PMID- 38274830
OWN - NLM
STAT- MEDLINE
DCOM- 20240129
LR  - 20240406
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 14
DP  - 2023
TI  - HLA-DPB1 genotype variants predict DP molecule cell surface expression and DP 
      donor specific antibody binding capacity.
PG  - 1328533
LID - 10.3389/fimmu.2023.1328533 [doi]
LID - 1328533
AB  - The contribution of alloresponses to mismatched HLA-DP in solid organ 
      transplantation and hematopoietic stem cell transplantation (HCT) has been well 
      documented. Exploring the regulatory mechanisms of DPB1 alleles has become an 
      important question to be answered. In this study, our initial investigation 
      focused on examining the correlation between the rs9277534G/A SNP and DPB1 mRNA 
      expression. The result showed that there was a significant increase in DPB1 mRNA 
      expression in B lymphoblastoid cell lines (BLCLs) with the rs9277534GG genotype 
      compared to rs9277534AA genotype. In addition, B cells with the rs9277534GG 
      exhibited significantly higher DP protein expression than those carrying the 
      rs9277534AA genotype in primary B cells. Furthermore, we observed a significant 
      upregulation of DP expression in B cells following treatment with Interleukin 13 
      (IL-13) compared to untreated B cells carrying rs9277534GG-linked DPB1 alleles. 
      Fluorescence in situ hybridization (FISH) analysis of DPB1 in BLCL demonstrated 
      significant differences in both the cytoplasmic (p=0.0003) and nuclear (p=0.0001) 
      localization of DP mRNA expression comparing DPB1*04:01 (rs9277534AA) and 
      DPB1*05:01 (rs9277534GG) homozygous cells. The study of the correlation between 
      differential DPB1 expression and long non-coding RNAs (lncRNAs) showed that 
      lnc-HLA-DPB1-13:1 is strongly associated with DP expression (r=0.85), suggesting 
      the potential involvement of lncRNA in regulating DP expression. The correlation 
      of DP donor specific antibody (DSA) with B cell flow crossmatch (B-FCXM) results 
      showed a better linear correlation of DP DSA against GG and AG donor cells (R(2) 
      = 0.4243, p=0.0025 and R(2) = 0.6172, p=0.0003, respectively), compared to DSA 
      against AA donor cells (R(2) = 0.0649, p=0.4244). This explained why strong DP 
      DSA with a low expression DP leads to negative B-FCXM. In conclusion, this study 
      provides evidence supporting the involvement of lncRNA in modulating HLA-DP 
      expression, shedding lights on the intricate regulatory mechanisms of DP, 
      particularly under inflammatory conditions in transplantation.
CI  - Copyright (c) 2024 Yin, Soe, Valenzuela, Reed and Zhang.
FAU - Yin, Yuxin
AU  - Yin Y
AD  - UCLA Immunogenetics Center, Department of Pathology & Laboratory Medicine, Los 
      Angeles, CA, United States.
FAU - Soe, Nwe Nwe
AU  - Soe NN
AD  - Department of Pathology, AdventHealth Tissue Typing Laboratory, Orlando, FL, 
      United States.
FAU - Valenzuela, Nicole M
AU  - Valenzuela NM
AD  - UCLA Immunogenetics Center, Department of Pathology & Laboratory Medicine, Los 
      Angeles, CA, United States.
FAU - Reed, Elaine F
AU  - Reed EF
AD  - UCLA Immunogenetics Center, Department of Pathology & Laboratory Medicine, Los 
      Angeles, CA, United States.
FAU - Zhang, Qiuheng
AU  - Zhang Q
AD  - UCLA Immunogenetics Center, Department of Pathology & Laboratory Medicine, Los 
      Angeles, CA, United States.
LA  - eng
PT  - Journal Article
DEP - 20240111
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (HLA-DPB1 antigen)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (HLA-DP beta-Chains)
RN  - 0 (Antibodies)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *RNA, Long Noncoding
MH  - HLA-DP beta-Chains/genetics
MH  - Genotype
MH  - Antibodies/genetics
MH  - Unrelated Donors
MH  - RNA, Messenger
PMC - PMC10808447
OTO - NOTNLM
OT  - HLA-DPB1
OT  - crossmatch
OT  - hematopoietic stem cell transplantation
OT  - long non-coding RNA
OT  - rs9277534
OT  - transplantation
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2024/01/26 06:44
MHDA- 2024/01/29 06:44
PMCR- 2023/01/01
CRDT- 2024/01/26 03:59
PHST- 2023/10/26 00:00 [received]
PHST- 2023/12/21 00:00 [accepted]
PHST- 2024/01/29 06:44 [medline]
PHST- 2024/01/26 06:44 [pubmed]
PHST- 2024/01/26 03:59 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2023.1328533 [doi]
PST - epublish
SO  - Front Immunol. 2024 Jan 11;14:1328533. doi: 10.3389/fimmu.2023.1328533. 
      eCollection 2023.