PMID- 38291955
OWN - NLM
STAT- MEDLINE
DCOM- 20240201
LR  - 20240206
IS  - 1547-6901 (Electronic)
IS  - 1547-691X (Linking)
VI  - 21
IP  - 1
DP  - 2024 Dec
TI  - Identification and semi-quantification of protein allergens in complex mixtures 
      using proteomic and AllerCatPro 2.0 bioinformatic analyses: a proof-of-concept 
      investigation.
PG  - 2305452
LID - 10.1080/1547691X.2024.2305452 [doi]
AB  - The demand for botanicals and natural substances in consumer products has 
      increased in recent years. These substances usually contain proteins and these, 
      in turn, can pose a risk for immunoglobulin E (IgE)-mediated sensitization and 
      allergy. However, no method has yet been accepted or validated for assessment of 
      potential allergenic hazards in such materials. In the studies here, a dual 
      proteomic-bioinformatic approach is proposed to evaluate holistically allergenic 
      hazards in complex mixtures of plants, insects, or animal proteins. Twelve 
      commercial preparations of source materials (plant products, dust mite extract, 
      and preparations of animal dander) known to contain allergenic proteins were 
      analyzed by label-free proteomic analyses to identify and semi-quantify proteins. 
      These were then evaluated by bioinformatics using AllerCatPro 2.0 
      (https://allercatpro.bii.a-star.edu.sg/) to predict no, weak, or strong evidence 
      for allergenicity and similarity to source-specific allergens. In total, 4,586 
      protein sequences were identified in the 12 source materials combined. Of these, 
      1,665 sequences were predicted with weak or strong evidence for allergenic 
      potential. This first-tier approach provided top-level information about the 
      occurrence and abundance of proteins and potential allergens. With regards to 
      source-specific allergens, 129 allergens were identified. The sum of the relative 
      abundance of these allergens ranged from 0.8% (lamb's quarters) to 63% (olive 
      pollen). It is proposed here that this dual proteomic-bioinformatic approach has 
      the potential to provide detailed information on the presence and relative 
      abundance of allergens, and can play an important role in identifying potential 
      allergenic hazards in complex protein mixtures for the purposes of safety 
      assessments.
FAU - Krutz, Nora L
AU  - Krutz NL
AD  - NV Procter & Gamble Services Company SA, Global Product Stewardship, 
      Strombeek-Bever, Belgium.
FAU - Kimber, Ian
AU  - Kimber I
AD  - Faculty of Biology, Medicine and Health, University of Manchester, Manchester, 
      UK.
FAU - Winget, Jason
AU  - Winget J
AD  - The Procter & Gamble Company, Mason, OH, USA.
FAU - Nguyen, Minh N
AU  - Nguyen MN
AD  - Agency for Science, Technology and Research (A*STAR), Bioinformatics Institute, 
      Singapore, Singapore.
FAU - Limviphuvadh, Vachiranee
AU  - Limviphuvadh V
AD  - Agency for Science, Technology and Research (A*STAR), Bioinformatics Institute, 
      Singapore, Singapore.
FAU - Maurer-Stroh, Sebastian
AU  - Maurer-Stroh S
AD  - Agency for Science, Technology and Research (A*STAR), Bioinformatics Institute, 
      Singapore, Singapore.
AD  - Yong Loo Lin School of Medicine and Department of Biological Sciences, National 
      University of Singapore (NUS), Singapore, Singapore.
FAU - Mahony, Catherine
AU  - Mahony C
AD  - Procter & Gamble, Global Product Stewardship, Egham, UK.
FAU - Gerberick, G Frank
AU  - Gerberick GF
AD  - GF3 Consultancy LLC, West Chester, OH, USA.
LA  - eng
PT  - Journal Article
DEP - 20240131
PL  - England
TA  - J Immunotoxicol
JT  - Journal of immunotoxicology
JID - 101201960
RN  - 0 (Allergens)
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - *Allergens
MH  - Proteomics
MH  - Proteins
MH  - Amino Acid Sequence
MH  - *Hypersensitivity
OTO - NOTNLM
OT  - IgE
OT  - Protein allergenicity
OT  - botanicals
OT  - natural substances
OT  - risk assessment
EDAT- 2024/01/31 06:43
MHDA- 2024/02/01 06:42
CRDT- 2024/01/31 03:42
PHST- 2024/02/01 06:42 [medline]
PHST- 2024/01/31 06:43 [pubmed]
PHST- 2024/01/31 03:42 [entrez]
AID - 10.1080/1547691X.2024.2305452 [doi]
PST - ppublish
SO  - J Immunotoxicol. 2024 Dec;21(1):2305452. doi: 10.1080/1547691X.2024.2305452. Epub 
      2024 Jan 31.