PMID- 38257920
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240129
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 12
IP  - 1
DP  - 2024 Jan 2
TI  - Dissecting the Genetic Basis of the Technological, Functional, and Safety 
      Characteristics of Lacticaseibacillus paracasei SRX10.
LID - 10.3390/microorganisms12010093 [doi]
LID - 93
AB  - Nonstarter lactic acid bacteria (NSLAB) are major contributors to the unique 
      characteristics (e.g., aroma, flavor, texture) of dairy and nondairy fermented 
      products. Lc. paracasei SRX10 is an NSLAB strain originally isolated from a 
      traditional Greek cheese and previously shown to exhibit favorable 
      biotechnological characteristics. More specifically, the strain showed tolerance 
      to simulated gastrointestinal conditions, exopolysaccharide (EPS) biosynthetic 
      capacity, and lack of hemolytic activity and was used in the production of 
      yoghurt and feta cheese with distinct organoleptic characteristics. The aim of 
      the present study was to investigate these traits at the genome level through 
      whole-genome sequencing (WGS), annotation, and comparative genomics. Functional 
      annotation of the genome revealed that Lc. paracasei SRX10 can utilize different 
      carbon sources, leading to the generation of flavor compounds, including lactic 
      acid, acetate, ethanol, and acetoin. Similarly, full clusters for fatty acid 
      biosynthesis, protein and peptide degradation, as well as genes related to 
      survival under extreme temperatures, osmotic shock, and oxidative stress were 
      annotated. Importantly, no transferable antibiotic resistance genes or virulence 
      factors were identified. Finally, strain-specific primers based on genome-wide 
      polymorphisms were designed for the efficient and rapid identification of Lc. 
      paracasei SRX10 via multiplex PCR in fermented products.
FAU - Kamarinou, Christina S
AU  - Kamarinou CS
AUID- ORCID: 0009-0000-9565-3124
AD  - Department of Molecular Biology and Genetics, Faculty of Health Sciences, 
      Democritus University of Thrace, 68100 Alexandroupolis, Greece.
AD  - Institute of Technology of Agricultural Products, Hellenic Agricultural 
      Organization-DIMITRA, 14123 Lycovrissi, Greece.
FAU - Kiousi, Despoina E
AU  - Kiousi DE
AUID- ORCID: 0000-0002-4575-4776
AD  - Department of Molecular Biology and Genetics, Faculty of Health Sciences, 
      Democritus University of Thrace, 68100 Alexandroupolis, Greece.
FAU - Repanas, Panagiotis
AU  - Repanas P
AD  - Department of Molecular Biology and Genetics, Faculty of Health Sciences, 
      Democritus University of Thrace, 68100 Alexandroupolis, Greece.
FAU - Argyri, Anthoula A
AU  - Argyri AA
AUID- ORCID: 0000-0003-3539-8699
AD  - Institute of Technology of Agricultural Products, Hellenic Agricultural 
      Organization-DIMITRA, 14123 Lycovrissi, Greece.
FAU - Chorianopoulos, Nikos G
AU  - Chorianopoulos NG
AD  - Laboratory of Microbiology and Biotechnology of Foods, School of Food and 
      Nutritional Sciences, Department of Food Science and Human Nutrition, 
      Agricultural University of Athens, 11855 Athens, Greece.
FAU - Galanis, Alex
AU  - Galanis A
AUID- ORCID: 0000-0001-9991-3173
AD  - Department of Molecular Biology and Genetics, Faculty of Health Sciences, 
      Democritus University of Thrace, 68100 Alexandroupolis, Greece.
LA  - eng
GR  - 5047285/European Regional Development Fund (ERDF) and national resources 
      (Partnership Agreement 2014-2020)./
PT  - Journal Article
DEP - 20240102
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC10820299
OTO - NOTNLM
OT  - Lacticaseibacillus paracasei
OT  - metabolic capacity
OT  - multiplex PCR
OT  - nonstarter lactic acid bacteria
OT  - whole-genome sequencing
COIS- The authors declare no conflicts of interest.
EDAT- 2024/01/23 06:42
MHDA- 2024/01/23 06:43
PMCR- 2024/01/02
CRDT- 2024/01/23 01:23
PHST- 2023/12/09 00:00 [received]
PHST- 2023/12/30 00:00 [revised]
PHST- 2023/12/31 00:00 [accepted]
PHST- 2024/01/23 06:43 [medline]
PHST- 2024/01/23 06:42 [pubmed]
PHST- 2024/01/23 01:23 [entrez]
PHST- 2024/01/02 00:00 [pmc-release]
AID - microorganisms12010093 [pii]
AID - microorganisms-12-00093 [pii]
AID - 10.3390/microorganisms12010093 [doi]
PST - epublish
SO  - Microorganisms. 2024 Jan 2;12(1):93. doi: 10.3390/microorganisms12010093.