PMID- 38266619
OWN - NLM
STAT- MEDLINE
DCOM- 20240214
LR  - 20240214
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 250
DP  - 2024 Apr 15
TI  - Microcavity well-plate for automated parallel bioelectronic analysis of 3D cell 
      cultures.
PG  - 116042
LID - S0956-5663(24)00045-9 [pii]
LID - 10.1016/j.bios.2024.116042 [doi]
AB  - Three-dimensional (3D) in vitro cell culture models serve as valuable tools for 
      accurately replicating cellular microenvironments found in vivo. While cell 
      culture technologies are rapidly advancing, the availability of non-invasive, 
      real-time, and label-free analysis methods for 3D cultures remains limited. To 
      meet the demand for higher-throughput drug screening, there is a demanding need 
      for analytical methods that can operate in parallel. Microelectrode systems in 
      combination with microcavity arrays (MCAs), offer the capability of spatially 
      resolved electrochemical impedance analysis and field potential monitoring of 3D 
      cultures. However, the fabrication and handling of small-scale MCAs have been 
      labour-intensive, limiting their broader application. To overcome this challenge, 
      we have established a process for creating MCAs in a standard 96-well plate 
      format using high-precision selective laser etching. In addition, to automate and 
      ensure the accurate placement of 3D cultures on the MCA, we have designed and 
      characterized a plug-in tool using SLA-3D-printing. To characterize our new 
      96-well plate MCA-based platform, we conducted parallel analyses of human 
      melanoma 3D cultures and monitored the effect of cisplatin in real-time by 
      impedance spectroscopy. In the following we demonstrate the capabilities of the 
      MCA approach by analysing contraction rates of human pluripotent stem 
      cell-derived cardiomyocyte aggregates in response to cardioactive compounds. In 
      summary, our MCA system significantly expands the possibilities for label-free 
      analysis of 3D cell and tissue cultures, offering an order of magnitude higher 
      parallelization capacity than previous devices. This advancement greatly enhances 
      its applicability in real-world settings, such as drug development or clinical 
      diagnostics.
CI  - Copyright (c) 2024 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Zitzmann, Franziska D
AU  - Zitzmann FD
AD  - Centre for Biotechnology and Biomedicine, Biochemical Cell Technology, Leipzig 
      University, Deutscher Platz 5, D-04103, Leipzig, Germany; b-ACT Matter, Research 
      and Transfer Centre for bioactive Matter, Leipzig University, Deutscher Platz 5, 
      D-04103, Leipzig, Germany.
FAU - Schmidt, Sabine
AU  - Schmidt S
AD  - Centre for Biotechnology and Biomedicine, Biochemical Cell Technology, Leipzig 
      University, Deutscher Platz 5, D-04103, Leipzig, Germany.
FAU - Frank, Ronny
AU  - Frank R
AD  - Centre for Biotechnology and Biomedicine, Biochemical Cell Technology, Leipzig 
      University, Deutscher Platz 5, D-04103, Leipzig, Germany.
FAU - Weigel, Winnie
AU  - Weigel W
AD  - Centre for Biotechnology and Biomedicine, Biochemical Cell Technology, Leipzig 
      University, Deutscher Platz 5, D-04103, Leipzig, Germany.
FAU - Meier, Matthias
AU  - Meier M
AD  - Centre for Biotechnology and Biomedicine, Biochemical Cell Technology, Leipzig 
      University, Deutscher Platz 5, D-04103, Leipzig, Germany; Helmholtz Pioneer 
      Campus, Helmholtz Zentrum Munich, Neuherberg, Germany.
FAU - Jahnke, Heinz-Georg
AU  - Jahnke HG
AD  - Centre for Biotechnology and Biomedicine, Biochemical Cell Technology, Leipzig 
      University, Deutscher Platz 5, D-04103, Leipzig, Germany. Electronic address: 
      heinz-georg.jahnke@bbz.uni-leipzig.de.
LA  - eng
PT  - Journal Article
DEP - 20240114
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
SB  - IM
MH  - Humans
MH  - *Biosensing Techniques
MH  - Myocytes, Cardiac
MH  - Cell Culture Techniques/methods
MH  - Cell Culture Techniques, Three Dimensional
MH  - Dielectric Spectroscopy
OTO - NOTNLM
OT  - 3D cell culture
OT  - Contractile human cardiomyocyte clusters
OT  - Electrochemical impedance spectroscopy
OT  - Field potential monitoring
OT  - Microcavity array technology
OT  - Selective laser etching
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/01/25 00:42
MHDA- 2024/02/12 05:43
CRDT- 2024/01/24 18:35
PHST- 2023/10/31 00:00 [received]
PHST- 2024/01/10 00:00 [revised]
PHST- 2024/01/12 00:00 [accepted]
PHST- 2024/02/12 05:43 [medline]
PHST- 2024/01/25 00:42 [pubmed]
PHST- 2024/01/24 18:35 [entrez]
AID - S0956-5663(24)00045-9 [pii]
AID - 10.1016/j.bios.2024.116042 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2024 Apr 15;250:116042. doi: 10.1016/j.bios.2024.116042. 
      Epub 2024 Jan 14.