PMID- 38275403
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240128
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 12
IP  - 1
DP  - 2024 Jan 19
TI  - Novel Serum Biomarkers for Patients with Allergic Asthma Phenotype.
LID - 10.3390/biomedicines12010232 [doi]
LID - 232
AB  - In distinguishing the allergic asthma (AA) phenotype, it has been identified that 
      specific biomarkers could assist; however, none of them are considered ideal. 
      This study aimed to analyze three groups of biologically active substances in the 
      serum. Twenty steroid-free AA patients, sensitized to Dermatophagoides 
      pteronyssinus, and sixteen healthy subjects (HSs) were enrolled in this study. 
      Blood samples were collected from all patients. Additionally, all AA patients 
      underwent a bronchial allergen challenge (BAC) with Dermatophagoides 
      pteronyssinus, all of which were positive, and blood samples were collected again 
      24 h later. The concentrations of ten biologically active substances were 
      measured in the serum samples, using enzyme-linked immunosorbent assay (ELISA) 
      and the Luminex((R)) 100/200 System technology for bead-based multiplex and 
      singleplex immunoassays. Descriptive and analytical statistical methods were 
      used. A p-value of 0.05 or lower was considered statistically significant. The 
      soluble interleukin 5 receptor subunit alpha (sIL-5Ralpha) and thioredoxin 1 (TRX1) 
      concentrations were significantly increased, whereas those of tyrosine-protein 
      kinase Met (MET), pentraxin 3 (PTX3), and I C-telopeptide of type I collagen 
      (ICTP) were decreased in the AA group compared with the HS group. A significant 
      positive correlation was noted for sIL-5Ralpha with fractional exhaled nitric oxide 
      (Fe(NO)), blood eosinophil (EOS) count, and total immunoglobulin E (IgE) levels, 
      and a negative correlation was noted with forced expiratory volume in 1 s 
      (FEV(1)). Moreover, PTX3 showed negative correlations with blood EOS count and 
      total IgE levels, whereas ICTP exhibited a negative correlation with the blood 
      EOS count. In conclusion, this study demonstrated that the serum concentrations 
      of MET, PTX3, TRX1, ICTP, and particularly sIL-5Ralpha could potentially serve as 
      biomarkers of the AA phenotype.
FAU - Palacionyte, Jolita
AU  - Palacionyte J
AD  - Department of Pulmonology, Lithuanian University of Health Sciences, LT-44307 
      Kaunas, Lithuania.
FAU - Januskevicius, Andrius
AU  - Januskevicius A
AD  - Laboratory of Pulmonology, Department of Pulmonology, Lithuanian University of 
      Health Sciences, LT-44307 Kaunas, Lithuania.
FAU - Vasyle, Egle
AU  - Vasyle E
AD  - Laboratory of Pulmonology, Department of Pulmonology, Lithuanian University of 
      Health Sciences, LT-44307 Kaunas, Lithuania.
FAU - Rimkunas, Airidas
AU  - Rimkunas A
AUID- ORCID: 0000-0001-5418-3269
AD  - Laboratory of Pulmonology, Department of Pulmonology, Lithuanian University of 
      Health Sciences, LT-44307 Kaunas, Lithuania.
FAU - Bajoriuniene, Ieva
AU  - Bajoriuniene I
AD  - Department of Immunology and Allergology, Lithuanian University of Health 
      Sciences, LT-44307 Kaunas, Lithuania.
FAU - Vitkauskiene, Astra
AU  - Vitkauskiene A
AD  - Department of Laboratory Medicine, Lithuanian University of Health Sciences, 
      LT-44307 Kaunas, Lithuania.
FAU - Miliauskas, Skaidrius
AU  - Miliauskas S
AUID- ORCID: 0009-0002-6130-0712
AD  - Department of Pulmonology, Lithuanian University of Health Sciences, LT-44307 
      Kaunas, Lithuania.
FAU - Malakauskas, Kestutis
AU  - Malakauskas K
AUID- ORCID: 0000-0001-9141-2884
AD  - Department of Pulmonology, Lithuanian University of Health Sciences, LT-44307 
      Kaunas, Lithuania.
AD  - Laboratory of Pulmonology, Department of Pulmonology, Lithuanian University of 
      Health Sciences, LT-44307 Kaunas, Lithuania.
LA  - eng
GR  - (project number S-MIP-21-17)/Science Foundation of the Lithuanian University of 
      Health Sciences and Research Council of Lithuania/
PT  - Journal Article
DEP - 20240119
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC10813071
OTO - NOTNLM
OT  - allergic asthma
OT  - biomarker
OT  - eosinophil
OT  - fraction of exhaled nitric oxide
OT  - serum biologically active substance
OT  - total immunoglobulin E
COIS- The authors declare no conflicts of interest.
EDAT- 2024/01/26 12:43
MHDA- 2024/01/26 12:44
PMCR- 2024/01/19
CRDT- 2024/01/26 09:13
PHST- 2023/11/24 00:00 [received]
PHST- 2024/01/16 00:00 [revised]
PHST- 2024/01/17 00:00 [accepted]
PHST- 2024/01/26 12:44 [medline]
PHST- 2024/01/26 12:43 [pubmed]
PHST- 2024/01/26 09:13 [entrez]
PHST- 2024/01/19 00:00 [pmc-release]
AID - biomedicines12010232 [pii]
AID - biomedicines-12-00232 [pii]
AID - 10.3390/biomedicines12010232 [doi]
PST - epublish
SO  - Biomedicines. 2024 Jan 19;12(1):232. doi: 10.3390/biomedicines12010232.