PMID- 38258007 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240129 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 12 IP - 1 DP - 2024 Jan 17 TI - Application of Fourier Transform Infrared Spectroscopy to Discriminate Two Closely Related Bacterial Species: Bacillus anthracis and Bacillus cereus Sensu Stricto. LID - 10.3390/microorganisms12010183 [doi] LID - 183 AB - Fourier transform infrared spectroscopy (FTIRS) is a diagnostic technique historically used in the microbiological field for the characterization of bacterial strains in relation to the specific composition of their lipid, protein, and polysaccharide components. For each bacterial strain, it is possible to obtain a unique absorption spectrum that represents the fingerprint obtained based on the components of the outer cell membrane. In this study, FTIRS was applied for the first time as an experimental diagnostic tool for the discrimination of two pathogenic species belonging to the Bacillus cereus group, Bacillus anthracis and Bacillus cereus sensu stricto; these are two closely related species that are not so easy to differentiate using classical microbiological methods, representing an innovative technology in the field of animal health. FAU - Manzulli, Viviana AU - Manzulli V AUID- ORCID: 0000-0002-8342-5375 AD - Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy. FAU - Cordovana, Miriam AU - Cordovana M AD - Bruker Daltonics GmbH, 28359 Bremen, Germany. FAU - Serrecchia, Luigina AU - Serrecchia L AD - Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy. FAU - Rondinone, Valeria AU - Rondinone V AUID- ORCID: 0000-0001-7842-8697 AD - Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy. FAU - Pace, Lorenzo AU - Pace L AUID- ORCID: 0000-0003-0742-5119 AD - Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy. FAU - Farina, Donatella AU - Farina D AD - Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy. FAU - Cipolletta, Dora AU - Cipolletta D AD - Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy. FAU - Caruso, Marta AU - Caruso M AD - Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy. FAU - Fraccalvieri, Rosa AU - Fraccalvieri R AUID- ORCID: 0000-0001-9261-7128 AD - Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy. FAU - Difato, Laura Maria AU - Difato LM AD - Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy. FAU - Tolve, Francesco AU - Tolve F AD - Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy. FAU - Vetritto, Valerio AU - Vetritto V AD - Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy. FAU - Galante, Domenico AU - Galante D AUID- ORCID: 0000-0002-1159-2801 AD - Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, 71121 Foggia, Italy. LA - eng PT - Journal Article DEP - 20240117 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC10821103 OTO - NOTNLM OT - Bacillus anthracis OT - Bacillus cereus sensu stricto OT - FTIR-spectroscopy OT - IR Biotyper COIS- Miriam Cordovana is an employee of the company Bruker Daltonic GmbH. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2024/01/23 06:43 MHDA- 2024/01/23 06:44 PMCR- 2024/01/17 CRDT- 2024/01/23 01:24 PHST- 2023/11/27 00:00 [received] PHST- 2023/12/29 00:00 [revised] PHST- 2024/01/12 00:00 [accepted] PHST- 2024/01/23 06:44 [medline] PHST- 2024/01/23 06:43 [pubmed] PHST- 2024/01/23 01:24 [entrez] PHST- 2024/01/17 00:00 [pmc-release] AID - microorganisms12010183 [pii] AID - microorganisms-12-00183 [pii] AID - 10.3390/microorganisms12010183 [doi] PST - epublish SO - Microorganisms. 2024 Jan 17;12(1):183. doi: 10.3390/microorganisms12010183.