PMID- 3827916
OWN - NLM
STAT- MEDLINE
DCOM- 19870422
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 143
IP  - 1
DP  - 1987 Feb 27
TI  - Photoregulation process of sorghum leaf phosphoenolpyruvate carboxylase: study 
      with monoclonal antibodies.
PG  - 170-7
AB  - Monoclonal antibodies were produced against the G isozyme subunit of PEP 
      carboxylase (PEPC) from Sorghum leaves by the hybridoma technique. More than 400 
      antibodies-producing hybridomas to PEPC were produced from the fusion of spleen 
      cells from immunized mice with NS1 myeloma cells. By using an ELISA, three 
      hybridomas (91-G, 83-G, 49-EG) were selected. Monoclonal antibodies were 
      subsequently characterized in a Western experiment; Mabs 83-G and 91-G were found 
      to be highly specific to the G isozyme whereas Mab 49-EG recognized both forms (E 
      and G isozymes) of the enzyme. Addition of Mabs to the enzyme preparation did not 
      modify its catalytic activity nor its activation by glycine. Use of these probes 
      provided direct and definite evidence of the specific enhancing effect of light 
      on the G form and on its corresponding mRNA.
FAU - Thomas, M
AU  - Thomas M
FAU - Cretin, C
AU  - Cretin C
FAU - Keryer, E
AU  - Keryer E
FAU - Vidal, J
AU  - Vidal J
FAU - Gadal, P
AU  - Gadal P
FAU - Bidart, J M
AU  - Bidart JM
FAU - Buhuon, C
AU  - Buhuon C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Isoenzymes)
RN  - 0 (Macromolecular Substances)
RN  - EC 4.1.1.- (Carboxy-Lyases)
RN  - EC 4.1.1.31 (Phosphoenolpyruvate Carboxylase)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Carboxy-Lyases/*radiation effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Isoenzymes/metabolism/radiation effects
MH  - Kinetics
MH  - Light
MH  - Macromolecular Substances
MH  - Phosphoenolpyruvate Carboxylase/genetics/metabolism/*radiation effects
MH  - Photochemistry
MH  - Plants/*enzymology/radiation effects
MH  - Protein Biosynthesis
EDAT- 1987/02/27 00:00
MHDA- 1987/02/27 00:01
CRDT- 1987/02/27 00:00
PHST- 1987/02/27 00:00 [pubmed]
PHST- 1987/02/27 00:01 [medline]
PHST- 1987/02/27 00:00 [entrez]
AID - 0006-291X(87)90646-2 [pii]
AID - 10.1016/0006-291x(87)90646-2 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1987 Feb 27;143(1):170-7. doi: 
      10.1016/0006-291x(87)90646-2.