PMID- 38282734
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240131
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 14
DP  - 2023
TI  - Identification of a novel B-cell epitope of the African swine fever virus p34 
      protein and development of an indirect ELISA for the detection of serum 
      antibodies.
PG  - 1308753
LID - 10.3389/fmicb.2023.1308753 [doi]
LID - 1308753
AB  - African swine fever (ASF) is a viral disease caused by the African swine fever 
      virus that can be highly transmitted and lethal in domestic pigs. In the absence 
      of a vaccine, effective diagnosis is critical for minimizing the virus's spread. 
      In recent years, with the decline of African swine fever virus (ASFV) virulence, 
      antibody detection has become an important means of detection. ASFV nucleocapsid 
      protein p34 is a mature hydrolytic product of pp220, which is highly conserved 
      and has a high content in the structural protein of the virus. Prokaryotic cells 
      were chosen to generate highly active and high-yield p34 protein, which was then 
      used as an antigen for producing mouse monoclonal antibodies. The B-cell epitope 
      (202)QKELDKLQT(210), which was highly conserved and found on the surface of the 
      p34 protein, was first identified by an anti-p34 monoclonal antibody utilizing 
      the peptide scanning technique and visualized in helix. This supported the 
      viability of p34 protein detection even further. In addition, we established an 
      indirect ELISA assay based on p34 to detect ASFV antibodies. The coincidence rate 
      of this method with commercially available kits was shown to be 97.83%. 
      Sensitivity analysis revealed that it could be detected in serum dilution as low 
      as 1:6400, and there was no cross-reaction with other prevalent porcine epidemic 
      diseases classical swine fever virus (CSFV), foot-and-mouth disease virus (FMDV), 
      porcine reproductive and respiratory syndrome virus (PRRSV), and porcine 
      circovirus 2 (PCV2). In summary, the established ELISA method and anti-P34 
      monoclonal antibody have demonstrated that the p34 protein has a promising 
      application prospect for the detection of African swine fever antibodies.
CI  - Copyright (c) 2024 Tian, Liang, Zhou, Sun, Liu, Chen, Zhu, Liu, Ding, Liu, Zhang, 
      Wu and Wang.
FAU - Tian, Yuanyuan
AU  - Tian Y
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Liang, Chao
AU  - Liang C
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Zhou, Jingming
AU  - Zhou J
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Sun, Fanglin
AU  - Sun F
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Liu, Yankai
AU  - Liu Y
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Chen, Yumei
AU  - Chen Y
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Zhu, Xifang
AU  - Zhu X
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Liu, Hongliang
AU  - Liu H
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Ding, Peiyang
AU  - Ding P
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Liu, Enping
AU  - Liu E
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Wu, Sixuan
AU  - Wu S
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
FAU - Wang, Aiping
AU  - Wang A
AD  - Longhu Laboratory, Zhengzhou, China.
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, China.
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20240112
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC10814126
OTO - NOTNLM
OT  - African swine fever virus
OT  - B-cell epitope
OT  - ELISA
OT  - antibody detection
OT  - monoclonal antibody
OT  - p34 protein
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2024/01/29 06:44
MHDA- 2024/01/29 06:45
PMCR- 2024/01/12
CRDT- 2024/01/29 04:06
PHST- 2023/10/07 00:00 [received]
PHST- 2023/12/18 00:00 [accepted]
PHST- 2024/01/29 06:45 [medline]
PHST- 2024/01/29 06:44 [pubmed]
PHST- 2024/01/29 04:06 [entrez]
PHST- 2024/01/12 00:00 [pmc-release]
AID - 10.3389/fmicb.2023.1308753 [doi]
PST - epublish
SO  - Front Microbiol. 2024 Jan 12;14:1308753. doi: 10.3389/fmicb.2023.1308753. 
      eCollection 2023.