PMID- 38286878
OWN - NLM
STAT- MEDLINE
DCOM- 20240427
LR  - 20240430
IS  - 1432-5233 (Electronic)
IS  - 0940-5429 (Print)
IS  - 0940-5429 (Linking)
VI  - 61
IP  - 5
DP  - 2024 May
TI  - Association of serum CTRP4 levels with vascular endothelial function in patients 
      with type 2 diabetes mellitus: CTRP4 ameliorating inflammation, proliferation and 
      migration in human umbilical vein endothelial cells.
PG  - 565-575
LID - 10.1007/s00592-023-02228-3 [doi]
AB  - OBJECTIVE: We investigated the correlation between serum C1q/TNF-related protein 
      4 (CTRP4) level and flow-mediated dilation (FMD) in patients with type 2 diabetes 
      mellitus (T2DM), and evaluated the biological effects of CTRP4 on human umbilical 
      vein endothelial cells (HUVECs). METHODS: A group of 165 patients diagnosed with 
      T2DM were included in this study. Endothelial function was measured with the 
      examination of brachial artery FMD. ELISA kit was used to measure the levels of 
      CTRP4 in serum. HUVECs were stimulated with recombinant CTRP4 protein to assess 
      its biological functions. RESULTS: The levels of CTRP4 showed a significant 
      variation among three groups based on FMD tertiles (p = 0.001). What's more, FMD 
      had a significant difference among three CTRP4 tertile groups (p < 0.05) and was 
      negatively related to serum CTRP4 levels (r = -0.270, p < 0.001). In T2DM 
      patients, logistic regression analysis demonstrated that CTRP4 was the primary 
      influence factor of low FMD (p < 0.01). In receiver operating characteristic 
      curve analysis, the area under the curve of CTRP4 for predicting low FMD was 0.66 
      (95%CI 0.58-0.75). When stimulated HUVECs with recombinant CTRP4 protein, we 
      found that CTRP4 could concentration-dependently ameliorate proliferation and 
      migration of HUVECs in wounding healing and transwell assay. This protein could 
      also decrease the expression of IL-6 and TNF-alpha and promote the release of NO in 
      HUVEC supernatants, with suppression of NF-kappaB and STAT3 phosphorylation. 
      CONCLUSIONS: Serum CTRP4 concentrations were negatively associated with FMD. 
      CTRP4 alleviated proliferation, migration and inflammation in HUVECs through the 
      suppression of NF-kappaB and STAT3 signaling pathways.
CI  - (c) 2024. The Author(s).
FAU - Gao, Jie
AU  - Gao J
AUID- ORCID: 0000-0003-3330-2219
AD  - Department of Endocrinology, Putuo Hospital, Shanghai University of Traditional 
      Chinese Medicine, 164 LanXi Road, Shanghai, 200062, China.
FAU - Rouzi, Mai Re YanMu
AU  - Rouzi MRY
AD  - School of Medical and Life Sciences, Chengdu University of Traditional Chinese 
      Medicine, Chengdu, China.
FAU - Zhang, Huihui
AU  - Zhang H
AD  - School of Medical and Life Sciences, Chengdu University of Traditional Chinese 
      Medicine, Chengdu, China.
FAU - Cai, Xinghua
AU  - Cai X
AD  - Shanghai Putuo Center School of Clinical Medicine, Anhui Medical University, 
      Hefei, Anhui, China.
FAU - Xu, Bilin
AU  - Xu B
AD  - Department of Endocrinology, Putuo Hospital, Shanghai University of Traditional 
      Chinese Medicine, 164 LanXi Road, Shanghai, 200062, China.
FAU - Lu, Jun
AU  - Lu J
AD  - Department of Endocrinology, Putuo Hospital, Shanghai University of Traditional 
      Chinese Medicine, 164 LanXi Road, Shanghai, 200062, China.
FAU - Lei, Tao
AU  - Lei T
AD  - Department of Endocrinology, Putuo Hospital, Shanghai University of Traditional 
      Chinese Medicine, 164 LanXi Road, Shanghai, 200062, China. taolei_12@sina.com.
LA  - eng
GR  - 2020tszk01/Clinical Characteristic of Health System in Putuo District, Shanghai/
GR  - ZK2019B16/Shanghai Medical Key Specialties/
GR  - ptkwws202003/Science, Technology Innovation Project of Putuo District Health 
      System/
GR  - ptkwws201911/Science, Technology Innovation Project of Putuo District Health 
      System/
GR  - 20204Y0154/Research Project of Shanghai Municipal Health Care Commission/
GR  - 22-LY-03/Scientific Research of Shanghai Sixth Hospital Consortium/
PT  - Journal Article
DEP - 20240129
PL  - Germany
TA  - Acta Diabetol
JT  - Acta diabetologica
JID - 9200299
RN  - 0 (Adipokines)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Humans
MH  - *Human Umbilical Vein Endothelial Cells
MH  - *Diabetes Mellitus, Type 2/blood
MH  - Male
MH  - Female
MH  - Middle Aged
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - *Inflammation
MH  - Endothelium, Vascular/metabolism
MH  - Aged
MH  - Adipokines/blood
MH  - Adult
MH  - NF-kappa B/metabolism
PMC - PMC11055794
OTO - NOTNLM
OT  - CTRP4
OT  - Endothelial function
OT  - Flow-mediated dilation
OT  - Type 2 diabetes mellitus
COIS- The authors declare that there are no conflict of interests.
EDAT- 2024/01/30 00:42
MHDA- 2024/04/28 07:24
PMCR- 2024/01/29
CRDT- 2024/01/29 23:18
PHST- 2023/10/31 00:00 [received]
PHST- 2023/12/18 00:00 [accepted]
PHST- 2024/04/28 07:24 [medline]
PHST- 2024/01/30 00:42 [pubmed]
PHST- 2024/01/29 23:18 [entrez]
PHST- 2024/01/29 00:00 [pmc-release]
AID - 10.1007/s00592-023-02228-3 [pii]
AID - 2228 [pii]
AID - 10.1007/s00592-023-02228-3 [doi]
PST - ppublish
SO  - Acta Diabetol. 2024 May;61(5):565-575. doi: 10.1007/s00592-023-02228-3. Epub 2024 
      Jan 29.