PMID- 38270683
OWN - NLM
STAT- MEDLINE
DCOM- 20240126
LR  - 20240206
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 51
IP  - 1
DP  - 2024 Jan 25
TI  - Inhibitory effect of rosmarinic acid on IgE-trigged mast cell degranulation in 
      vitro and in vivo.
PG  - 194
LID - 10.1007/s11033-023-09164-z [doi]
AB  - BACKGROUND: Rosmarinic acid (RA), a polyphenol from edible-medical Lamiaceae 
      herbs, is known to possess a variety of pharmacological activity, like 
      anti-inflammatory, hepatoprotective and immunoregulation activities. METHODS AND 
      RESULTS: Hereon, we investigated the anti-allergic activity of RA on 
      immunoglobulin E (IgE)-mediated anaphylaxis responses in rat basophilic leukemia 
      (RBL)-2H3 mast cell. RA hindered the morphological changes of IgE-induced 
      degranulated RBL-2H3 cells. The release of two key biomarkers (beta-hexosaminidase 
      (beta-HEX) and histamine) of IgE-induced degranulated mast cells was also remarkably 
      down-regulated by RA intervention in a dose dependent manner. Moreover, RA 
      inhibited IgE-induced ROS overproduction and flux of intracellular Ca(2+) in 
      IgE-mediated degranulated mast cells. The q-PCR analysis showed that the 
      expressions of genes (COX 2, PGD 2, LTC 4, HDC, Nrf2, HO-1 and NQO1) involved in 
      MAPK and oxidative stress signaling pathways were significantly regulated by RA 
      intervention. Moreover, the degranulation inhibitory effect of rosmarinic acid 
      was investigated on the anti-DNP IgE/DNP-HSA induced passive cutaneous 
      anaphylaxis (PCA) mice model in vivo. It showed that RA significantly inhibited 
      the PCA reaction and allergic edema of ears in anti-DNP IgE/DNP-HSA stimulated 
      mice. CONCLUSION: These findings suggest that RA has the potential to be used as 
      a therapeutic candidate for allergic diseases by inhibiting mast cell 
      degranulation. This indicates a possible role for RA in managing allergic 
      reactions and related conditions.
CI  - (c) 2024. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Jia, Binmei
AU  - Jia B
AD  - Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, 
      Wuhan Polytechnic University, Wuhan, 430023, China.
AD  - Food Safety Research Center, Key Research Institute of Humanities and Social of 
      Hubei Province, Wuhan, 430023, China.
FAU - Zeng, Hao-Long
AU  - Zeng HL
AD  - Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430030, China.
FAU - Shang, Jieli
AU  - Shang J
AD  - Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, 
      Wuhan Polytechnic University, Wuhan, 430023, China.
AD  - Food Safety Research Center, Key Research Institute of Humanities and Social of 
      Hubei Province, Wuhan, 430023, China.
FAU - Wang, Xuanpei
AU  - Wang X
AD  - Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, 
      Wuhan Polytechnic University, Wuhan, 430023, China.
AD  - Food Safety Research Center, Key Research Institute of Humanities and Social of 
      Hubei Province, Wuhan, 430023, China.
FAU - Xu, Lin
AU  - Xu L
AD  - Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, 
      Wuhan Polytechnic University, Wuhan, 430023, China.
AD  - Food Safety Research Center, Key Research Institute of Humanities and Social of 
      Hubei Province, Wuhan, 430023, China.
FAU - Fang, Min
AU  - Fang M
AD  - Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, 
      Wuhan Polytechnic University, Wuhan, 430023, China.
AD  - Food Safety Research Center, Key Research Institute of Humanities and Social of 
      Hubei Province, Wuhan, 430023, China.
FAU - Zeng, Fengbo
AU  - Zeng F
AD  - Wuhan BioCSi Tech Laboratory Co., LTD, Wuhan, 430000, China.
FAU - Yang, Qing
AU  - Yang Q
AD  - Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, 
      Wuhan Polytechnic University, Wuhan, 430023, China. qingyang@whu.edu.cn.
AD  - Food Safety Research Center, Key Research Institute of Humanities and Social of 
      Hubei Province, Wuhan, 430023, China. qingyang@whu.edu.cn.
LA  - eng
GR  - NJZ2022009/the Open Research Fund Program of Key Laboratory for Deep Processing 
      of Major Grain and Oil, Ministry of Education, China/
PT  - Journal Article
DEP - 20240125
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - MQE6XG29YI (Rosmarinic Acid)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Mice
MH  - *Mast Cells
MH  - Rosmarinic Acid
MH  - Cell Degranulation
MH  - *Hypersensitivity
MH  - Immunoglobulin E
OTO - NOTNLM
OT  - Intracellular Ca2+
OT  - PCA
OT  - RBL-2H3 cells
OT  - ROS
OT  - Rosmarinic acid
EDAT- 2024/01/25 12:42
MHDA- 2024/01/26 06:44
CRDT- 2024/01/25 11:07
PHST- 2023/08/07 00:00 [received]
PHST- 2023/12/14 00:00 [accepted]
PHST- 2024/01/26 06:44 [medline]
PHST- 2024/01/25 12:42 [pubmed]
PHST- 2024/01/25 11:07 [entrez]
AID - 10.1007/s11033-023-09164-z [pii]
AID - 10.1007/s11033-023-09164-z [doi]
PST - epublish
SO  - Mol Biol Rep. 2024 Jan 25;51(1):194. doi: 10.1007/s11033-023-09164-z.