PMID- 38258516
OWN - NLM
STAT- MEDLINE
DCOM- 20240124
LR  - 20240202
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 33
DP  - 2024 Jan-Dec
TI  - Effect of High BMI on Human Bone Marrow-Derived Mesenchymal Stromal Cells.
PG  - 9636897241226546
LID - 10.1177/09636897241226546 [doi]
LID - 09636897241226546
AB  - Bone marrow-derived mesenchymal stromal cells (BMSCs) are attractive candidates 
      in tissue engineering and regenerative medicine. Growing evidence has suggested 
      that a high body mass index (BMI) can affect the properties of BMSCs, resulting 
      in a reduced quality of the cells. However, the results are not consistent. 
      Therefore, this study aimed to investigate the influences of high BMI on human 
      BMSCs (hBMSCs). To avoid gender bias, BMSCs from females and males were studied 
      independently. Finally, hBMSCs from 89 females and 152 males were separately 
      divided into the normal BMI group (18.5 kg/m(2) </= BMI < 25 kg/m(2)) and the high 
      BMI group (BMI > 25 kg/m(2)). The cells were analyzed for the colony-forming 
      potential; proliferation capacity; in vitro adipogenic, osteogenic, and 
      chondrogenic differentiation potentials; and the expression of 32 common surface 
      antigens. The results showed that high BMI did not change the number of colonies 
      at passage 1 in females and males. In contrast, significantly reduced colony 
      numbers at passage 4 (P4) were found in both female and male donors with high 
      BMI. The doubling time of hBMSCs was comparable between the normal and the high 
      BMI groups of females and males. Furthermore, the results of trilineage 
      differentiation did not differ between the different BMI groups of males. In 
      females, the high and the normal BMI groups also showed similar adipogenic and 
      chondrogenic differentiation, while osteogenic differentiation was significantly 
      enhanced in the high-BMI group. Regarding the expression of surface antigens, the 
      expressions of CD200 and SSEA4 on hBMSCs were reduced in the high-BMI group of 
      females and males, respectively. In conclusion, high BMI suppressed the 
      clonogenicity of female and male hBMSCs at P4, improved the in vitro osteogenesis 
      of female hBMSCs, and decreased the expressions of CD200 on hBMSCs in females and 
      SSEA4 in males.
FAU - Zong, Qiang
AU  - Zong Q
AD  - Department of Trauma Surgery, Hannover Medical School, Hannover, Germany.
FAU - Bundkirchen, Katrin
AU  - Bundkirchen K
AD  - Department of Trauma Surgery, Hannover Medical School, Hannover, Germany.
FAU - Neunaber, Claudia
AU  - Neunaber C
AD  - Department of Trauma Surgery, Hannover Medical School, Hannover, Germany.
FAU - Noack, Sandra
AU  - Noack S
AUID- ORCID: 0000-0002-5880-7611
AD  - Department of Trauma Surgery, Hannover Medical School, Hannover, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Antigens, Surface)
SB  - IM
MH  - Humans
MH  - Female
MH  - Male
MH  - Body Mass Index
MH  - *Bone Marrow
MH  - Osteogenesis
MH  - Sexism
MH  - Antigens, Surface
MH  - *Mesenchymal Stem Cells
PMC - PMC10807335
OTO - NOTNLM
OT  - bone marrow-derived mesenchymal stromal cells
OT  - high BMI
OT  - obesity
OT  - overweight
COIS- Declaration of Conflicting InterestsThe author(s) declared no potential conflicts 
      of interest with respect to the research, authorship, and/or publication of this 
      article.
EDAT- 2024/01/23 06:42
MHDA- 2024/01/24 06:42
PMCR- 2024/01/23
CRDT- 2024/01/23 04:23
PHST- 2024/01/24 06:42 [medline]
PHST- 2024/01/23 06:42 [pubmed]
PHST- 2024/01/23 04:23 [entrez]
PHST- 2024/01/23 00:00 [pmc-release]
AID - 10.1177_09636897241226546 [pii]
AID - 10.1177/09636897241226546 [doi]
PST - ppublish
SO  - Cell Transplant. 2024 Jan-Dec;33:9636897241226546. doi: 
      10.1177/09636897241226546.