PMID- 38278033 OWN - NLM STAT- MEDLINE DCOM- 20240227 LR - 20240227 IS - 1873-4367 (Electronic) IS - 0927-7765 (Linking) VI - 235 DP - 2024 Mar TI - Designing bioinspired multifunctional nanoplatforms to support wound healing and skin regeneration: Mg-hydroxyapatite meets melanins. PG - 113756 LID - S0927-7765(24)00014-6 [pii] LID - 10.1016/j.colsurfb.2024.113756 [doi] AB - Melanin is a multifunctional biological pigment that recently emerged as endowed with anti-inflammatory, antioxidant, and antimicrobial properties and with high potentialities in skin protection and regenerative medicine. Here, a biomimetic magnesium-doped nano-hydroxyapatite (MgHA) was synthesized and decorated with melanin molecules starting from two different monomeric precursors, i.e. 5,6-dihydroxyindole-2-carboxylic acid (DHICA) and dopamine (DA), demonstrating to be able to polymerize on the surface of MgHA nanostructures, thus leading to a melanin coating. This functionalization was realized by a simple and green preparation method requiring mild conditions in an aqueous medium and room temperature. Complementary spectroscopy and electron imaging analyses were carried out to define the effective formation of a stable coating, the percentage of the organic compounds, and the structural properties of resulting melanin-coated nanostructures, which showed good antioxidant activity. The in vitro interaction with a cell model, i.e. mouse fibroblasts, was investigated. The excellent biocompatibility of all bioinspired nanostructures was confirmed from a suitable cell proliferation. Finally, the enhanced biological performances of the nanostructures coated with melanin from DHICA were confirmed by scratch assays. Jointly our findings indicated that low crystalline MgHA and melanin pigments can be efficiently combined, and the resulting nanostructures are promising candidates as multifunctional platforms for a more efficient approach for skin regeneration and protection. CI - Copyright (c) 2024 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Furlani, Franco AU - Furlani F AD - National Research Council of Italy - Institute of Science, Technology and Sustainability for Ceramics - CNR - ISSMC (former ISTEC), Via Granarolo 64, I - 48018 Faenza (RA), Italy. Electronic address: franco.furlani@uniud.it. FAU - Pota, Giulio AU - Pota G AD - Department of Chemical, Materials and Production Engineering (DICMaPI), University of Naples Federico II, Piazzale V. Tecchio 80, 80125 Napoli, Italy and Bioelectronics Task Force at University of Naples Federico II, Naples, Italy. FAU - Rossi, Arianna AU - Rossi A AD - National Research Council of Italy - Institute of Science, Technology and Sustainability for Ceramics - CNR - ISSMC (former ISTEC), Via Granarolo 64, I - 48018 Faenza (RA), Italy; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, I-98166 Messina, Italy. FAU - Luciani, Giuseppina AU - Luciani G AD - Department of Chemical, Materials and Production Engineering (DICMaPI), University of Naples Federico II, Piazzale V. Tecchio 80, 80125 Napoli, Italy and Bioelectronics Task Force at University of Naples Federico II, Naples, Italy. FAU - Campodoni, Elisabetta AU - Campodoni E AD - National Research Council of Italy - Institute of Science, Technology and Sustainability for Ceramics - CNR - ISSMC (former ISTEC), Via Granarolo 64, I - 48018 Faenza (RA), Italy. FAU - Mocerino, Fabio AU - Mocerino F AD - Department of Chemical Sciences, University of Naples Federico II, via Cintia 21, I-80126 Napoli, Italy and Bioelectronics Task Force at University of Naples Federico II, Naples, Italy. FAU - D'Errico, Gerardino AU - D'Errico G AD - Department of Chemical Sciences, University of Naples Federico II, via Cintia 21, I-80126 Napoli, Italy and Bioelectronics Task Force at University of Naples Federico II, Naples, Italy; CSGI, Center for Colloid and Surface Science, via della Lastruccia 3, 50019 Florence, Italy. FAU - Pezzella, Alessandro AU - Pezzella A AD - Institute for Polymers Composites and Biomaterials (IPCB) CNR, Via Campi Flegrei 34, IT-80078 Pozzuoli (Na), Italy and Bioelectronics Task Force at University of Naples Federico II, Naples, Italy; National Interuniversity Consortium of Materials Science and Technology (INSTM), Piazza S. Marco, 4, Florence, Naples 50121, Italy; Department of Physics "Ettore Pancini", University of Naples Federico II, via Cintia 21, I-80126 Napoli, Italy, Via Campi Flegrei 34, IT-80078 Pozzuoli (Na), Italy and Bioelectronics Task Force at University of Naples Federico II, Naples, Italy. FAU - Panseri, Silvia AU - Panseri S AD - National Research Council of Italy - Institute of Science, Technology and Sustainability for Ceramics - CNR - ISSMC (former ISTEC), Via Granarolo 64, I - 48018 Faenza (RA), Italy. FAU - Vitiello, Giuseppe AU - Vitiello G AD - Department of Chemical, Materials and Production Engineering (DICMaPI), University of Naples Federico II, Piazzale V. Tecchio 80, 80125 Napoli, Italy and Bioelectronics Task Force at University of Naples Federico II, Naples, Italy; CSGI, Center for Colloid and Surface Science, via della Lastruccia 3, 50019 Florence, Italy. Electronic address: giuseppe.vitiello@unina.it. FAU - Sandri, Monica AU - Sandri M AD - National Research Council of Italy - Institute of Science, Technology and Sustainability for Ceramics - CNR - ISSMC (former ISTEC), Via Granarolo 64, I - 48018 Faenza (RA), Italy. LA - eng PT - Journal Article DEP - 20240115 PL - Netherlands TA - Colloids Surf B Biointerfaces JT - Colloids and surfaces. B, Biointerfaces JID - 9315133 RN - 0 (Melanins) RN - 0 (Indoles) RN - 0 (Antioxidants) RN - 0 (Hydroxyapatites) SB - IM MH - Animals MH - Mice MH - *Melanins/chemistry MH - *Indoles/pharmacology/chemistry MH - Antioxidants/pharmacology/chemistry MH - Wound Healing MH - Hydroxyapatites MH - Regeneration OTO - NOTNLM OT - Functional nano-bio-materials OT - Melanin OT - Nano-hydroxyapatite OT - Regenerative medicine OT - Skin protection OT - Wound healing COIS- Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Giuseppe Vitiello reports financial support was provided by University of Naples Federico II. EDAT- 2024/01/27 12:42 MHDA- 2024/02/27 06:44 CRDT- 2024/01/26 18:14 PHST- 2023/11/07 00:00 [received] PHST- 2024/01/04 00:00 [revised] PHST- 2024/01/12 00:00 [accepted] PHST- 2024/02/27 06:44 [medline] PHST- 2024/01/27 12:42 [pubmed] PHST- 2024/01/26 18:14 [entrez] AID - S0927-7765(24)00014-6 [pii] AID - 10.1016/j.colsurfb.2024.113756 [doi] PST - ppublish SO - Colloids Surf B Biointerfaces. 2024 Mar;235:113756. doi: 10.1016/j.colsurfb.2024.113756. Epub 2024 Jan 15.