investigating the effects of 
      CLIC1 knockdown (KD) on IAV viral protein translation, genomic RNA transcription, 
      and host cellular proteome dysregulation. (3) Results: CLIC1 KD in A549 human 
      lung epithelial cells resulted in a significant decrease in progeny supernatant 
      IAV, but virus protein expression was unaffected. However, a significantly larger 
      number of viral RNAs accumulated in CLIC1 KD cells. Treatment with a CLIC1 
      inhibitor also caused a significant reduction in IAV replication, suggesting that 
      CLIC1 is an important host factor in IAV replication. SomaScan((R)), which measures 
      1322 proteins, identified IAV-induced dysregulated proteins in wild-type cells 
      and in CLIC1 KD cells. The expression of 116 and 149 proteins was significantly 
      altered in wild-type and in CLIC1 KD cells, respectively. A large number of the 
      dysregulated proteins in CLIC1 KD cells were associated with cellular 
      transcription and predicted to be inhibited during IAV replication. (4) 
      Conclusions: This study suggests that CLIC1 is involved in later stages of IAV 
      replication. Further investigation should clarify mechanism(s) for the 
      development of anti-IAV drugs targeting CLIC1 protein.
FAU - Rashid, Mahamud-Ur
AU  - Rashid MU
AUID- ORCID: 0000-0002-3785-2122
AD  - Department of Medical Microbiology and Infectious Diseases, University of 
      Manitoba, Room 543 Basic Medical Sciences Building, 745 Bannatyne Avenue, 
      Winnipeg, MB R3E OJ9, Canada.
AD  - Manitoba Centre for Proteomics and Systems Biology, Room 799, 715 McDermot 
      Avenue, Winnipeg, MB R3E 3P4, Canada.
FAU - Coombs, Kevin M
AU  - Coombs KM
AD  - Department of Medical Microbiology and Infectious Diseases, University of 
      Manitoba, Room 543 Basic Medical Sciences Building, 745 Bannatyne Avenue, 
      Winnipeg, MB R3E OJ9, Canada.
AD  - Manitoba Centre for Proteomics and Systems Biology, Room 799, 715 McDermot 
      Avenue, Winnipeg, MB R3E 3P4, Canada.
AD  - Children's Hospital Research Institute of Manitoba, Room 513, John Buhler 
      Research Centre, 715 McDermot Avenue, Winnipeg, MB R3E 3P4, Canada.
LA  - eng
GR  - MOP-106713/CAPMC/CIHR/Canada
PT  - Journal Article
DEP - 20240116
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Chloride Channels)
RN  - 0 (CLIC1 protein, human)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Humans
MH  - *Chloride Channels/genetics
MH  - *Influenza A virus/physiology
MH  - *Influenza, Human
MH  - RNA, Viral
MH  - *Virus Replication
PMC - PMC10819074
OTO - NOTNLM
OT  - CLIC1
OT  - NPPB
OT  - host factor
OT  - influenza A virus
OT  - replication cycle
OT  - transcription
COIS- The authors declare no conflicts of interest.
EDAT- 2024/01/23 06:43
MHDA- 2024/01/24 06:44
PMCR- 2024/01/16
CRDT- 2024/01/23 01:23
PHST- 2023/11/25 00:00 [received]
PHST- 2024/01/10 00:00 [revised]
PHST- 2024/01/13 00:00 [accepted]
PHST- 2024/01/24 06:44 [medline]
PHST- 2024/01/23 06:43 [pubmed]
PHST- 2024/01/23 01:23 [entrez]
PHST- 2024/01/16 00:00 [pmc-release]
AID - v16010129 [pii]
AID - viruses-16-00129 [pii]
AID - 10.3390/v16010129 [doi]
PST - epublish
SO  - Viruses. 2024 Jan 16;16(1):129. doi: 10.3390/v16010129.