PMID- 38285408
OWN - NLM
STAT- MEDLINE
DCOM- 20240417
LR  - 20240417
IS  - 1573-5168 (Electronic)
IS  - 0920-1742 (Linking)
VI  - 50
IP  - 2
DP  - 2024 Apr
TI  - Eicosapentaenoic acid (EPA) improves grass carp (Ctenopharyngodon idellus) muscle 
      development and nutritive value by activating the mTOR signaling pathway.
PG  - 687-703
LID - 10.1007/s10695-024-01299-5 [doi]
AB  - Skeletal muscle is the mainly edible part of fish. Eicosapentaenoic acid (EPA) is 
      a crucial nutrient for fish. This study investigated the effect of EPA on the 
      muscle development of grass carp along with the potential molecular mechanisms in 
      vivo and in vitro. Muscle cells treated with 50 muM EPA in vitro showed the 
      elevated proliferation, and the expression of mammalian target of rapamycin 
      (mTOR) signaling pathway-related genes was upregulated (P < 0.05). In vivo 
      experiments, 270 grass carp (27.92 g) were fed with one of the three experimental 
      diets for 56 days: control diet (CN), 0.3% EPA-supplement diet (EPA), and the 
      diet supplemented with 0.3% EPA and 30 mg/kg rapamycin (EPA + Rap). Fish weight 
      gain rate (WGR) was improved in EPA group (P < 0.05). There was no difference in 
      the viscerosomatic index (VSI) and body height (BH) among all groups (P > 0.05), 
      whereas the carcass ratio (CR) and body length in the EPA group were obviously 
      higher than those of other groups (P < 0.05), indicating that the increase of WGR 
      was due to muscle growth. In addition, both muscle fiber density and muscle crude 
      protein also increased in EPA group (P < 0.05). The principal component analysis 
      showed that total weight of muscle amino acid in EPA group ranked first. Dietary 
      EPA also increased protein levels of the total mTOR, S6k1, Myhc, Myog, and Myod 
      in muscle (P < 0.05). In conclusion, EPA promoted the muscle development and 
      nutritive value via activating the mTOR signaling pathway.
CI  - (c) 2024. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Li, Handong
AU  - Li H
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Xianyang, 712100, Shanxi, China.
FAU - Ji, Shanghong
AU  - Ji S
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Xianyang, 712100, Shanxi, China.
FAU - Yuan, Xiangtong
AU  - Yuan X
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Xianyang, 712100, Shanxi, China.
FAU - Li, Yunhe
AU  - Li Y
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Xianyang, 712100, Shanxi, China.
FAU - Kaneko, Gen
AU  - Kaneko G
AD  - College of Natural and Applied Science, University of Houston-Victoria, Victoria, 
      Texas, USA.
FAU - Sun, Jian
AU  - Sun J
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Xianyang, 712100, Shanxi, China.
FAU - Ji, Hong
AU  - Ji H
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Xianyang, 712100, Shanxi, China. jihong@nwsuaf.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20240129
PL  - Netherlands
TA  - Fish Physiol Biochem
JT  - Fish physiology and biochemistry
JID - 100955049
RN  - AAN7QOV9EA (Eicosapentaenoic Acid)
RN  - 0 (Dietary Proteins)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - 0 (Fish Proteins)
SB  - IM
MH  - Animals
MH  - *Eicosapentaenoic Acid/pharmacology/analysis
MH  - *Carps/metabolism
MH  - Signal Transduction
MH  - Diet
MH  - Muscle, Skeletal/metabolism
MH  - Dietary Proteins
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
MH  - Muscle Development
MH  - Nutritive Value
MH  - Animal Feed/analysis
MH  - Fish Proteins/genetics
MH  - Mammals/metabolism
OTO - NOTNLM
OT  - Eicosapentaenoic acid
OT  - Muscle growth
OT  - Myoblasts
OT  - Principal component analysis
OT  - Rapamycin
EDAT- 2024/01/29 12:44
MHDA- 2024/04/17 06:42
CRDT- 2024/01/29 11:23
PHST- 2023/10/04 00:00 [received]
PHST- 2024/01/10 00:00 [accepted]
PHST- 2024/04/17 06:42 [medline]
PHST- 2024/01/29 12:44 [pubmed]
PHST- 2024/01/29 11:23 [entrez]
AID - 10.1007/s10695-024-01299-5 [pii]
AID - 10.1007/s10695-024-01299-5 [doi]
PST - ppublish
SO  - Fish Physiol Biochem. 2024 Apr;50(2):687-703. doi: 10.1007/s10695-024-01299-5. 
      Epub 2024 Jan 29.