PMID- 38293055
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240131
DP  - 2024 Jan 16
TI  - Elucidation of neuronal activity in mouse models of TMJ injury by in vivo GCaMP 
      Ca (2+) imaging of intact trigeminal ganglion neurons.
LID - 2024.01.16.575919 [pii]
LID - 10.1101/2024.01.16.575919 [doi]
AB  - Patients with temporomandibular disorders (TMD) typically experience facial pain 
      and discomfort or tenderness in the temporomandibular joint (TMJ), causing 
      disability in daily life. Unfortunately, existing treatments for TMD are not 
      always effective, creating a need for more advanced, mechanism-based therapies. 
      In this study, we used in vivo GCaMP3 Ca (2+) imaging of intact trigeminal 
      ganglia (TG) to characterize functional activity of the TG neurons in vivo , 
      specifically in TMJ animal models. This system allows us to observe neuronal 
      activity in intact anatomical, physiological, and clinical conditions and to 
      assess neuronal function and response to various stimuli. We observed a 
      significant increase in spontaneously and transiently activated neurons 
      responding to mechanical, thermal, and chemical stimuli in the TG of forced mouth 
      open (FMO) mice. An inhibitor of the CGRP receptor significantly attenuated 
      FMO-induced facial hypersensitivity. In addition, we confirmed the attenuating 
      effect of CGRP antagonist on FMO-induced sensitization by in vivo GCaMP3 Ca (2+) 
      imaging of intact TG. Our results contribute to unraveling the role and activity 
      of TG neurons in the TMJ pain animal models of TMD, bringing us closer 
      understanding the pathophysiological processes underlying TMD. Our study also 
      illustrates the utility of in vivo GCaMP3 Ca (2+) imaging of intact TG for 
      studies aimed at developing more targeted and effective treatments for TMD.
FAU - Son, Hyeonwi
AU  - Son H
FAU - Shannonhouse, John
AU  - Shannonhouse J
FAU - Zhang, Yan
AU  - Zhang Y
FAU - Gomez, Ruben
AU  - Gomez R
FAU - Chung, Man-Kyo
AU  - Chung MK
FAU - Kim, Yu Shin
AU  - Kim YS
LA  - eng
PT  - Preprint
DEP - 20240116
PL  - United States
TA  - bioRxiv
JT  - bioRxiv : the preprint server for biology
JID - 101680187
PMC - PMC10827170
EDAT- 2024/01/31 06:42
MHDA- 2024/01/31 06:43
PMCR- 2024/01/30
CRDT- 2024/01/31 04:19
PHST- 2024/01/31 06:43 [medline]
PHST- 2024/01/31 06:42 [pubmed]
PHST- 2024/01/31 04:19 [entrez]
PHST- 2024/01/30 00:00 [pmc-release]
AID - 2024.01.16.575919 [pii]
AID - 10.1101/2024.01.16.575919 [doi]
PST - epublish
SO  - bioRxiv [Preprint]. 2024 Jan 16:2024.01.16.575919. doi: 
      10.1101/2024.01.16.575919.