PMID- 38271377
OWN - NLM
STAT- MEDLINE
DCOM- 20240129
LR  - 20240206
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 19
IP  - 1
DP  - 2024
TI  - Prenatal alcohol exposure alters expression of genes involved in cell adhesion, 
      immune response, and toxin metabolism in adolescent rat hippocampus.
PG  - e0293425
LID - 10.1371/journal.pone.0293425 [doi]
LID - e0293425
AB  - Prenatal alcohol exposure (PAE) can result in mild to severe consequences for 
      children throughout their lives, with this range of symptoms referred to as Fetal 
      Alcohol Spectrum Disorders (FASD). These consequences are thought to be linked to 
      changes in gene expression and transcriptional programming in the brain, but the 
      identity of those changes, and how they persist into adolescence are unclear. In 
      this study, we isolated RNA from the hippocampus of adolescent rats exposed to 
      ethanol during prenatal development and compared gene expression to controls. 
      Briefly, dams were either given free access to standard chow ad libitum (AD), 
      pair-fed a liquid diet (PF) or were given a liquid diet with ethanol (6.7% 
      ethanol, ET) throughout gestation (gestational day (GD) 0-20). All dams were 
      given control diet ad libitum beginning on GD 20 and throughout parturition and 
      lactation. Hippocampal tissue was collected from adolescent male and female 
      offspring (postnatal day (PD) 35-36). Exposure to ethanol caused widespread 
      downregulation of many genes as compared to control rats. Gene ontology analysis 
      demonstrated that affected pathways included cell adhesion, toxin metabolism, and 
      immune responses. Interestingly, these differences were not strongly affected by 
      sex. Furthermore, these changes were consistent when comparing ethanol-exposed 
      rats to pair-fed controls provided with a liquid diet and those fed ad libitum on 
      a standard chow diet. We conclude from this study that changes in genetic 
      architecture and the resulting neuronal connectivity after prenatal exposure to 
      alcohol continue through adolescent development. Further research into the 
      consequences of specific gene expression changes on neural and behavioral changes 
      will be vital to our understanding of the FASD spectrum of diseases.
CI  - Copyright: (c) 2024 Khalifa et al. This is an open access article distributed under 
      the terms of the Creative Commons Attribution License, which permits unrestricted 
      use, distribution, and reproduction in any medium, provided the original author 
      and source are credited.
FAU - Khalifa, Amal
AU  - Khalifa A
AD  - Department of Computer Science, Purdue University Fort Wayne, Fort Wayne, IN, 
      United States of America.
FAU - Palu, Rebecca
AU  - Palu R
AUID- ORCID: 0000-0001-9444-8815
AD  - Department of Biological Sciences, Purdue University Fort Wayne, Fort Wayne, IN, 
      United States of America.
FAU - Perkins, Amy E
AU  - Perkins AE
AD  - Department of Psychology, Purdue University Fort Wayne, Fort Wayne, IN, United 
      States of America.
FAU - Volz, Avery
AU  - Volz A
AUID- ORCID: 0009-0003-6546-4260
AD  - Department of Biological Sciences, Purdue University Fort Wayne, Fort Wayne, IN, 
      United States of America.
AD  - Department of Psychology, Purdue University Fort Wayne, Fort Wayne, IN, United 
      States of America.
LA  - eng
PT  - Journal Article
DEP - 20240125
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Humans
MH  - Child
MH  - Rats
MH  - Female
MH  - Male
MH  - Pregnancy
MH  - Animals
MH  - Adolescent
MH  - *Fetal Alcohol Spectrum Disorders/genetics/metabolism
MH  - *Prenatal Exposure Delayed Effects/genetics/metabolism
MH  - Cell Adhesion
MH  - Hippocampus/metabolism
MH  - Ethanol/toxicity/metabolism
MH  - Parturition
MH  - Immunity
PMC - PMC10810486
COIS- The authors have declared that no competing interests exist.
EDAT- 2024/01/25 18:42
MHDA- 2024/01/29 06:42
PMCR- 2024/01/25
CRDT- 2024/01/25 13:42
PHST- 2023/06/15 00:00 [received]
PHST- 2023/10/11 00:00 [accepted]
PHST- 2024/01/29 06:42 [medline]
PHST- 2024/01/25 18:42 [pubmed]
PHST- 2024/01/25 13:42 [entrez]
PHST- 2024/01/25 00:00 [pmc-release]
AID - PONE-D-23-18670 [pii]
AID - 10.1371/journal.pone.0293425 [doi]
PST - epublish
SO  - PLoS One. 2024 Jan 25;19(1):e0293425. doi: 10.1371/journal.pone.0293425. 
      eCollection 2024.