PMID- 38284574 OWN - NLM STAT- MEDLINE DCOM- 20240130 LR - 20240515 IS - 1541-4337 (Electronic) IS - 1541-4337 (Linking) VI - 23 IP - 1 DP - 2024 Jan TI - Adding pulse flours to cereal-based snacks and bakery products: An overview of free asparagine quantification methods and mitigation strategies of acrylamide formation in foods. PG - e13260 LID - 10.1111/1541-4337.13260 [doi] AB - Thermal processing techniques can lead to the formation of heat-induced toxic substances. Acrylamide is one contaminant that has received much scientific attention in recent years, and it is formed essentially during the Maillard reaction when foods rich in carbohydrates, particularly reducing sugars (glucose, fructose), and certain free amino acids, especially asparagine (ASN), are processed at high temperatures (>120 degrees C). The highly variable free ASN concentration in raw materials makes it challenging for food businesses to keep acrylamide content below the European Commission benchmark levels, while avoiding flavor, color, and texture impacts on their products. Free ASN concentrations in crops are affected by environment, genotype, and soil fertilization, which can also influence protein content and amino acid composition. This review aims to provide an overview of free ASN and acrylamide quantification methods and mitigation strategies for acrylamide formation in foods, focusing on adding pulse flours to cereal-based snacks and bakery products. Overall, this review emphasizes the importance of these mitigation strategies in minimizing acrylamide formation in plant-based products and ensuring safer and healthier food options. CI - (c) 2023 The Authors. Comprehensive Reviews in Food Science and Food Safety published by Wiley Periodicals LLC on behalf of Institute of Food Technologists. FAU - Sa, Amanda G A AU - Sa AGA AUID- ORCID: 0000-0003-4654-7426 AD - Richardson Centre for Food Technology and Research, Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. FAU - House, James D AU - House JD AD - Richardson Centre for Food Technology and Research, Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. LA - eng GR - Ministry of Agriculture - Saskatchewan/ GR - Genome Canada/ PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review PL - United States TA - Compr Rev Food Sci Food Saf JT - Comprehensive reviews in food science and food safety JID - 101305205 RN - 7006-34-0 (Asparagine) RN - 20R035KLCI (Acrylamide) RN - 0 (Carbohydrates) RN - 0 (Amino Acids) SB - IM MH - *Asparagine/analysis/chemistry/metabolism MH - *Edible Grain/chemistry MH - Acrylamide/analysis/chemistry/toxicity MH - Snacks MH - Carbohydrates/analysis/chemistry MH - Amino Acids/analysis OTO - NOTNLM OT - Maillard reaction OT - chromatography OT - environment OT - free asparagine OT - genotype OT - legumes OT - soil fertilization EDAT- 2024/01/29 12:44 MHDA- 2024/01/30 12:44 CRDT- 2024/01/29 08:13 PHST- 2023/09/30 00:00 [revised] PHST- 2023/07/21 00:00 [received] PHST- 2023/10/13 00:00 [accepted] PHST- 2024/01/30 12:44 [medline] PHST- 2024/01/29 12:44 [pubmed] PHST- 2024/01/29 08:13 [entrez] AID - 10.1111/1541-4337.13260 [doi] PST - ppublish SO - Compr Rev Food Sci Food Saf. 2024 Jan;23(1):e13260. doi: 10.1111/1541-4337.13260.