PMID- 38287796 OWN - NLM STAT- MEDLINE DCOM- 20240131 LR - 20240219 IS - 2768-6698 (Electronic) IS - 2768-6698 (Linking) VI - 29 IP - 1 DP - 2024 Jan 12 TI - SIRT6 Reduces Rheumatoid Arthritis Injury by Inhibiting MyD88-ERK Signaling Pathway. PG - 5 LID - 10.31083/j.fbl2901005 [doi] AB - BACKGROUND: Rheumatoid arthritis (RA) is an autoimmune disease characterized by destruction of synovial joints, abnormal immune responses and chronic inflammatory manifestations, which seriously affects patients' well-being. We explored this study to ascertain the effect and mechanism of silent information regulator 6 (SIRT6) on RA. METHODS: Genes of RA patients and normal volunteers were analyzed using Gene Expression Omnibus (GEO), Kyoto-Encyclopedia of Genes and Genomes (KEGG) and Disconet databases. Serum samples of RA patients and normal subjects were collected before detection of myeloid differentiation factor-88 (MyD88)-extracellular signal-regulated kinase (ERK) pathway proteins expression with Western blot. In vitro RA fibroblast-like synoviocytes (FLS) cell model (RA-FLS) was established by treating RSC-364 with recombinant rat IL-1beta (10 ng/mL) after which SIRT6 and MyD88 adenoviruses treatment was carried out. The enzyme linked immunoassay (ELISA), real time polymerase chain reaction (RT-PCR) and Western blot were respectively used to measure inflammatory factors, related messenger ribonucleic acid (mRNA) and protein expressions. Also, we constructed RA rat model with bovine type II collagen (BIIC) and complete Freund's adjuvant, before treatment with SIRT6 and MyD88 adenoviruses. RESULTS: Low expression of SIRT6 gene were detected in RA patients. Also, levels of MyD88, ERK and phosphorylated extracellular signal-regulated protein kinase (p-ERK) protein expressions in RA patients were increased, whilst that of SIRT6 protein decreased. Compared to FLS cells in Control group, inflammatory factors levels of rats in Model batch increased significantly. SIRT6 adenovirus treatment potentially and significantly inhibited inflammation including suppression of increased inflammatory factors induced by MyD88. In comparison with FLS cells in Control group, Model batch cells' MyD88, interleukin (IL)-1beta, IL-21, IL-22, IL-6, IL-17, tumor necrosis factor-alpha (TNF-alpha) and monocyte chemo-attractant protein-1 (MCP-1) mRNA expressions increased but SIRT6 gene treatment could reduce mRNA expression of the aforesaid factors, even after MyD88 adenovirus treatment. Besides, overpressed SIRT6 negatively regulated levels of MyD88, ERK and p-ERK proteins expressions. SIRT6 demonstrated anti-RA effect by regulating MyD88-ERK pathway and inhibiting inflammatory response in RA rats. CONCLUSIONS: SIRT6 could potentially inhibit the inflammatory response of RA via a regulatory mechanism mainly relating to MyD88-ERK signal pathway. Thus, SIRT6 and its agonists may serve as new targets for developing drugs that can potentially treat RA. CI - (c) 2024 The Author(s). Published by IMR Press. FAU - Yu, Xiaolong AU - Yu X AD - Department of Ultrasound, Xuzhou Medical University, Wujin Clinical College, 213000 Changzhou, Jiangsu, China. AD - Science and Education Section, Jiangsu University Affiliated Wujin Hospital, 213006 Changzhou, Jiangsu, China. AD - Jiangsu Key Laboratory of Immunity and Metabolism, Xuzhou Medical University, 210000 Xuzhou, Jiangsu, China. FAU - Jin, Zihan AU - Jin Z AD - Department of Clinical laboratory, Nanjing Medical University Affiliated Changzhou Second People's Hospital, 211166 Changzhou, Jiangsu, China. FAU - Raza, Faisal AU - Raza F AD - School of Pharmacy, Shanghai Jiao Tong University, 200240 Shanghai, China. FAU - Zhang, Ping AU - Zhang P AD - Department of Clinical laboratory, Nanjing Medical University Affiliated Changzhou Second People's Hospital, 211166 Changzhou, Jiangsu, China. FAU - Wu, Jiabiao AU - Wu J AD - Science and Education Section, Jiangsu University Affiliated Wujin Hospital, 213006 Changzhou, Jiangsu, China. FAU - Ren, Min AU - Ren M AD - Science and Education Section, Jiangsu University Affiliated Wujin Hospital, 213006 Changzhou, Jiangsu, China. FAU - Wang, Jiapeng AU - Wang J AD - Department of Pharmaceutics, School of Pharmacy, Jiangsu University, 212013 Zhenjiang, Jiangsu, China. FAU - Xi, Jing AU - Xi J AD - Department of Ultrasound, Xuzhou Medical University, Wujin Clinical College, 213000 Changzhou, Jiangsu, China. AD - Science and Education Section, Jiangsu University Affiliated Wujin Hospital, 213006 Changzhou, Jiangsu, China. LA - eng GR - CZQM2020120/Young Talent Development Plan of Changzhou Health Commission/ GR - XZSYSKF2020018/Jiangsu Key Laboratory of Immunology and Metabolism/ GR - 2022CZBJ109/Top Talent of Changzhou "The 14th Five-Year Plan" High-Level Health Talents Training Project/ GR - NMUB20220194/Nanjing Medical University Science and Technology Development Fund/ GR - CJ20220164/Changzhou Sci & Tech Program/ GR - JDYY2023074/Clinical Technology Development Foundation of Jiangsu University/ PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Singapore TA - Front Biosci (Landmark Ed) JT - Frontiers in bioscience (Landmark edition) JID - 101612996 RN - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases) RN - 0 (Myeloid Differentiation Factor 88) RN - 0 (RNA, Messenger) RN - EC 3.5.1.- (Sirtuins) RN - EC 3.5.1.- (SIRT6 protein, human) SB - IM MH - Humans MH - Animals MH - Cattle MH - Rats MH - Extracellular Signal-Regulated MAP Kinases/metabolism MH - Myeloid Differentiation Factor 88/genetics/metabolism/pharmacology MH - *Arthritis, Rheumatoid/genetics MH - Signal Transduction MH - Inflammation/metabolism MH - RNA, Messenger/metabolism MH - *Sirtuins/genetics/metabolism/pharmacology MH - Fibroblasts/metabolism MH - Cells, Cultured OTO - NOTNLM OT - MyD88-ERK signaling pathway OT - SIRT6 OT - rheumatoid arthritis OT - ultrasonic OT - western blot COIS- The authors declare no conflict of interest. EDAT- 2024/01/30 06:42 MHDA- 2024/01/31 06:43 CRDT- 2024/01/30 02:53 PHST- 2023/05/19 00:00 [received] PHST- 2023/08/17 00:00 [revised] PHST- 2023/08/25 00:00 [accepted] PHST- 2024/01/31 06:43 [medline] PHST- 2024/01/30 06:42 [pubmed] PHST- 2024/01/30 02:53 [entrez] AID - S2768-6701(23)01021-3 [pii] AID - 10.31083/j.fbl2901005 [doi] PST - ppublish SO - Front Biosci (Landmark Ed). 2024 Jan 12;29(1):5. doi: 10.31083/j.fbl2901005.