PMID- 38286072
OWN - NLM
STAT- MEDLINE
DCOM- 20240208
LR  - 20240208
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 128
DP  - 2024 Feb 15
TI  - Crocetin inhibits mast cell-dependent immediate-type allergic reactions through 
      Ca(2+)/PLC/IP3 and TNF pathway.
PG  - 111583
LID - S1567-5769(24)00101-2 [pii]
LID - 10.1016/j.intimp.2024.111583 [doi]
AB  - Crocetin is a kind of glycocone naturally occurring in Crocus sativus L.. It is 
      an active metabolite produced by biohydrolysis of Crocus sativus L.. Crocetin has 
      anti-cardiovascular diseases and antioxidant effects, but its anti-allergic 
      effect has not been reported. In this study, the inhibitory effect of crocetin on 
      immunoglobulin E (IgE) - mediated allergic reaction and the mechanism of action 
      were investigated. The passive cutaneous anaphylaxis (PCA) was used to elucidate 
      the anti-allergic effects of crocetin in vivo. Degranulation assay, calcium 
      imaging, and cytokine release assay were to evaluate the anti-allergic effect of 
      crocetin in vitro. We found that crocetin IgE-mediated RBL-2H3 cell degranulation 
      and allergy both in vitro and in vivo. The TNF pathway was inhibited by crocetin 
      in our RNA-seq sequences, Furthermore, crocetin inhibits IgE-mediated calcium 
      influx, and PLC / IP3 phosphorylation in RBL-2H3 cells. Our findings suggested 
      that crocetin revealed prominent anti-allergy activity through TNF and 
      Ca(2+)/PLC/IP3 pathway.
CI  - Copyright (c) 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
FAU - Fan, Ting
AU  - Fan T
AD  - Department of Clinical Pharmacy, Honghui Hospital, Xi'an Jiaotong University, 
      Xi'an, Shaanxi Province, China.
FAU - Jiang, Kai
AU  - Jiang K
AD  - Department of Clinical Pharmacy, Honghui Hospital, Xi'an Jiaotong University, 
      Xi'an, Shaanxi Province, China.
FAU - Wang, Zixiao
AU  - Wang Z
AD  - Department of Clinical Pharmacy, Honghui Hospital, Xi'an Jiaotong University, 
      Xi'an, Shaanxi Province, China.
FAU - Chang, Yu
AU  - Chang Y
AD  - Department of Clinical Pharmacy, Honghui Hospital, Xi'an Jiaotong University, 
      Xi'an, Shaanxi Province, China.
FAU - Tian, Hua
AU  - Tian H
AD  - Department of Respiratory and geriatrics, Honghui Hospital, Xi'an Jiaotong 
      University, Xi'an, Shaanxi Province, China. Electronic address: 
      tianhua80@sina.com.
FAU - Huang, Jing
AU  - Huang J
AD  - Department of Clinical Pharmacy, Honghui Hospital, Xi'an Jiaotong University, 
      Xi'an, Shaanxi Province, China. Electronic address: huangjing87206@126.com.
LA  - eng
PT  - Journal Article
DEP - 20240128
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - SY7Q814VUP (Calcium)
RN  - 0 (trans-sodium crocetinate)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (Anti-Allergic Agents)
RN  - 36-88-4 (Carotenoids)
RN  - 11103-57-4 (Vitamin A)
SB  - IM
MH  - Humans
MH  - Mast Cells
MH  - Calcium/metabolism
MH  - *Hypersensitivity/drug therapy
MH  - Immunoglobulin E/metabolism
MH  - *Hypersensitivity, Immediate
MH  - *Anti-Allergic Agents/therapeutic use
MH  - Cell Degranulation
MH  - *Carotenoids
MH  - Vitamin A/*analogs & derivatives
OTO - NOTNLM
OT  - Allergy
OT  - Crocetin
OT  - Mast cell
OT  - TNF pathway
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2024/01/29 19:57
MHDA- 2024/02/08 06:43
CRDT- 2024/01/29 18:02
PHST- 2023/11/29 00:00 [received]
PHST- 2024/01/21 00:00 [revised]
PHST- 2024/01/22 00:00 [accepted]
PHST- 2024/02/08 06:43 [medline]
PHST- 2024/01/29 19:57 [pubmed]
PHST- 2024/01/29 18:02 [entrez]
AID - S1567-5769(24)00101-2 [pii]
AID - 10.1016/j.intimp.2024.111583 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2024 Feb 15;128:111583. doi: 10.1016/j.intimp.2024.111583. 
      Epub 2024 Jan 28.