PMID- 3828983
OWN - NLM
STAT- MEDLINE
DCOM- 19870520
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 34
IP  - 3
DP  - 1987 Mar
TI  - Active H-ras and N-ras in rat fibrosarcomas induced by 1,6-dinitropyrene.
PG  - 317-24
AB  - The activated oncogenes in rat fibrosarcomas induced by subcutaneous injection of 
      1,6-dinitropyrene (1,6-DNP), an environmental mutagen-carcinogen, were examined 
      by NIH3T3 cell transfection assay and Southern blot analysis. DNAs from two of 
      eleven fibrosarcomas, 1,6-DNP6 and 1,6-DNP9T, induced transformants which 
      contained rat specific repetitive sequences. Three primary transformants, 
      obtained with DNA from 1,6-DNP6, and two secondary transformants of one of these 
      primary transformants tested, contained rat H-ras. DNA from 1,6-DNP9T; a fourth 
      transplant of 1,6-DNP9, also induced transformants. All the primary transformants 
      with DNA from 1,6-DNP9T, and three secondary transformants of one of them, 
      contained rat N-ras. The DNA from the original tumor, 1,6-DNP9, however, did not 
      give any transformants by several transfection assays. Activation of N-ras 
      oncogene during serial transplantations is discussed.
FAU - Ishizaka, Y
AU  - Ishizaka Y
FAU - Ochiai, M
AU  - Ochiai M
FAU - Ohgaki, H
AU  - Ohgaki H
FAU - Ishikawa, F
AU  - Ishikawa F
FAU - Sato, S
AU  - Sato S
FAU - Miura, Y
AU  - Miura Y
FAU - Nagao, M
AU  - Nagao M
FAU - Sugimura, T
AU  - Sugimura T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Mutagens)
RN  - 0 (Pyrenes)
RN  - 66Q2ZUF83N (1,6-dinitropyrene)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA/analysis
MH  - Fibrosarcoma/chemically induced/*genetics
MH  - Male
MH  - *Mutagens
MH  - *Oncogenes
MH  - Pyrenes/*toxicity
MH  - Rats
MH  - Rats, Inbred F344
MH  - Transfection
EDAT- 1987/03/01 00:00
MHDA- 1987/03/01 00:01
CRDT- 1987/03/01 00:00
PHST- 1987/03/01 00:00 [pubmed]
PHST- 1987/03/01 00:01 [medline]
PHST- 1987/03/01 00:00 [entrez]
AID - 0304-3835(87)90182-0 [pii]
AID - 10.1016/0304-3835(87)90182-0 [doi]
PST - ppublish
SO  - Cancer Lett. 1987 Mar;34(3):317-24. doi: 10.1016/0304-3835(87)90182-0.