PMID- 38261086 OWN - NLM STAT- MEDLINE DCOM- 20240124 LR - 20240202 IS - 1573-4978 (Electronic) IS - 0301-4851 (Linking) VI - 51 IP - 1 DP - 2024 Jan 23 TI - Evaluation of the effect of mitoquinone on functional parameters, DNA structure, and genes expression related to the apoptotic and antioxidants of human sperm after freezing-thawing. PG - 183 LID - 10.1007/s11033-023-09020-0 [doi] AB - OBJECTIVE: Sperm freezing is considered as an effective way in assisted reproductive technology (ART) programs, it has detrimental effects on sperm function, due to the production of reactive oxygen species (ROS). This study aimed to investigate the potential of Mitoquinone (MitoQ) in inhibiting the production of mitochondrial ROS during sperm freezing. METHODS: A total of 20 human normozoosperm samples were collected for this study. The samples were divided into four groups, each containing different concentrations of MitoQ (0, 0.2, 2, and 20 nM), and then subjected to the freezing process. After thawing, the sperm suspensions were evaluated for parameters including motility, morphology, acrosome integrity, adenosine triphosphate (ATP) level, intracellular ROS, viability, chromatin packaging, DNA denaturation, DNA fragmentation, as well as the expression of antioxidants (GPX, SOD) and apoptotic (Bax, Bcl(2)) genes. RESULTS: The results showed that total and progressive mobility of sperms significantly increased in the 2 nM group, while significantly decreased in the 20 nM group (p /= 0.05). Intracellular ROS levels showed a significant decrease and increase in the concentrations of 2 and 20 nM, respectively (p /= 0.05). All the comparisons were made with respect to the control group. CONCLUSION: Adding the optimal concentration of MitoQ (2 nM) to the sperm freezing medium not only improves sperm functional parameters and reduces DNA damages, but also stimulates the expression of antioxidant genes, leading to even greater benefits for sperm cryopreservation. CI - (c) 2024. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Moradi Gardeshi, Tohid AU - Moradi Gardeshi T AUID- ORCID: 0000-0002-3366-0211 AD - Department of Veterinary Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran. FAU - Shahandeh, Erfan AU - Shahandeh E AUID- ORCID: 0000-0002-7004-4514 AD - Department of Biology, Islamic Azad University of Hamedan, Hamadan, Iran. FAU - Tavakolpoor Saleh, Nafiseh AU - Tavakolpoor Saleh N AUID- ORCID: 0000-0002-1821-6294 AD - Department of Biophysics and Biochemistry, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. FAU - Karami, Somayeh AU - Karami S AUID- ORCID: 0000-0002-7460-2536 AD - Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. FAU - Mirzaei Azandaryani, Zahra AU - Mirzaei Azandaryani Z AUID- ORCID: 0000-0002-3457-9271 AD - Department of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran. FAU - Mazaheri, Fahime AU - Mazaheri F AUID- ORCID: 0000-0002-2066-2656 AD - Research and Clinical Center of Infertility, Yazd Reproductive Science Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. FAU - Mohammadi, Hiwa AU - Mohammadi H AUID- ORCID: 0000-0003-2421-3396 AD - Social Determinants of Health Research CenterBoroujerd School of Nursing, Lorestan University of Medical Sciences, Khorramabad, Iran. hiva.nursing@gmail.com. LA - eng PT - Journal Article DEP - 20240123 PL - Netherlands TA - Mol Biol Rep JT - Molecular biology reports JID - 0403234 RN - 0 (Antioxidants) RN - 47BYS17IY0 (mitoquinone) RN - 0 (Reactive Oxygen Species) RN - 9007-49-2 (DNA) RN - 0 (Chromatin) RN - 8L70Q75FXE (Adenosine Triphosphate) RN - 0 (Organophosphorus Compounds) RN - 1339-63-5 (Ubiquinone) SB - IM MH - Male MH - Humans MH - *Antioxidants/pharmacology MH - Freezing MH - Reactive Oxygen Species MH - *Semen MH - DNA MH - Spermatozoa MH - Chromatin MH - Adenosine Triphosphate MH - *Organophosphorus Compounds MH - Ubiquinone/*analogs & derivatives OTO - NOTNLM OT - Chromatin packaging OT - DNA fragmentation OT - Gene expression OT - Human sperm EDAT- 2024/01/23 12:43 MHDA- 2024/01/24 06:43 CRDT- 2024/01/23 11:07 PHST- 2023/10/05 00:00 [received] PHST- 2023/11/26 00:00 [accepted] PHST- 2024/01/24 06:43 [medline] PHST- 2024/01/23 12:43 [pubmed] PHST- 2024/01/23 11:07 [entrez] AID - 10.1007/s11033-023-09020-0 [pii] AID - 10.1007/s11033-023-09020-0 [doi] PST - epublish SO - Mol Biol Rep. 2024 Jan 23;51(1):183. doi: 10.1007/s11033-023-09020-0.