PMID- 38260733
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240124
IS  - 2296-4185 (Print)
IS  - 2296-4185 (Electronic)
IS  - 2296-4185 (Linking)
VI  - 11
DP  - 2023
TI  - Pulmonary decellularized extracellular matrix (dECM) modified polyethylene 
      terephthalate three-dimensional cell carriers regulate the proliferation and 
      paracrine activity of mesenchymal stem cells.
PG  - 1324424
LID - 10.3389/fbioe.2023.1324424 [doi]
LID - 1324424
AB  - Introduction: Mesenchymal stem cells (MSCs) possess a high degree of self-renewal 
      capacity and in vitro multi-lineage differentiation potential. Decellularized 
      materials have garnered considerable attention due to their elevated 
      biocompatibility, reduced immunogenicity, excellent biodegradability, and the 
      ability to partially mimic the in vivo microenvironment conducive to cell growth. 
      To address the issue of mesenchymal stem cells losing their stem cell 
      characteristics during two-dimensional (2D) cultivation, this study established 
      three-dimensional cell carriers modified with lung decellularized extracellular 
      matrix and assessed its impact on the life activities of mesenchymal stem cells. 
      Methods: This study employed PET as a substrate material, grafting with 
      polydopamine (PDA), and constructing a decellularized extracellular matrix (dECM) 
      coating on its surface, thus creating the PET/PDA/dECM three-dimensional (3D) 
      composite carrier. Subsequently, material characterization of the cellular 
      carriers was conducted, followed by co-culturing with human umbilical cord 
      mesenchymal stem cells in vitro, aiming to investigate the material's impact on 
      the proliferation and paracrine activity of mesenchymal stem cells. Results and 
      Discussion: Material characterization demonstrated successful grafting of PDA and 
      dECM materials, and it had complete hydrophilicity, high porosity, and excellent 
      mechanical properties. The material was rich in various ECM proteins (collagen I, 
      collagen IV , laminin, fibronectin, elastin), indicating good biocompatibility. 
      In long-term in vitro cultivation (14 days) experiments, the PET/PDA/dECM 
      three-dimensional composite carrier significantly enhanced adhesion and 
      proliferation of human umbilical cord-derived mesenchymal stem cells (HUCMSCs), 
      with a proliferation rate 1.9 times higher than that of cells cultured on tissue 
      culture polystyrene (TCPS) at day 14. Furthermore, it effectively maintained the 
      stem cell characteristics, expressing specific antigens for HUCMSCs. Through 
      qPCR, Western blot, and ELISA experiments, the composite carrier markedly 
      promoted the expression and secretion of key cell factors in HUCMSCs. These 
      results demonstrate that the PET/PDA/dECM composite carrier holds great potential 
      for scaling up MSCs' long-term in vitro cultivation and the production of 
      paracrine factors.
CI  - Copyright (c) 2024 Li, Zhang, Ye, Wang, Ouyang, Luo and Gong.
FAU - Li, Jinze
AU  - Li J
AD  - School of Biomedical Engineering, Shenzhen Campus of Sun Yat-Sen University, 
      Shenzhen, China.
FAU - Zhang, Jiali
AU  - Zhang J
AD  - School of Biomedical Engineering, Shenzhen Campus of Sun Yat-Sen University, 
      Shenzhen, China.
FAU - Ye, Hao
AU  - Ye H
AD  - School of Biomedical Engineering, Shenzhen Campus of Sun Yat-Sen University, 
      Shenzhen, China.
FAU - Wang, Qixuan
AU  - Wang Q
AD  - School of Biomedical Engineering, Shenzhen Campus of Sun Yat-Sen University, 
      Shenzhen, China.
FAU - Ouyang, Yanran
AU  - Ouyang Y
AD  - School of Biomedical Engineering, Shenzhen Campus of Sun Yat-Sen University, 
      Shenzhen, China.
FAU - Luo, Yuxi
AU  - Luo Y
AD  - School of Biomedical Engineering, Shenzhen Campus of Sun Yat-Sen University, 
      Shenzhen, China.
AD  - Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical 
      Instrument, Sun Yat-Sen University, Guangzhou, China.
FAU - Gong, Yihong
AU  - Gong Y
AD  - School of Biomedical Engineering, Shenzhen Campus of Sun Yat-Sen University, 
      Shenzhen, China.
AD  - Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical 
      Instrument, Sun Yat-Sen University, Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20240108
PL  - Switzerland
TA  - Front Bioeng Biotechnol
JT  - Frontiers in bioengineering and biotechnology
JID - 101632513
PMC - PMC10800494
OTO - NOTNLM
OT  - decellularized materials
OT  - extracellular matrix
OT  - growth factor expression
OT  - mesenchymal stem cells
OT  - tissue engineering
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2024/01/23 12:43
MHDA- 2024/01/23 12:44
PMCR- 2023/01/01
CRDT- 2024/01/23 10:39
PHST- 2023/10/19 00:00 [received]
PHST- 2023/12/07 00:00 [accepted]
PHST- 2024/01/23 12:44 [medline]
PHST- 2024/01/23 12:43 [pubmed]
PHST- 2024/01/23 10:39 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 1324424 [pii]
AID - 10.3389/fbioe.2023.1324424 [doi]
PST - epublish
SO  - Front Bioeng Biotechnol. 2024 Jan 8;11:1324424. doi: 10.3389/fbioe.2023.1324424. 
      eCollection 2023.